Radioimmunoassay of Atrial Peptide Blood and Tissue Levels
The observation (DeBold et al. 1981) that mammalian atria contain a peptide that is both natriuretic and diuretic has led to the discovery of a novel endocrine hormone (atriopeptin, AP) which regulates salt and water metabolism from the kidney (Needleman et al. 1985). Atrial extracts were found to be both heat and acid stable (Debold et al. 1981), and to possess spasmolytic activity on vascular smooth muscle (Currie et al. 1983). These bioassays facilitated the purification and sequence analysis of three related peptides, termed AP I, II, and III (Currie et al. 1984 a). Several peptides of different lengths, but sharing a common core sequence to the APs, have been identified by different laboratories. These include cardionatrin (Flynn et a. 1983), atrial natriuretic factor (ANF) (Thibault et al. 1983), atrial natriuretic polypeptide (ANP) (Kangawa and Matsuo 1984), and auriculin (Atlas et al. 1984). cDNA probes were constructed from the peptide sequence and used to determine the sequence for the AP gene (Yamanaka et al. 1984; Maki et al. 1984; Oikawa et al. 1984; Seidman et al. 1984). The AP gene codes for a 152 amino acid preprohormone which contains a hydrophobic 24 amino acid leader sequence (Fig. 1).
KeywordsCatheter HPLC Iodine Angiotensin Dehydration
Unable to display preview. Download preview PDF.
- Currie MG, Geller DM, Cole BR, Siegel NR, Fok KF, Adams SP, Eubanks SR, Galluppi GR, Needleman P (1984 a) Purification and sequence analysis of bioactive atrial pep¬tides (atriopeptins). Science. 223: 67–69Google Scholar
- Currie MG, Sukin D, Geller DM, Cole BR, Needleman P (1984 b) Atriopeptin release from the isolated perfused rabbit heart. Biochem Biophys Res Commun. 124: 711–717Google Scholar
- Currie MG, Geller DM, Cole BR, Needleman P (1984 c) Proteolytic activation of a bioactive cardiac peptide by in vitro trypsin cleavage. Proc Natl Acad Sei USA. 81: 1230–1233Google Scholar
- Lang RE, Tholken H, Ganten D, Luft FC, Rushoaho H, Unger T (1985 a) Atrial natriuretic factor - a circulating hormone stimulated by volume loading. Nature. 314: 264–266Google Scholar
- Lang RE, Unger T, Ganten D, Weil J, Bidlingmaier F, Dohlemann D (1985 b) Alpha-atrial natriuretic peptide concentrations in plasma of children with congenital heart and pulmonary diseases. Br Med J. 291: 1241Google Scholar
- Michener ML, Gierse JK, Seetharam R, Fok KF, Olins PO, Mai MS, Needleman P (1986) Proteolytic processing of atriopeptin prohormone in vivo and in vitro (to be published )Google Scholar
- Nakayama K, Ohkubo H, Hirose T, Inayama S, Nakanishi S (1984) mRNA sequence for human cardiodilantin - atrial natriuretic factor precursor and regulation of precursor mRNA in rat atria. Nature. 310: 699–701Google Scholar
- Sugawara A, Nakao K, Morii N, Sakamoto M, Suda M, Shimokura M, Kiso Y, Kihara M, Yamori Y, Nishimura K, Soneda J, Toshihiko B, Imura H (1985) Alpha-human atrial natriuretic polypeptide is released from the heart and circulates in the body. Biochem Biophys Res Commun. 129: 439–446PubMedCrossRefGoogle Scholar
- Tikkanen I, Metsarinne K, Fyhrquist F, Leidenius R (1985 a) Plasma atrial natriuretic peptide in cardiac disease and during infusion in healthy volunteers. Lancet. 11: 66–69Google Scholar
- Tikkanen I, Metsarinne K, Fyhrquist F (1985 b) Atrial natriuretic peptide in paroxysmal supraventricular tachycardia. Lancet. 11: 40–41Google Scholar
- Wakitani K, Cole BR, Geller DM, Currie MG, Adams SP, Fok KF, Needleman P (1985 a) Atriopeptins - correlation between renal vasodilation and natriuresis. Am J Physiol. 249: F49–F53Google Scholar
- Wakitani K, Oshima T, Loewy AP, Holmberg SW, Cole BR, Adams SP, Fok KF, Currie MG, Needleman P (1985 b) Comparative vascular pharmacology of the atriopeptins. Circ Res. 56: 621–627Google Scholar