Effects of Pantethine on Lipid Metabolism in Isolated Rat Hepatocytes

  • M. Galli Kienle
  • G. Cighetti
  • M. Del Puppo
  • R. Paroni
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Pantethine is the disulfide form of pantetheine the natural precursor of coenzyme A and of the prosthetic group of fatty acid synthetase. Interest in pantethine has increased recently as its lipid-lowering effects were demonstrated in laboratory animals (Shi-nomiya et al. 1980; Tomikawa et al. 1982) and in humans (Maggi et al. 1982; Avogaro et al. 1983; Gaddi et al. 1984). Since the mechanism of these effects is still unknown, we studied the modifications induced by pantethine on lipid metabolism in rat hepatocytes using either radioactive mevalonolactone (MVL) or acetate as the labeled substrate.


Fatty Acid Synthesis Acetate Concentration Fatty Acid Synthetase Disulfide Form Label Acetate 
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  1. Arebalo RE, Tormanen CD, Hardgrave JE, Noland BJ, Scallen TJ (1982) In vivo regulation of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase: immunotitration of the enzyme after short-term mevalonate or cholesterol feeding. Proc Natl Acad Sci USA 79:51–55PubMedCrossRefGoogle Scholar
  2. Avogaro P, Bittolo Bon G, Fusello M (1983) Effect of pantethine on lipids lipoproteins and apoli-poproteins in man. Curr Ther Res 33:488–493Google Scholar
  3. Cighetti G, Galli G, Galli Kienle M (1983) A simple model for studies on the regulation of cholesterol synthesis using freshly isolated hepatocytes. Eur J Biochem 133:573–578PubMedCrossRefGoogle Scholar
  4. Cighetti G, Del Puppo M, Paroni R, Galli G, Galli Kienle M (1986) Effects of pantethine on cholesterol synthesis from mevalonate in isolated rat hepatocytes. Atherosclerosis 60:67–77PubMedCrossRefGoogle Scholar
  5. Edwards PA, Lan S, Tanaka RD, Fogelman AM (1983) Mevalonolactone inhibits the rate of synthesis and enhances the rate of degradation of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase in rat hepatocytes. J Biol Chem 258:7272–7275PubMedGoogle Scholar
  6. Gaddi A, Descovich GC, Noseda G, Fragiacomo C, Colombo L, Craveri A, Montanari G, Sirtori CR (1984) Controlled evaluation of pantethine a natural hypolipidemic compound in patients with different forms of hyperlipoproteinemia. Atherosclerosis 50:73–83PubMedCrossRefGoogle Scholar
  7. Jenke HS, Löwel M, Berndt J (1981) In vivo effect of cholesterol feeding on the short term regulation of hepatic hydroxymethylglutaryl coenzyme A reductase during the diurnal cycle. J Biol Chem 256:9622–9625PubMedGoogle Scholar
  8. Maggi GC, Donati C, Criscuoli G (1982) Pantethine a physiological lipomodulating agent in the treatment of hyperlipidemias. Curr Ther Res 32:380–386Google Scholar
  9. Shinomiya M, Matsuoka N, Shirai K, Morisaki N, Sasaki N, Murano S, Saito Y, Kumagai A (1980) Effect of pantethine on cholesterol ester metabolism in rat arterial wall. Atherosclerosis 36: 75–80PubMedCrossRefGoogle Scholar
  10. Tomikawa M, Nagayasn T, Towara K, Kameda K, Abiko Y (1982) Effect of pantethine on lipoprotein profiles and HDL subfractions in experimentally hypercholesterolemic rabbits. Atherosclerosis 41:267–277PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • M. Galli Kienle
  • G. Cighetti
  • M. Del Puppo
  • R. Paroni
    • 1
  1. 1.Department of Medical Chemistry and BiochemistryUniversity of MilanMilanItaly

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