Abstract
Epididymal proteins obtained by shaking slices of tissue or from tissue minces (Gupta et al., 1974; Arora et al., 1975; Bose et al., 1975; Vreeburg. 1975; Huang and Johnson, 1976) necessarily contain proteins from blood and lymph in addition to those from the epididymal lumen. Bloodless micropuncture of the tubules provides uncontaminated samples with lower protein concentrations than blood (Table 18) and a variety of different proteins separable by gel electrophoresis (Koskimies and Kormano, 1975; Turner, 1979; Turner and Plesums, 1979; Hamilton, 1981b; Wong et al., 1981; Olson and Hinton, 1985) that are not present in serum.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aafjes JH and Vreeburg JTM (1972) Distribution of 5α-dihydrotcstosterone in the epididymis of bull and boar, and its concentration in rat epididymis after ligation of efferent testicular ducts, castration and unilateral gonadectomy. J Endocr 53: 85–93
Aarons D, Robinson R, Richardson R and Poirier GR (1985) Competition between seminal and exogenous proteinase inhibitor for sites on murine epididymal sperm. Contraception 31: 177–184
Abe K. Takano II and Ito T (1982a) Response of the epididymal duct in the corpus epididymis to efferent or epididymal duct ligation in the mouse. J Reprod Fert 64: 69–72
Abe K. Takano H and Ito T (1982b) Appearance of peculiar epithelial cells in the epididymal duct of the mouse ligated epididymis. Biol Reprod 26: 501–509
Abe K. Takano H and Ito T (1983) Response of the epididymal duct to the temporary depletion of spermatozoa induced by testicular irradiation in mice. Anat Record 207: 17–24
Abe K, Takano II and Ito T (1984) Appearance of peculair multivesicular bodies in the principal cells of the epididymal duct after efferent duct cutting in the mouse. Arch Histol Japanicum 47: 121–135
Abrcsca P., Lombardi G, de Rosa M, Quagliozzo L. Guardiola J and Metafora S (1985) Identification and preliminary characterization of a sperm-binding protein in normal human semen. J Reprod Fert 73: 71–77
Acott TS and Hoskins DD (1981) Bovine sperm forward motility protein: binding to epididymal spermatozoa. Biol Reprod 24: 234–240
Acott TS and Carr DW (1984) Inhibition of bovine spermatozoa by cauda epididymal fluid: II Interaction of pH and a quiescence factor. Biol Reprod 30: 926–935
Adrian TE. Gu J. Allen JM, Tatemoto K. Polak JM and Bloom SR (1984) Neuropeptide Y in the human male genital tract. Life Sci 35: 2643–2648
Aisen P and Litowsky I (1980) Iron transport and storage proteins. Ann Rev Biochem 49: 357–393
Albert Z. Orlonwski J, Orlowsky M and Szenczuk A (1964) Histochemical and Biochemical investigations of gamma-glutamyl transpeptidase in the tissues of man and laboratory rodents. Acta Histochem 18: 78–89
Alm P. Alumels J, Hakansen and Sundler F (1977) Peptidergic (vasoactive intestinal peptide) nerves in the genito-urinary tract. Neuroscience 2: 751–754
Alm P. Alumels J. Hakansen R. Owman C, Sjoberg NO. Sundler F and Walles B (1980) Origin and distribution of VIP (vasoactive intestinal peptide)-nerves in the genitourinary tract. Cell Tiss Res 205: 337–347
Al-Timini DJ and Dormandy TL (1977) The inhibition of lipid autoxidation by human caeruloplasmin. Biochem J 168: 283–288
Alumot F., Lensky Y and Schindler H (1971) Separation of proteins in the epididymal fluid of the ram. J Reprod Fert 25: 349–353
Amann RP, Killian GJ and Benton AW (1973) Differences in the electrophoretic characteristics of bovine rete testis fluid and plasma from the Cauda epididymidis. J Reprod Fert 35: 321–330
Amir D and Lavon U (1976) Changes in total nitrogen, lipoproteins and amino acids in epididymal and ejaculated spermatozoa from bulls treated orally with ethylene dibromide. J Reprod Fert 47: 73–76
Anderson DJ and Tartar TH (1982) Immunosuppressive effects of mouse seminal plasma components in vivo and in vitro. J Immunol 128: 535–539
Aonuma S. Mayumi T. Suzuki K. Noguchi T. Iwai I and Okabe M (1973) Studies on sperm capacitation. I. The relationship between a guinea-pig sperm-coating antigen and a sperm capacitation phenomenon. J Reprod Fert 35: 425–432
Aragona C and Friesen HG (1975) Specific prolactin binding sites in the prostate and testis of rats. Endocrinology 97: 677–684
Arora R. Dinakar N and Prasad MRN (1975) Biochemical changes in the spermatozoa and luminal contents of different regions of the epididymis of the Rhesus monkey, Macaca mulatto. Contraception 11: 689–700
Attramadal A, Bardin CW, Gunsalas GL, Musto NA and Hansson V (1981) Immunocytochemical localization of androgen binding protein in rat Sertoli and epididymal cells. Biol Reprod 25: 983–988
Back DJ, Glover TD, Shenton JC and Boyd CP (1977) Some effects of cyproterone acetate on the reproductive physiology of the male rat. J Reprod Fert 49: 237–43
Barber J (1982) The control of membrane organization by electrostatic forces (review). Bioscience Reports 2: 1–13
Barnes D and Sato G (1980) Methods for growth of cultured cells in serum-free medium. Anal Biochem 102: 255–270
Bartsch G, Oberholzer M. Holliger O. Weber J. Weber A and Rohr IIP (1978) Stereology: a new quantitative method to study epididymal function. Androl 10: 31–42
Bayard F. Duguet A. Mazzuca M and Faye JC (1981) Study of a glycoprotein produced by the rat epididymis. In: Reproductive Processes and Contraception. Ed KW McKerns. Plenum Pub Co. pp
Bearer EL and Friend DS (1980) Anionic lipid domains: correlation with functional topography in a mammalian cell membrane. Proc Natn Acad Sci US 77: 6601–6605
Bennett G (1970) Migration of glycoprotein from Golgi apparatus to cell coat in the columnar cells of the duodenal epithelium. J Cell Biol 45: 668–673
Bennett G and Leblond CP (1970) Formation of cell coat material for the whole surface of columnar cells in the rat small intestine, as visualised by radioautography with L-fucose-AH. J Cell Biol 46: 409–416
