Mammalian Cell Transformation by a Recombinant Murine Retrovirus Containing the Avian Erythroblastosis Virus erbB Gene
Avian erythroblastosis virus (AEV) is a replication-defective retrovirus which induces erythroblastosis, sarcomas and carcinomas in chickens and transforms immature avian erythroid and fibroblastic cells in vitro (Graf and Beug, 1978). The genome of AEV contains two distinct oncogenes, v-erbA and v-erbB (Vennstrttm and Bishop, 1982). V-erbB is a member of the src family of retroviral oncogenes (Yamamoto et. al., 1983). The products of these src -related oncogenes as well as the receptors Tor several growth factors have been slown to exhibit tyrosine-specific kinase activity (Hunter and Cooper, 1985). Recently, it has been found that the v-erbB gene sequence possesses extensive homology with the mammalian epidermal growth factor (EGF) receptor gene (Downward et al., 1984). Analysis of the range of mammalian target cells susceptible to transformation by v-erbB has been impaired by difficulties in introducing this avian virus into mammalian cells. Therefore, we constructed a recombinant v-erbB-containing murine retrovirus which has allowed for more extensive investigation of the function of this EGF receptor-related gene as a transforming or growth-promoting gene in well defined mammalian systems.
KeywordsHematopoietic Cell Hematopoietic Cell Line Poietic Cell Avian Sarcoma Virus Abelson Murine Leukemia
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- Downward J, Yarden Y, Mayes E, Scrace G, Totty N, Stockwell P, Schlessinger J, Waterfield MD (1984) Close similarity of epidermal growth factor receptor and v-erbB oncogene protein sequences. Nature 307: 521: 527Google Scholar
- Kahn P, Adkins B, Beug H, Graf T (1984) Src- and fps-containing avian sarcoma viruses transform chicken erythroid ceTTs. Proc Natl Acad Sci 81: 7122–7126Google Scholar