Analysis of the neu-Encoded Protein and Its Mechanism of Oncogenic Activation

  • C. I. Bargmann
  • D. F. Stern
  • J. Drebin
  • A. Schechter
  • R. A. Weinberg
Conference paper
Part of the Colloquium der Gesellschaft für Biologische Chemie 10.–12. April 1986 in Mosbach/Baden book series (MOSBACH, volume 37)

Abstract

The search for oncogenes and their encoded proteins has involved a variety of experimental procedures and reagents. In our own laboratory, the procedure of gene transfer, also known as transfection, has been used extensively in an attempt to deter oncogenes residing in a variety of tumor DNA’s. These DNA’s have been applied via the Graham-van der Eb calcium phosphate procedure to monolayers of NIH3T3 cells with the hope that some of the transfected cells would respond to donor transforming sequences by demonstrating an altered growth pattern in monolayer. Indeed in this fashion, we and others were able to demonstrate the presence of oncogenes in the DNA’s of a variety of tumor cell types (Varmus 1984).

Keywords

Ethyl Agar Tyrosine Recombination Cysteine 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • C. I. Bargmann
    • 1
  • D. F. Stern
    • 1
  • J. Drebin
    • 2
  • A. Schechter
    • 1
  • R. A. Weinberg
    • 1
  1. 1.Whitehead Institute for Biomedical Research and M.I.T. Department of BiologyNine Cambridge CenterCambridgeUSA
  2. 2.Department of PathologyHarvard Medical SchoolUSA

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