Bennett G. Leblond CP and Haddad A (1974) Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labelled fucose injection into rats. J Cell Biol 60: 258–284
Bernals A. Torres J. Reyes A and Rosado A (1980) Presence and regional distribution of sialyltransferase in the epididymis of the rat. Biol Reprod 23: 290–293
Bigelow CC (1967) On the average hydrophobicity of proteins and the relation between it and protein structure. J Theoret Biol 16: 187–211
Blank M, Soo L and Britten.IS (1976) Adsorption of albumin on rabbit sperm membranes. J Memb Biol 29: 401–409
Blaquier JA (1973) An in vivo action of androgens on protein synthesis by epididymal tubules maintained in organ culture. Biochem Biophys Res Commun 52: 1177–1183
Blaquier JA (1975) The influence of androgens on protein synthesis by cultured rat epididymal tubules. Acta Endocr 79: 403–416
Blaquier JA and Breger D (1974) The in vitro effects of androgens on RNA synthesis by cultured rat epididymal tubules. Endocrine Res Commun 1: 247–260
Blaquier JA and Breger J (1975) The in vitro effects of androgens on RNA synthesis by cultured rat epididymal tubules. Endocr Res Commun 1: 247–260
Blaquier JA. Cameo MS and Calandra R (1974) Studies on the correlation between androgen binding and activation of rat epididymal tubules in culture. In: Hormone Binding and Target Cell Activation in the Testis. Eds ML Dufau and AR Means. Current Topics in Endocrinology volume 1, Plenum Press. New York, pp 329–341
Blaquier JA. Breger D. Cameo MS and Calandra R (1975) The activation of cultured epididymal tubules by androgens. J Steroid Biochem 6: 573–576
Blaschuk OW and Fritz IB (1984) Isoelectric forms of clusterin isolated from ram rete testis fluid and from secretions of primary cultures of ram and rat Sertoli cell-enriched preparations. Can J Biochem Cell Biol 62: 456–461
Blaschuk O. Brudzy K and Fritz IB (1983) Purification and characterization of a cell-aggregating factor (Clusterin), the major glycoprotein in ram rete testis fluid. J Biol Chem 258: 7714–7720
Bose AR and Kar AB (1968) Distribution of sialic acid in the genital organs of male rhesus monkeys: effect of castration and replacement therapy. Current Sci (India) 37: 168–169
Bose AR. Kar AB and Dasgupta PR (1966) Sialic acid in the genital organs of the male rat. Current Sci (India) 35: 336–337
Bose TK, Prasad MRN and Moudgal NR (1975) Changing patterns of silaic acid in the spermatozoa and luminal plasma of the epididymis and vas deferens of the hamster. Mesocriceius auratus (Warterhouse). Ind J exp Biol 13: 8–11
Brooks DE (1973) Epididymal and testicular temperature in the unrestrained conscious rat. J Reprod Fert 35: 157–160
Brooks DE (1977) The androgenic control of the composition of the rat epididymis determined by efferent duct ligation or castration. J Reprod Fert 49: 383–385
Brooks DE (1979) Carbohydrate metabolism in the rat epididymis: evidence that glucose is taken up by tissue slices and isolated cells by a process of facilitated transport Biol Reprod 21: 19–26
Brooks DE (1981) Secretion of proteins and glycoproteins by the rat epididymis: regional differences, androgen-dependence, and effects of protease inhibitors, procaine, and tunicamycin. Biol Reprod 25: 1099–1117
Brooks DE (1982) Purification of rat epididymal proteins “D” and “E”, demonstration of shared immunological determinants, and identification of regional synthesis and secretion. Int J Androl 5: 513–524
Brooks DE (1983a) Effect of androgens on protein synthesis and secretion in various regions of the rat epididymis, as analyzed by two-dimensional gel electrophoresis. Mol Cell Endocrinol 29: 255–270
Brooks DE (1983b) Influence of incubation conditions, tunicamycin and castration on incorporation of (3H)mannose and (3H)fucose into rat epididymal glycoproteins in vitro. J Reprod Fert 67: 97–105
Brooks DE (1983c) Epididymal protein secretion. In: The Sperm Cell. Ed J Andre. Martinus Nijhoff, The Hague, pp 175–178
Brooks DE (1983d) Selective binding of specific rat epididymal secretory proteins to spermatozoa and erythrocytes. Gamete Res 7: 367–376
Brooks DE (1984) Protein secretion by the rat epididymis can be selectively modified in vitro by local anaesthetics, glucose deprivation, dinitrophenol. ouabain, and ionophores. J Androl 5: 351–360
Brooks DE and Higgins SJ (1980) Characterization and androgen-dependence of proteins associated wih luminal fluid and spermatozoa in the rat epididymis. J Reprod Fert 59: 363–375
Brooks DE and Tiver K (1983) Localization of epididymal secretory proteins on rat spermatozoa. J Reprod Fert 69: 651–657
Brooks DE and Tiver K (1984) Analysis of surface proteins of rat spermatozoa during epididymal transit and identification of antigens common to spermatozoa, rete testis fluid and cauda epididymal plasma. J Reprod Fert 71: 249–257
Brooks DE. Means AR. Wright EJ, Singh SP and Tiver KK (1986) Molecular cloning of the cDNA for two major androgen-dependent secretory proteins of 18.5 kilodaltons synthesized by the rat epididymis. J Biol Chem 26: 4965–4961
Brown KD. Blakeley DM, Henville A and Setchell BP (1982a) Rete testis fluid contains a growth factor for cultured fibroblasts. Biochem Biophys Res Commun 105: 391–397
Brown KD. Henville A and Setchell BP (1982b) A growth factor in rete testis fluid. Ann New York Acad Sci US 383: 428–429
Brown CR. von Glos KI and Jones R (1983) Changes in plasma membrane glycoproteins of rat spermatozoa during maturation in the epididymis. J Cell Biol 96: 256–264
Byers SW, Qasba PK and Paulson HL (1984) Immuno cytochemical localization of α-lactalbumin in the male reproductive tract. Biol Reprod 30: 171–177
Byers SW, Musto NA and Dym M (1985) Culture of ciliated and nonciliated cells from rat ductuli efferentes. J Androl 6: 271–278.
