Abstract
My group has been especially interested in developing fast reaction techniques that are suitable for making chemical kinetic measurements with receptor-rich membrane vesicles. More recently we have begun to develop techniques for making similar chemical kinetic measurements of receptor function directly on cell surfaces. We are using the information we have already obtained about the mechanism of action of the acetylcholine receptor to calibrate the new methods, and hope that we can then apply the new methods to investigations of many other neuronal receptor proteins, whose study has been hampered by the lack of the equivalent of the electric organ of fish. Most of these receptor proteins exist in very low concentrations and have been characterized so far only by the binding of a specific neurotransmitter. Mechanistic studies have been almost impossible. We are also developing methods to amplify the quantities of such receptor proteins. The Table is an outline of the topics to be considered in this chapter.
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References
Adams, P. (1977) J. Physiol. (London) 268, 291–318.
Aoshima, H., Cash, D.J., and Hess, G.P. (1981) Biochemistry 3467–3474.
Browning, M.D., Huganir, R.L., and Greengard, P. (1985) J. Neurochem. 45, 11–23.
Cash, D.J. and Hess, G.P. (1981) Anal. Biochem. 112, 39–51.
Colquhoun, D. (1981) in Drug Receptors and Their Effectors (Birdsall, N.J.M., Ed.) pp. 107–127, Macmillan, London.
Fambrough, D. (1979) Physiol. Rev. 59, 165–227.
Goldman, Y.E., Hibberd, M.G., and Trentham, D.R. (1984) J. Physiol. (London) 354, 577–604.
Heidmann, T. and Changeux, J.-P. (1984) Proc. Natl. Acad. Sci. USA 81, 1897–1901.
Hess, G.P., Cash, D.J., and Aoshima, H. (1979) Nature 282, 329–331.
Hess, G.P., Aoshima, H., Cash, D.J., and Lenchitz, B. (1981) Proc. Natl. Acad. Sci. USA 78, 1361–1365.
Hess, G.P., Pasquale, E.B., Karpen, J.W., Sachs, A.B., Takeyasu, K., Cash, D.J. (1982) Biochem. Biophys. Res. Commun. 107, 1583–1589.
Hess, G.P., Cash, D.J., and Aoshima, H. (1983) Ann. Rev. Biophys. Bioeng. 12, 443–473.
Hess, G.P., Kolb, H.-A., Sauger, P., Schoffeniels, E., and Schwarze, W. (1984) Proc. Natl. Acad. Sci. USA 81, 5281–5285.
Huganir, R.L., Delcour, A.H., Greengard, P., and Hess, G.P. (1986) Nature 321, 774–776.
Huganir, R.L., Miles, K., and Greengard, P. (1984) Proc. Natl. Acad. Sci. USA 81, 6968–6972.
Huganir, R.L. and Racker, E. (1982) J. Biol. Chem. 257, 9327–9378.
Junge, D. (1981) Nerve and Muscle Excitation Sinauer, Sunderland, MA.
Kaplan, J.N., Forbush, B., and Hoffman, J.F. (1978) Biochemistry 17, 1929–1935.
Karpen, J.W., Sachs, A.B., Cash, D.J., Pasquale, E.B. and, Hess, G.P. (1983) Anal. Biochem. 135, 83–94.
Karpen, J.W. and Hess, G.P. (1986a) Biochemistry 25, 1777–1785
Karpen, J.W. and Hess, G.P. (1986b) Biochemistry 25, 1786–1792
Kuffler, S.W. and Yoshikamo, D. (1975) J. Physiol. (London) 251, 465–482.
Leviton, I.B. (1985) J. Membr. Biol. 87, 177.
McCray, J.A., Herbette, L., Kihara, T., and Trentham, D.R. (1980) Proc. Natl. Acad. Sci. USA 77, 7237–7241.
Moore, H.-P.H. and Raftery, M.A. (1980) Proc. Natl. Acad. Sci. USA 77, 4509–4513.
Neher, E. and Sakmann, B. (1976) Nature 260, 779–802.
Neher, E. and Steinbach, J.H. (1978) J. Physiol. (London) 227, 153–176.
Nerbonne, J.M., Richard, S., Nargeot, J., and Lester, H.A. (1984) Nature (London) 310, 74–76.
Ogden, D.C. and Colquhoun, D. (1985) Proc. Roy. Soc. London, B 225, 329.
Oswald, R.E., Heidmann, T., and Changeux, J.-P. (1983) Biochemistry 22, 3128–3136.
Pasquale, E.B., Takeyasu, K., Udgaonkar, J.B., Cash, D.J., Severski, M., and Hess, G.P. (1983) Biochemistry 22, 5967–5973.
Pillai, V.N.R. (1980) Synthesis 1, 1–26.
Ramseyer, G.O., Morrison, G.H., Aoshima, H., and Hess, G.P. (1981) Anal. Biochem. 115, 34–36.
Sakmann, B., Methfessel, C., Mishina, M., Takahashi, T., Takai, T., Kurasaki, M., Fujida, K. and Numa, S. (1986) Nature 318, 538–542.
Sakmann, B. and Neher, E. (1984) Ann. Rev. Physiol. 46, 455–472.
Schwartz, J.H., Bernier, L., Castellucci, V.F., Pollazzole, M., Saitoh, T., Stapleton, A., and Kandel, E.R. (1983) Cold Spring Harbor Symposium on Quantitative Biology 48, 811–819.
Shiono, S., Takeyasu, K., Udgaonkar, J.B., Delcour, A.H., Fujita, N., and Hess, G.P. (1984) Biochemistry 23, 6889–6893.
Sine, S.M. and Steinbach, J.H. (1984) Biophys. J. 46, 277–284.
Steinbach, J.H. (1980) in The Cell Surface and Neuronal Function (Cotman, C.S., Poste, G. and Nicolson, G.L., Eds.) pp. 119–156, Elsevier/North-Holland Biomedical Press, New York.
Stryer, L. (1975) Biochemistry. W.H. Freeman and Co., San Francisco.
Takeyasu, K., Shiono, S., Udgaonkar, J.B., Fujita, N. and Hess, G.P. (1986) Biochemistry 25, 1770–1776.
Takeyasu, K., Udgaonkar, J.B. and Hess, G.P. (1983) Biochemistry 22, 5973–5978.
Tank, D.W., Huganir, R.L., Greengard, P. and Webb, W.W. (1983) Proc. Natl. Acad. Sci. USA 80, 5129–5133.
Thesleff, S. and Molgo, J. (1984) Neuroscience (Oxford) 9, 1–8.
Walker, J.W. and Hess, G.P. (1986) Biochemistry 25, 1799–1805.
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Hess, G.P. (1986). Regulation of Intercellular Signal Transmission. New Approaches to Chemical Kinetic Measurements. In: Maelicke, A. (eds) Nicotinic Acetylcholine Receptor. NATO ASI Series, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71649-2_14
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DOI: https://doi.org/10.1007/978-3-642-71649-2_14
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