Analysis of the neu-Encoded Protein and Its Mechanism of Oncogenic Activation
The search for oncogenes and their encoded proteins has involved a variety of experimental procedures and reagents. In our own laboratory, the procedure of gene transfer, also known as transfection, has been used extensively in an attempt to deter oncogenes residing in a variety of tumor DNA’s. These DNA’s have been applied via the Graham-van der Eb calcium phosphate procedure to monolayers of NIH3T3 cells with the hope that some of the transfected cells would respond to donor transforming sequences by demonstrating an altered growth pattern in monolayer. Indeed in this fashion, we and others were able to demonstrate the presence of oncogenes in the DNA’s of a variety of tumor cell types (Varmus 1984).
KeywordsEthyl Agar Tyrosine Recombination Cysteine
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