Cameo MS and Blaquier JA (1976) Androgen-controlled specific proteins in rat epididymis. J Endocr 69: 47–55
Chapdelaine P. Tremblay RR. Dube RR. St-Yves C and Mailhot J (1981) Origin of maltase and variations in infertile men. Archs Androl 1: 61–68
Caharrcau EH. Baldi A. Blaquier J and Wasserman G (1970) Studies on the mechanism of action of testosterone and dihydrotestosterone uptake and effects on RNA metabolism in castrated rats. Acta Endocr Panam 1: 39–48
Chaudhury DP and Majumder GC (1983) Evidence for the reconstitution of motility by epididymal plasma-protein factors(s) in immotile washed spermatozoa from goat cauda epididymis. Andrologia 15: 495–497
Chinoy NJ and Sanjecvan AG (1980) Effects of an intra-epididymal and intra-scrotal copper device on rat spermatozoa. Int J Androl 3: 719–737
Chulavatnatol M (1982) Motility initiation of quiescent spermatozoa from rat caudal epididymis: effects of pH, viscosity, osmolality and inhibitors. Int J Androl 5: 425–436
Courtens JL, Rozinek J and Fournier-Delpech S (1982) Binding of epididymal proteins to the spermatozoa of ram. Andrologia 14: 509–514
Cuasnicii PS. Gonzalez Echeverria F, Piazza A and Blaquier JA (1984) Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa. J Reprod Fert 70: 541–547
Cushman DW and Cheung IIS (1971) Concentrations of angiotensin converting enzyme in tissues of the rat. Biochem Biophys Acta 250: 261–265
Cooper TG and Waites GMH (1979) Investigation by luminal perfusion of the transfer of compounds into the epididymis of the anaesthetized rat. J Reprod Fert 56: 159–164
Cooper TG and Yeung CH (1980) Epithelial structure of the rat cauda epididymidis after luminal perfusion. Int J Androl 3: 367–374
Dacheux JL (1980) An in vitro luminal perfusion technique to study epididymal secretion. IRCS Med Sci 8: 137
Dacheux JL and Voglmayr JK (1983) Sequence of sperm cell surface differentiation and its relationship to exogenous fluid proteins in the ram epididymis. Biol Reprod 29: 1033–1046
Dacheux JL. Paquignon M and Combamous Y (1983) Head-to head agglutination of ram and boar epididymal spermatozoa and evidence for an epididymal antagglutinin. J Reprod Fert 67: 181–189
Dadoune JP (1981) Structural and metabolic findings on epididymal cells. In: Epididymis and Fertility: Biology and Pathology. Eds C Bollack and A Clavert, S Karger. London. Progr Reprod Biol 8: 34–47
D’Agnostino A. Jones R. White R and Parker MG (1980) Androgenic regulation of messenger RNA in rat epididymis. Biochem J 190: 505–512
Danzo BJ, Filer BC and Orgebin-Crist MC (1976) Approaches to fertility regulation through an understanding of the molecular control of epididymal function. In: Sperm Action. Progress in Reproductive Biology Volume 1. Eds PO Hermite. M L’Hermite and J Schirren. Karger. Basel, pp 74–88
Danzo BJ. Cooper TG and Orgebin-Crist MC (1977) Androgen binding protein (ABP) in fluid collected from the rete testis and cauda epididymidis of sexually mature and immature rabbits and observations on morphological changes in the epididymis following ligation of the ductuli efferentes. Biol Reprod 17: 64–77
Davis BK and Gergely AF (1979) Studies on the mechanism of capacitation: changes in plasma membrane proteins of rat spermatozoa during incubation. Biochem Biophys Res Commun 88: 613–618
DcLap I.V. Tate SS and Meister A (1977) y-glutamyl transpeptidase and related enzyme activities in the reproductive system of the mac rat. Life Sci 20: 673–680
del Rio AG (1979) Macromolccular secretion into v arious segments of the guinea pig epididymis. Archs Androl 3: 231–237
del Rio AG, DeSanchez LZ and Sirena A (1984) Evaluation of epididymal function through specific protein on spermatozoa. Archs Androl 12: 195–196
del Villano BC, Lerner RA, Kennel SJ and Elder JH (1977) Expression of an oncornavirus gene product in the mouse genital tract. In: The Testis in Normal and Infertile Men. Eds P Troen and HR Nankin, Raven Press, New York pp 173–183
Devine KM and Carroll J (198$) Peptide synthesis by the murine epididymis. Biochem Soc Trans 13: 506–507
Dostäl J and Veselsky L (1972) Proteins in the seminal plasma and the accessory sexual gland iluids of the boar. J Reprod Fert 30: 255–267
Doit HM. Harrison RAP and Foster GCA (1979) The maintenance of motility and the surface properties of epididymal spermatozoa from bull, rabbit and ram in homolgous seminal and epididymal plasma. J Reprod Fert 55: 113–124
Dravland E and Joshi MS (1981) Sperm-coating antigens secreted by the epididymis and seminal vesicle of the rat. Biol Reprod 25: 649–658
Durliat M and Vendrely C (1972) Etude de Taction des androgenes sur Incorporation de cytidinc dans les organes du tractus genital dc la souris male. 1. Influence de la castration et de l’implaniation d’androgene chez les castrats. Comptes Rendus Soc de Biol 166: 1578–1585
Emery T (1980) Iron deprivation as a biological defence mechanism. Nature (Lond) 287: 776–777
Ellis DH, Hartman T and Moore (1985) Maturation and function of the hamster spermatozoon probed with monoclonal antibodies. J Reprod Immunol 7: 299–314
Elson SD, Browne CA and Thorburn GD (1984) Identification of epidermal growth factor-like activity in human male reproductive tissues and fluids. J Clin Endocr 58: 589–594
Esponda P and Bedford JM (1985) Epididymal fluid macromolecules do not at as auto- or alloantigens. J Androl 6: 359–364
Fain-Maurel MA, Dadoune JP and Alfons MF (1981) High-resolution autoradiography of newly formed proteins in the epididymis after incorporation of tritiated amino acids. Archs Androl 6: 249–266
Fain-Maurel MA. Dadoune JP and Jauzein-Leau F (1983) Protein secretion by the principal cells of mouse epididymis as evidenced by in vitro incorporation of tritiated leucine. Reprod Nutr Develop 23: 175–182
Fayc JC, Duguet L, Mazzuca M and Bayard F (1980) Purification, radioimmunoassay, and immunohistochemical localization of a glycoprotein produced by the rat epididymis. Biol Reprod 23: 423–32
Feig LA. Klagsbrun and Bellve AR (1983) Mitogenic poplypeptide of the mammalian seminiferous epithelium: biochemical characterization and partial purification. J Cell Biol 97: 1435–1443
Feuchter FA. Vernon RB and Eddy EM (1981) Analysis of the sperm surface with monoclonal antibodies: topographically restricted antigens appearing in the epididymis. Biol Reprod 24: 1099–1110
Fleischer B, Fleischer S and Ozawa H (1969) Isolation and characterization of Golgi membranes from bovine liver. J Cell Biol 43: 59–79
Flickinger CJ (1979) Synthesis, transport and secretion of protein in the initial segment of the mouse epididymis as studied by electrón microsocpe radioautography. Biol Reprod 20 1015–1030
Flickinger CJ (1981) Regional differences in synthesis, intracellular transport, and secretion of protein in the mouse epididymis. Biol Reprod 25: 871–883
Flickinger CJ (1983) Synthesis and secretion of glycoprotein by the epididymal epithelium. J And rol 4: 157–161
Flickinger CJ (1985) Radioautographic analysis of the secretory pathway for glycoproteins in principal cells of the mouse epididymis exposed to (3H)fucose. Biol Reprod 32: 377–389
Flickinger CJ. Wilson KM and Gray HA (1984) The secretory pathway in the mouse epididymis as shown by electron microscope radioautography of principal cells exposed to monensin. Anat Rec 210: 435–448
Fournier S (1966) Repartition de l’acide sialique dans I’appareil genital rat Wistar d’adulte normal et castré. Comptes Rendus Soc de Biol 160: 1087–1090
Fournier-Delpech S. Bayard F and Boulard C (1973a) Contribution á 1’ctudc de la maturation du sperme. Etude d’une proteine acide de l’épididyme chez le rat. Dependence androgene, relation avee l’acidc sialique. CR Soc de Biol 167: 1989–1996
Fournier-Delpech S, Danzo BJ and Orgebin-Crist MC (1977) Extraction of concanavalin A affinity material from rat testicular and epididymal spermatozoa. Ann biol anim Bioch Biophys 17: 207–213
Fournier-Delpech S. Hamamah S. Colas G and Courot M (1983a) Aquisition of zona binding structures by ram spermatozoa during epididymal passage. In: The Sperm Cell. Ed J Andre. Martinus-Nijhoff, The Hague, pp 103–106.
