Association of the Postsynaptic 43K Protein with Sites of Clustered and Diffusely-Distributed Acetylcholine Receptors

  • Stanley C. Froehner
  • William J. LaRochelle
  • Amy A. Murnane
Conference paper
Part of the NATO ASI Series book series (volume 3)

Abstract

The formation of the rat neuromuscular junction is a complicated, multistep process that is initiated when the growing nerve first makes contact with embryonic muscle (1). One of the first and most important changes that occurs is an alteration in the distribution of acetylcholine receptors (AChR). Diffusely-distributed receptors become clustered at sites immediately adjacent to the nerve terminal. In adult muscle, receptors are located almost exclusively at synaptic sites at a density of 8–10,000 receptors per square micron of membrane surface (2). Since AChR are multisubunit, transmembrane proteins that are inherently capable of diffusion within the plane of the membrane, special mechanisms must exist that anchor receptors at appropriate sites across the synaptic gap from sites of transmitter release.

Keywords

Oligomer Disulfide Acetylcholine Biotin Rhodamine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dennis, M.J. (1981) Ann. Rev. Neurosci, 4, 43–68.PubMedCrossRefGoogle Scholar
  2. 2.
    Matthews-Belinger, J. and Salpeter, M.M. (1978) J. Physiol. 279, 197–213.Google Scholar
  3. 3.
    Froehner, S.C. (1986) Trends In Neurosci. 9, 37–41.CrossRefGoogle Scholar
  4. 4.
    Burden, S.J., Sargent, P.B., and McMahan, U.J. (1979) J. Cell Biol. 82, 412–425.PubMedCrossRefGoogle Scholar
  5. 5.
    Peng, H.B. and. Froehner, S.C. (1985) J. Cell Biol. 100, 1698–1705.PubMedCrossRefGoogle Scholar
  6. 6.
    Changeux, J.-P., Devillers-Thiery, A. and Chemoulli, P. (1984) Science 225, 1335–1345.PubMedCrossRefGoogle Scholar
  7. 7.
    Cartaud, J., Sobel, A., Rousselet, A., Devaux, P.F., and Changeux, J.-P. (1981) J. Cell Biol. 90, 418–426.PubMedCrossRefGoogle Scholar
  8. 8.
    Rousselet, A., Cartaud, J., Devaux, P.F., and Changeux, J.-P. (1982) EMBO J. 1, 439–445.PubMedGoogle Scholar
  9. 9.
    Lo, M.M.S., Garland, P.B., Lamprecht, J., and Barnard, E.A. (1980) FEBS Lett. 111, 407–412.PubMedCrossRefGoogle Scholar
  10. 10.
    Neubig, R.R., Krodel, E.K., Boyd, N.D., and Cohen, J.B. (1977) Proc. Natl. Acad. Sci. 76, 690–694.CrossRefGoogle Scholar
  11. 11.
    Porter, S. and Froehner, S.C. (1983) J. Biol. Chem. 258, 10034–10040.Google Scholar
  12. 12.
    Gysin, R., Wirth, W. and Flanagan, S.D. (1981) J. Biol. Chem. 256, 11373–11376.PubMedGoogle Scholar
  13. 13.
    Gysin, R., Yost, B., and Flanagan, S.D. (1983) Biochemistry 22, 5781–5789.PubMedCrossRefGoogle Scholar
  14. 14.
    Barrantes, F.J., Mieskes, G., and Walliman, T. (1983) Proc. Natl. Acad. Sci. 80, 5440–5444.PubMedCrossRefGoogle Scholar
  15. 15.
    Sealock, R., Wray, B., and Froehner, S.C. (1984) J. Cell Biol. 98, 2239–2244.PubMedCrossRefGoogle Scholar
  16. 16.
    Froehner, S.C. (1984) J. Cell Biol. 99, 88–96.PubMedCrossRefGoogle Scholar
  17. 17.
    Burden, S.J. (1985) Proc. Natl. Acad. Sci. 82, 8270–8273.PubMedCrossRefGoogle Scholar
  18. 18.
    Burden, S.J., DePalma, R.L., and Gottesman, G.S. (1983) Cell 35, 687–692.PubMedCrossRefGoogle Scholar
  19. 19.
    Porter, S. and Froehner, S.C. (1985) Biochemistry 24, 425–432.PubMedCrossRefGoogle Scholar
  20. 20.
    LaRochelle, W.J. and Froehner, S.C. (1986) J. Biol. Chem. 261, 5270–5274.PubMedGoogle Scholar
  21. 21.
    Lindstrom, J., Tzartos, S., Gullick, W., Hockschwender, S., Swanson, L., Sargent, P., Jacob, M., and Montai, M. (1983) Cold Spring Harbor Symp. Quant. Biol. 48, 89–99.PubMedGoogle Scholar
  22. 22.
    Walker, J.H., Boustead, C.M., and Witzemann, V. (1984) EMBO J. 3, 2287–2290.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Stanley C. Froehner
    • 1
  • William J. LaRochelle
    • 1
  • Amy A. Murnane
    • 1
  1. 1.Department of BiochemistryDartmouth Medical SchoolHanoverUSA

Personalised recommendations