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Immuno-EM of Sepia officinalis hemocyanin

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Invertebrate Oxygen Carriers

Abstract

Negatively stained molecules of Sepia officinalis hemocyanin (Hc) (Mr 3.8×106) have the form of a partly hollow cylinder with 350 A diameter and 170 A height. The circular profiles show material inside the cylinder: the so-called collar which has 5-fold (or possibly 10-fold) symmetry (1). At higher pH values this Hc dissociates into five subunits, each consisting of 2×8 domains (a-h) with average Mr of 50,000 (2). Most probably this dissociation is similar to the dissociation of half Helix pomatia α-Hc molecules, i.e. along helical grooves in the cylinder wall (3).

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References:

  1. Bruggen, E.F.J. van, Wiebenga, E.H. and Gruber, M., J.Mol.Biol. 4, 8–9 (1962).

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  2. Gielens, C., Bosman, F., Préaux, G. and Lontie, R. in Structure and Function of Invertebrate Respiratory Proteins, ed. E.J. Wood, Harwood Academic Publishers, Chur, London, New York, 1983, p. 121–124.

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  3. Siezen, R.J. and Bruggen, E.F.J. van, J.Mol.Biol. 90, 77–89 (1974).

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© 1986 Springer-Verlag Berlin Heidelberg

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Wichertjes, T., Gielens, C., Schutter, W.G., Préaux, G., Lontie, R., van Bruggen, E.F.J. (1986). Immuno-EM of Sepia officinalis hemocyanin. In: Linzen, B. (eds) Invertebrate Oxygen Carriers. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71481-8_39

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  • DOI: https://doi.org/10.1007/978-3-642-71481-8_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-16943-7

  • Online ISBN: 978-3-642-71481-8

  • eBook Packages: Springer Book Archive

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