Fritz IB. Burdyzy K. Setchell BP and Blaschuk D (1983) Ram rete testis fluid contains a protein (Clusterin) which influences cell-cell interactions in vitro. Biol Reprod 28: 1173–1188
Fox N. Damjanov I, Knowles BB and Solter D (1982) Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm. Exp Cell Res 137: 485–486
Garberi JC. Kohane AC, Cameo MS and Blaquier JA (1979) Isolation and characterization of specific rat epididymal proteins. Mol Cell Endocr 13: 73–82
Gaunt SJ (1982) A 28K-dalton cell surface autoantigen of spermatogenesis: characterization using a monoclonal antibody. Develop Biol 89: 92–100
Gaunt SJ. Brown CR and Jones R (1983) Identification of mobile and fixed antogens on the plasma membrane of rat spermatozoa using monoclonal antibodies. Exp Cell Res 144: 275–284
Gingell D (1976) Electrostatic control of membrane permeability via intramembranous particle aggregation. In: Mammalian Cell Membranes Vol 1 General Concepts. Eds GA Jamicson and DM Robinson. Butterworths. London, pp 198–223
Glenner GG. Folk JE and McMillan PJ (1962) Histochemical demonstration of a gammaglutamyl transpeptidase-like activity. J Histo Cytochem 10: 481–489
Glucksmann A. Howards A and Pele SR (1955) Incoportaitn of 35S-DL-methionine in mouse tissue as indicated by autoradiography. 1. Testis, epididymis and seminal vesicle. J Anat 89: 13–18
Goldbarg JA, Friedman OM, Pinera EP, Smith EE, Chatterji R, Stein EH and Rutenberg AM (1960) The colorimetric determination of γ-glutamyltranspeptidase with synthetic substrate. Arch Biochem Biophys 91: 61–70
González Echeverría FM. Cuasnicú PS and Blaquier JA (1982) Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa. J Reprod Fert 64: 1–7
Guerin JF, Rollet J. Perrin P, Menezo Y, Orgiazzi A and Czyba JC (1981) Enzymes in the seminal plasma from azoospermic men: correlation with the origin of their azoospermia. Fert Stcril 36: 368–372
Gunn RMC (1936) Fertility in sheep. Artificial production of seminal ejaculation and the characteristics of the spermatozoa contained therein. CSIRO Bulletin No. 94. Commonwealth of Australia
Gupta G. Rajalakshmi M, Prasad MRN and Moudgal NR (1974) Alteration in epididymal function and its relation to maturation of spematozoa. Andrologia 6: 35–44
Hales BF. Hachey C and Robaire B (1980) The presence and longitudinal distribution of the glutathione S-transferases in rat epididymis and vas deferens. Biochem J 189: 135–142
Hamilton DW (1981a) Future trends in cell biological research on the male reproductive system. In: Goals in Male Reproductive Research. Eds S Boyarsky and K Polakoski. Pergamon Press, Oxford, pp 19–27
Hamilton DW (1981b) Evidence for a-lactalbumin-like activity in reproductive tract fluids of the male rat. Biol Reprod 25: 385–392
Hamilton DW, Wenstrom JC and Baker JB (1986) Membrane glycoproteins from spermatozoa: partial characterization of an integral Mr = ∽ 24,000 molecule from rat spermatozoa that is glycosylated during epididymal maturation. Biol Reprod (in press)
Harrison RAP, Dott HM and Foster GC (1978) Effect of ionic strength, serum albumin and other macromolecules on the maintenance of motility and the surface of mammalian spermatozoa in a simple medium. J Reprod Fert 52: 65–73
Harrison RAP, Dott HM and Foster GC (1982) Bovine scrum albumin, sperm motility, and the “dilution effect”. J exp Zool 222: 81–88
Higgins SJ, Brooks DE and Fuller FM (1981) Isolation of cells from rat seminal vesicles and epididymis and their use in studying androgen action. Mol Cell Endocr 23: 207–223
Hinton BT and Howards SS (1982a) Rat testis and epididymis can transport (3H)3-O-methyl-D-glucose, (3H)inositol and (3H)α-aminoisobutyric acid across its epithelia in vivo. Biol Reprod 27: 1181–1189
Hoffer AP. Hamilton DW and Faweett DW (1973) The ultrastructure of the principal cells and intraepithelial leucocytes in the initial segment of the rat epididymis. Anal Rec 175: 169–202
Hoffmann DS and Killian GJ (1981) Isolation of epithelial cells from the corpus epididymidis and analysis for glycerylphosphorylcholine. sialic acid and protein. J exp Zool 217: 93–102
Hohlbrugger G, Schweisfurth H and Dahlheim H (1982) Angiotensin I converting enzyme in rat testis, epididymis and vas deferens under different conditions. J Reprod Fert 65: 97–103
Huang HFS and Johnson AD (1975) Amino acid composition of epididymal plasma of mouse, rat. rabbit and sheep. Comp Biochem Physiol 50B: 359–362
Huggenvik J and Griswold MD (1981) Retinol binding protein in rat testicular cells. J Reprod Fert 61: 403–408
Hunter AG and Schellpfeffer DA (1981) Concentrations of sperm, protein and a sperm membrane glycoprotein within boar epididymal luminal fluids. J Anim Sci 52: 575–579
Hunter RUF. Holtz W and Hermann II (1978) Stabilizing role of epididymal plasma in relation to the capacitation time of boar spermatozoa. Anim Reprod Sci 1: 161–166
Irwin M. Nicholson N, Haywood JT and Poirier GR (1983) Immunological localization of a murine seminal vesicle proteinase inhibitor. Biol Reprod 28: 1201–1206
Iusem ND, de Larminat MA, Tezon JG. Blaquier JA and Belocopitow E (1984) Androgen dependence of protein.V-glycosylation in rat epididymis. Endocrinology 114: 1448–1453
Jaiswal A. Joshi P, Kumar MV, Panda JN and Singh I.N (1984) Angiotensin converting enzyme in the tesis and epididymis of mammals. Andrologia 16: 410–416
Jaiswal AK. Panda JN, Vijaykumar M and Joshi P (1985) Androgen dependence of testicular and epididymal angiotensin converting enzyme. Andrologia 17: 92–97
Jegou B. Dacheux JL, Garnier DH. Terqui M, Colas G and Courot M (1979) Biochemical and physiological studies of androgen-binding protein in the reproductive tract of the ram. J Reprod Fert 57: 311–318
