Aspartyl-tRNA Synthetase-Induced Aspartylation of Proteins: a Fingerprint Approach to Map Accessible Domains in Protein

  • H. Mejdoub
  • D. Kern
  • R. Giege
  • Y. Boulanger
  • J. Reinbolt

Abstract

Aspartic acid can be covalently linked to yeast aspartyl-tRNA synthetase and to other proteins in the absence of tRNA, under conditions where the synthetase activates the amino acid into aspartyl-adenylate, i.e., in the presence of ATP and MgCl2 [1,2]. The aspartyl adenylate is poorly bound to the enzyme.

Keywords

HPLC Serine Lysine Ketone Trypsin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lorber B, Kern D, Giege R, Ebel JP (1982) FEBS Lett 146:59.PubMedCrossRefGoogle Scholar
  2. 2.
    Kern D, Lorber B, Boulanger Y, Giege R (1985) Biochemistry 24:1321.CrossRefGoogle Scholar
  3. 3.
    Amiri I, Mejdoub H, Hounwanou N, Boulanger Y, Reinbolt J (1985) Biochimie 67:607.PubMedCrossRefGoogle Scholar
  4. 4.
    Lorber B, Kern D, Dietrich A, Gangloff J, Ebel JP, Giege R (1983) Biochem Biophys Res Commun 117:259.PubMedCrossRefGoogle Scholar
  5. 5.
    Keith G, Gangloff J, Dirheimer G (1971) Biochimie 53:123.CrossRefGoogle Scholar
  6. 6.
    Dock C, Lorber B, Moras D, Pixa G, Thierry JC, Giege R (1984) Biochimie 66:179.PubMedCrossRefGoogle Scholar
  7. 7.
    Hounwanou N, Boulanger Y, Reinbolt J (1983) Biochimie 65:379.PubMedCrossRefGoogle Scholar
  8. 8.
    Kern D, Gangloff J (1981) Biochemistry 20:538.Google Scholar
  9. 9.
    Giege R, Lorber B, Ebel JP, Moras D, Thierry JC, Jacrot B, Zaccai G (1982) Biochimie 64:357.PubMedCrossRefGoogle Scholar
  10. 10.
    Lorber B, Giege R (1983) FEBS Lett 156:209.PubMedCrossRefGoogle Scholar
  11. 11.
    Igloi GL, von der Haar F, Cramer F (1978) Biochemistry 17:3459.PubMedCrossRefGoogle Scholar
  12. 12.
    Lin SX, Baltzinger M, Remy P (1983) Biochemistry 22:681.PubMedCrossRefGoogle Scholar
  13. 13.
    Hopp T, Woods KR (1981) Proc Natl Acad Sci USA 78:3824.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • H. Mejdoub
    • 1
  • D. Kern
    • 1
  • R. Giege
    • 1
  • Y. Boulanger
    • 1
  • J. Reinbolt
    • 1
  1. 1.Institut de Biologie Moléculaire et Cellulaire du C.N.R.S.Laboratoire de BiochimieStrasbourgFrance

Personalised recommendations