Jones R (1974) Absorption and secretion in the cauda epididymidis of the rabbit and the effects of degenerating spermatozoa on epididymal plasma after castration. J Endocr 63: 157–165
Jones R (1977) Effects of testosterone, testosterone metabolites and anti-androgens on the function of the male accessory glands in the rabbit and rat. J Endocr 74: 75–78
Jones R (1978) Comparative biochemistry of mammalian epididymal plasma. Comp Biochem Biophys 61B: 365–370
Jones R and Brown CR (1982) Association of epididymal secretory proteins showing oc-lactalbumin-like activity with the plasma membrane of rat spermatozoa. Biochem J 206: 161–164
Jones R and Dott HM (1980) Changes in luminal plasma and disappearance of spermatozoa from the ligated Cauda epididymidis of the androgen-deficient rabbit. J Reprod Fert 60: 65–72
Jones R and Glover TD (1973) The effects of castration on the composition of rabbit epididymal plasma. J Reprod Pert 34: 405–411
Jones R. von Glos KI and Brown CR (1980a) The synthesis of a sperm-coating protein in the initial segment of the rat epididymis is stimulated by factors in testicular fluid. IRCS Med Sci 8: 56
Jones R, Brown CR. von Glos KI and Parker MG (1980b) Hormonal regulation of proteins synthesis in the rat epididymis. Characterization of androgen-dependent and testicular fluid-dependent proteins Biochem J 188: 667–676
Jones R. von Glos KI and Brown CR (1981) Characterization of hormonally regulated secretory proteins from the caput epididymidis of the rabbit. Biochem J 196: 105–114
Jones R. Fournicer-Delpech S and Willadson SA (1982) Identification of androgen-dependent proteins synthesised in vitro by the ram epididymis. Reprod Nutr Develop 22: 495–504
Jones R, Riding PR and Parker MG (1983b) Effects of prolactin on testosterone-induced growth and protein synthesis in rat accessory glands. J Endocr 96: 407–416
Jones R. von Glos KI and Brown CR (1983c) Changes in the protein composition of rat spermatozoa during maturation in the epididymis. J Reprod Pert 67: 299–308
Kanka J and Kopeeny V (1977) An autoradiographic study of macromolecular synthesis in the epitheliumn of the ductus epididymidis in the mouse. I. DNA. RNA and Protein. Biol Reprod 16: 421–427
Kierszenbaum AL. Lea O. Petrusz P, French FS and Tres LL (1981) Isolation, culture, and immunocytoChemical characterization of epididymal epithelial cells from pubertal and adult rats. Proc Natn Acad Sci US 78: 1675–1679
Killian GJ and Chapman DA (1980) Glycerylphosphorylcholine, sialic acid and protein in epithelial cells isolated from the rat caput epididymidis by elutriation. Biol Reprod 22: 846–850
Kissinger C. Skinner MK and Griswold MDN (1982) Analysis of Sertoli cell-secreted proteins by two dimensional gel electrophoresis. Biol Reprod 27: 233–240
Klinefelter GR and Hamilton DW (1984) Organ culture of rat caput epididymal tubules in a perifusion chamber. J Androl 5: 243–258
Klinefelter GR and Hamilton DW (1985) Synthesis and secretion of proteins by perifused caput epididymal tubules, and association of secreted proteins with spermatozoa. Biol Reprod 33: 1017–1027
Klinefelter GR. Amann RP and Hammerstedt RH (1982) Culture of principal cells from the rat caput epididymidis. Biol Reprod 26: 885–901
Kochakian CD (1975) Free amino acids of sex organs of the mouse: regulation by androgen. Am J Physiol 228: 1231–1235
Kochakian CD (1976) Influence of testosterone on the concentration of hypotaurineand taurine in the reproductive tract of the male guinea pig, rat. and mouse. In: Taurine. Eds R Huxtable and A Barbeau, Raven Press. New York, pp 327–334
Kohane AC. Garberi JC. Cameo MS and Blaquier JA (1979) Quantitative determination of specific proteins in rat epididymis. J Steroid Biochem 11: 671–674
Kohane AC. Pineiro L and Blaquier JA (1983) Androgen-controlled synthesis of specific proteins in the rat epididymis. Endocrinology 112: 1590–1596
Kopečny V (1971) Epididymal luminal concents labelling after l4C- or 3H-lysine administration in the mouse. Acta Histochem 40: 116–122
Kopečny V (1976) Labelling of acrosomes in mouse spermatozoa by L-(1-3H)fucose. J Reprod Fert 47: 403–405
Kopečny V and Pech V (1977) An autoradiographic study of macromolecular synthesis in the epithelium of the ductus epididymidis in the mouse. II. Incorporation of L-fucose-1-3H. Histochem 50: 229–238
Kopečny V. Flechon JE and Pivko J (1984) Binding of secreted glycoproteins to spermatozoa in the mammalian epididymis: a fine-structure autoradiographic study. Anat Rec 208: 197–206
Koskimies AI and Kormano M (1975) Protein in fluids from different segments of the rat epididymis. J Reprod Pert 43: 345–348
Laporte P (1970) Acides sialiques epididymaires et maturite sexualle chez Ic rat. CR Soc de Biol 164: 2079–2984
Larsson LI, Fahrenkrug J, Schaffalitzky de Muckadell OB (1977) Occurrence of nerves containing vasoactive intestinal polypeptide immunoreactivity in the male genital tract. Life Sci 21: 503–508
Lavon U, Volcani R and Danon D (1971) The proteins of bovine spermatozoa from the caput and cauda epididymidis. J Reprod Fert 24: 219–232
Letts PJ, Meistrich ML, Bruce WR and Schaeter II (1974) Glycoprotein glycosyl-transferase levels during spermatogenesis in mice. Biochem Biophys Acta 343: 192–207
Lea OA. Petrusz P and French FS (1978) Purification and localization of acidic epididymal glycoprotein (AEG): a sperm coating protein secreted by the rat epididymis. Int J Androl Suppl 2: 592–607
Lea OA and French FS (1981) Characterization of an acidic glycoprotein secreted by principal cells of the rat epididymis. Biochem Biophys Acta 668: 370–376
Li TK (1975) The glutathione and thiol content of mammalian spermatozoa and seminal plasma. Biol Reprod 12: 641–646
Lojda Z (1975) The use of hexazonium-p-rosanilin in the histochemical demonstration of peptidases. Histochem 44: 323–335
Low MG and Kincade PW (1985) Phosphatidylinositol is the membrane-anchoring domain of the Thy 1-glycoprotein. Nature (Lond) 318: 62–64
Majumder GC (1981) Histone-mediated agglutination of epididymal spermatozoa and the occurence of histone receptors on the rat sperm surface. J Reprod Fert 62: 459–466
Matsuzawa T (1981) Substrate specificity and Histochemical distribution of aminopeptidase in rat testis and epididymis. Endocr Japan 28: 469–475
Mayorga L and Bertini F (1983) Two populations of hydrolase-containing particles in rat epididymis. J Cell Physiol 117: 135–139
Meister A (1973) On the enzymology of amino acid transport. Transport in kidney and probably other tissues is mediated by a cycle of enzyme reactions involving glutathione. Science 180: 33–39
Meister A (1974) Glutathione, metabolism and function via the γ-glutamylcycle. Life Sci 15: 177–190
Meister A and Tate SS (1972) Glutathione and related γ-glutamylcompounds: biosynthesis and utilization. Ann Rev Biochem 45: 559–604
Mercado Pichardo F., Villalohos Ramirez M, Dominguez Aguilar R and Rosado Garcia A (1982) Modifications in the apolar regions of the membrane of rabbit sperm cells during epididymal maturation. Archos Invest Med 13: 173–180
Mongkolsirikicat S and C’hulavatnatol M (1984) Phosphorylated secretory proteins from rat epididymis and their androgenic control. J Reprod Fert 72: 423–428
Moore HDM (1980) Localization of specific glycoproteins secreted by the rabbit and hamster epididymis. Biol Reprod 22: 705–718
Moore HDM (1981a) Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster. J Reprod Fert 61: 347–354
Moore HDM (1981b) Glycoprotein secretions of the epididymis in the rabbit and hamster: localization on epididymal spermatozoa and the effect of specific antibodies on fertilization in vivo. J exp Zool 215: 77–85
Moore HDM and Bedford JM (1979) Short-term effects of androgen withdrawal on the structure of different epithelial cells in the rat epididymis. Anat Rec 193: 293–312
Moore HDM and Pryor JP (1981) The comparative ultrastructure of the epididymis in monkeys and man: a search for a suitable animal model for studying epididymal physiology in Primates. Am J Primatol 1: 241–250
Moore HDM, Hartman TD. Brown AC. Smith CA and Ellis DH (1985) Expression of sperm antigens during spermatogenesis and maturation detected with monoclonal antibodies. Expl clin Immunogenet 2: 84–96
Morton Ü and Chang TSK (1973) The effect of fluid from the cauda epididymidis, scrum components and caffeine upon the survival of diluted epididvmal hamster spermatozoa. J Reprod Fert 35: 255–263
Morton BE. Fraser CF and Albagli L (1979a) Studies on factors in hamster caudal epididymal plasma and other sources which inhibit sperm dilution damage. Fert Steril 32: 99 106
Morton BE, Fraser CF and Sagadraca R (1979b) Inhibition of sperm dilution damage by purified factors from hamster caudal epididymal plasma and by defined diluents. Fert Steril 32: 107–114
Musto N, Gunsalas G, Cheng CY, Tsong SD, Goldstein M. Phillips D and Bardin (1983) Identification of androgen-binding proteins and their localization in the testes and male reproductive tract. In: Recent Advances in Male Reproduction. Molecular Basis and Clinical Implications. Eds R D’Agato. MB Lipsett. P Polosa and HJ van der Molen, Raven Press. New York, pp 37–45
Neutra M and Leblond CP (1966) Radioautographic comparison of the uptake of galactose-H3 and glucose–H3 in the Golgi region of various cells secreting glycoproteins or mucopolysaccharides. J Cell Biol 30: 137–150
Neumark H and Schindler 11(1967) Amino acids, amines and peptides of ram epididymal semen. J Reprod Fert 14: 469–471
Nicander L and Malmqvist M (1977) Ultrastructural observations suggesting merocrine secretion in the initial segment of the mammalian epididymis. Cell Tiss Res 184: 487–490
Niemi M and Tuohimaa P (1971) The mitogenic activity of testosterone in the accessory glands of the rat in relation to its conversion to dihydrotestosterone. In: Basic Action of Sex Steroid on Target Organs. Eds PO Hubinot. F Leroy and P Galand, Karger, Basel, pp 258–264
Oliphant G (1976) Removal of sperm-bound seminal plasma components as a prerequisite to induction of the rabbit acrosome reaction. Fert Steril 27: 28–38
Oliphant G and Brackelt BG (1973) Capacitation of mouse spermatozoa in media with elevated ionic strength and reversible decapacitation with epididymal extracts. Fert Steril 24: 948–955
Olson GF. and Hinton BT (1985) Regional differences in luminal fluid polypeptides of the rat testis and epididymis revealed by two-dimensional gel electrophoresis. J Androl 6: 20–34
Olson GE and Orgebin-Crist MC (1982) Sperm surface changes during epididymal maturation. Ann NY Acad Sci 383: 372–391
Olson GE. Jonas-Davies J. Hoffman LH and Orgebin-Crist MC (1983) Structural features of cultured epithelial cells from the adult rat epididymis. J Androl 4: 347–360
Ong DE. Crow J A and Chytil F (1982) RadioimmunoChemical determination of cellular retinol- and cellular retinoic acid-binding proteins in cytosols of rat tissues. J Biol Chem 257: 13385–13389
Orgebin-Crist MC and Jiane J I (1979) Properties of prolactin receptor from the rabbit epididymis. Biol Reprod 21: 135–139
Orgebin-Crist MC and Menezo Y (1980) A continuous flow method for organ cultrue of rabbit epididymis: morphology, amino acid utilization, glucose uptake. RNA. and protein synthesis. J Androl 1: 289–298
Papkoff H (1966) Glycoproteins with biological activity. In: Glycoproteins and their composition. structure, and function. Section 9. Ed A Gottschalk. Elsevier Pub Co, Amsterdam, pp 532–557
Pekary AE, Ross R, Kernion JOE and Hershman JM (1983) Human reproductive tissues contain thyrotropin releasing hormone (TRH) and TRH-homologous peptides. J Androl 4: 408–414
Pekary AE. Yamada T. Sharp B, Bhasin S, Swerdloff RS and Hershman JM (1984) Somatostatin-14 and -28 in the male rat reproductive-system. Life Sci 34: 939–946
Pelliniemi I J. Dym M. Gunsalas GL, Musto NA. Bardin CW and Fawcett DW (1981) Immunocytochemical localization of androgen-binding protein in the male rat reproductive tract. Endocrinology 108: 925–931
Peyre A and Laporte P (1966) Action de la testosterone et de l’oestradiol sur les sialo-proteins de la queue dc I’epididyme de rat impubére castré. CR Soc de Biol 160: 2178–2180
Poirier GR and Jackson J (1981) Isolation and characterization of two proteinase inhibitors from the male reproductive tract of mice. Gamete Res 4: 555–569
Poirier CR and Nicholson N (1984) Distribution of a proteinase inhibitor of epididymal origin in the tissues and secretions of the male reproductive tract of mice. J exp Zool 230: 465–472
Porter SB. Ong DE, Chytil F and Orgebin-Crist MC (1985) Localization of cellular retinol- binding protein and cellular retinoic acid binding protein in the rat testis and epididymis. J Androl 6: 197–212
Purvis K and Hansson V (1978) Androgens and androgen-bmding protein in the rat epididymis. J Reprod Fert 52: 59–63
Rajalakshmi M (1985) Hormonal-regulation of specific proteins in the rat epididymis. Archs Androl 14: 181–185
Rajalakshmi M and Prasad MRN (1968) Changes in the sialic acid content of the accessory glands of the rat. J Endocr 41: 471–476
Rajalakshmi M and Prasad MRN (1969) Changes in sialic acid in the testis and epididymis of the rat during the onset of puberty. J Endocr 44: 379–385
Reyes A. Parra A. Chavarria ME, Goicoechea B and Rosado A (1979) Effect of prolactin on the calcium binding and/or transport of ejaculated and epididymal human spermatozoa. Fert Steril 31: 669–672
Riar SS. Setty BS and Kar AB (1973) Studies on the physiology and biochemistry of mammalian epididymis. Biochemical composition of epididymis. A comparative study. Fert Steril 24: 355–363
Robaire B and Hales BF (1982) Regulation of epididymal glutathione S-transferases: effects of orchiectomy and androgen replacement. Biol Reprod 26: 559–565
Rohr H, Oberholzer M, Bartsch G and Keller M (1976) Morphometry in experimental pathology: methods, baseline data, and applications. In: International review of Experimental Pathology. Volume 15. Eds GW Richter and MA Epstein, Acad Press, London pp 233–325
Roy N, Majumder GC and Chakrabarty CK (1985) Occurrence of specific glycoprotein factors in goat epididymal plasma that prevent adhesion of spermatozoa to glass. Andrologia 17: 200–206
Rufo GA, Singh JP, Babcock DF and Lardy HA (1982) Purification and characterization of a calcium transport inhibitor protein from bovine seminal plasma. J Biol Chem 257: 4627–4632
Rufo GA. Schoff PK and Lardy HA (1984) Regulation of calcium content in bovine spermatozoa. J Biol Chem 259: 2547–2552
Russell LD. Peterson RN, Hunt W and Strack LE (1984) Posttesticular surface modifications and contributions of reproductive tract fluids to the surface polypeptide composition of boar spermatozoa. Biol Reprod 30: 959–978
Rutenburg AM, Kim H, Fischbein JW, Hanker JS, Wasserkrug IIL and Seligman AM (1969) Hislochemical and ultrastructural demonstration of γ-glutamyl transpeptidase activity. J Histo Cyto 17: 517–526
Schachter H (1978) Glycoprotein synthesis. In: The glycoconjugates. Volume II. Eds MI Horowitz and W Pigman. Acad Press. New York, pp 87–181
Sedláková E. Dostál J and Malousek J (1968) Serum albumin in pig epididymis. Comp Biochem Physiol 26: 143–148
Scike H, Yokoyama M, Iwata II, Ochi K. Takeuchio M. Takada Y, Hiwada K and Kokubu T (1985) Production of angiotensin-converting enzyme in rat epididymis. Andrologia 17: 406–410
Setchell BP. Hinks NT. Voglmayr JK and Scott TW (1967) Amino acids in ram testicular fluid and semen and their metabolism by spermatozoa. Biochem J 105: 1061–1066
Setty BS, Riar SS and Kar AB (1979) Androgen control of epididymal function in rhesus monkey and rabbit. Fert Steril 28: 674–681
Sexton TJ. Amann RP and Flipse RJ (1971) Free amino acids and protein in rete testis fluid, vas deferens plasma, and accessory sex gland fluid and seminal plasma of the conscious bull. J Dairy Sci 54: 412–416
Sexton TJ, Amann RP and Flipse RJ (1972) Oxidative metabolism of glutamate by testicular, epididymal, and ejaculated bovine spermatozoa in the presence of rete testis fluid and seminal plasma. J Dairy Sci 55: 1005–1009
Sharp B. Pekary AE. Meyer NV and Hersham JM (1980) endorphin in male rat reproductive organs. Biochem Biophys Res Common 95: 618–623
Shimazaki J. Yamanaka II. Taguchi I and Shida K (1976) Free amino acids in the caput and the cauda epididymis of adult rats. Endocr Japan 23: 149–156
Sheth AR. Gunjikar AN and Shah GV (1981) The presence of progressive motility sustaining factor (PMSF) in human epididymis. Andrologia 13: 142–146
Silvestroni L. Satori C, Modesti A and Frajese G (1982) Harmless technique for removal of the cell coat from human spermatozoa. Experientia 38: 672–673
Silvestroni L. Sartori C. Modesti A and Frejese G (1983) Advances in molecular andrology: biochemical and ultrastructural studies on human sperm plasma membrane. In: Recent Advances in Male Reproduction. Molecular Basis and Clinical Implications. Eds R D’Agat, MB Lipsett, P Polosa and HJ Van Der Molen. Raven Press, New York, pp 1–11
Sirett DAN, Rolin Jacqemyns CF, Quivy JI and Rousseau GG (1984) Fffccts of dicyclohexane compounds in vivo on rat epididymal spermatozoa. Proc of 23rd Harden Conference. “Molecular and Cellular Aspects of Reproduction”, Wye Colege, University of London, Sept 1984 (Abst)
Skinner MK and Griswold MD (1980) Sertoli cells synthesize and secrete transferrin-like protein. J Biol Chem 255: 9523–9525
Skinner MK and Griswold MD (1982) Secretion of testicular transferrin by cultured Sertoli cells is regulated by hormones and retinoids. Biol Reprod 27: 211–221
Skinner MK and Griswold MD (1983) Sertoli cells synthesize and secrete a ceruloplasmin-like protein. Biol Reprod 28: 1225–1229
Stanek R and Dostäl J (1974) Proteins in bull rete testis and cauda epididymis plasma. Comp Biochem Biophys 48B: 613–618
Strittmatter SM and Snyder SH (1984) Angiotensin-Converting enzyme in the mnale rat reproductive system: autoradiographic visualization with (3H)captopril. Endocrinology 115: 2332–2341
Suzuki F (1981) Changes in intramembranous particle distribution in epididymal spermatozoa of the boar. Anat Ree 199: 361–376
Suzuki F and Nagano T (1980) Epididymal maturation of rat spermatozoa studied by thin sectioning and freeze fracture. Biol Reprod 22: 1219–1231
Sweet C and Zull JF. (1969) Activation of glucose diffusion from egg lecithin liquid crystals by serum albumin. Biochem Biophys Acta 173: 94–103
Sylvester SR. Skinner MK and Griswold MD (1984) A sulphated glycoprotein synthesized by Sertoli cells and by epididymal cells is a component of the sperm membrane. Biol Reprod 31: 1087–1801
Tadolini B. Wilson J. Reddy PRK and Williams-Ashman HG (1977) Characteristics and hormonal control of some glycoprotein glycosyltransferase reactions in male reproductive organs. Advances in Enzyme Regulation, Ed G Weber, Volume 15. pp 319–336
Tarter TH and Alexander NJ (1984) Complement-inhibiting activity of seminal plasma. Am J Reprod Immunol 6: 28–32
Tezön JG and Blaqier JA (1981) The organ culture of human epididymal tubules and their response to androgens. Mol Cell Endocr 21: 233–242
Tezön JG. Cuasnicü PS, Scorticati C and Blaquier JA (1982a) Development and characterization of a model system for the study of epididymal function in man. In: Physiopathology of Hypophysial Disturbances and Diseases of Reproduction. Eds A DENicola. JA BLaquiEr and RJ Soto. Alan Liss Inc. New York, pp 251 –275
Tezön JG, Vazquez M, Pineiro L, De Larminat MA and Blaquier J (1982b) The effect of androgens in the human epididymis in organ culture. In: Therapy in Andrology. Pharmacological, Surgical and Pathological Aspects. Eds GF Mechini-Fabris, W Pasini and L Martini. Elsevier. New York pp. 53–60
Tezdn JG. Vazquez MH, Pineiro L. deLarminat MA and Blaquier JG (1985a) Identification of androgen-induoed proteins in human epididymis. Biol Reprod 32: 584–590
Tezön JG. Ramella E. Cameo MS. Vazquez MH and Blaquier, JG (1985b) Immunocytochemical localization of secretion antigens in the human epididymis and their association with spermatozoa. Biol Reprod 32: 591–597
Thomas TS. Reynolds AL and Oliphant G (1984) Evaluation of the site of synthesis of rabbit sperm acrosomc stabilizing factor using immunocytochemical and metabolic labeling techniques. Biol Reprod 30: 693–705
Tkacz JS and Lampen JO (1975) Tunicamycin inhibition of polyisoprenol N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes. Biochem Biophys Res Commun 65: 248–257
Toowicharanont P and Chulavatnatol M (1983a) Measurement of anionic sites of rat epididymal spermatozoa using tritiated polycationized ferritin. J Reprod Fert 69: 303–306
Trcmblay RR, Chapdelainc P and Mailhot J (1979) α-l,4-glucosidase activity in human semen: variations with number and motility of spermatozoa. Fert Steril 31: 592–593
Tremblay RR. Chapdelaine P and Dube JY (1982) Neutral α-1,4-glucosidase in human seminal plasma: molecular forms in varicoccle abd after vasectomy. Fert Steril 38: 344–348
Tsang AYF. Lee WM and Wong PYD (1981) Effects of antifertility drugs on epididymal protein secretion, aquisition of sperm surface proteins and fertility in male rats. Int J Androl 4: 703–712
Tsong SI), Phillips DM, Halmi N, Krieger D and Bardin CW (1982b) β-endorphin is present in the male reproductive tract of five species. Biol Reprod 27: 755–764
Tung PS and Fritz IB (1985) Immunolocalization of clusterin in the ram testis, rele testis, and excurrent ducts. Biol Reprod 33: 177–186
Turner TT (1979) On the epididymis and its function. Invest Urol 16: 311–321
Turner TT and Giles RD (1982) Sperm motility-inhibiting factror in rat epididymis. Am J Physiol 242: R199–R203
Turner TT and Giles RD (1982b) The effects of cyclic adenine nucleotides, phosphodiesterase inhibitors, and cauda epididymal fluid on the motility of rat epididymal spermatozoa. J Androl 3: 134–139
Turner TT and Howards SS (1978) Factors involved in the initiation of sperm motility. Biol Reprod 18: 571–578
Turner TT and Reich GW (1985) Cauda epididymal sperm motility: a comparison among 5 species. Biol Reprod 32: 120–128
Turner TT, Plesums JL and Cabot CL (1979) Luminal fluid proteins of the male rat reproductive tract. Biol Reprod 21: 883 - 890
Usselman MC and Cone RA (1983) Rat sperm are mechanically immobilized in the caudal epididymis by “immobilin” a high molecular weight glycoprotein. Biol Reprod 29: 1241–1253
Usselman MC, Cone RA and Rossignol P (1985) Rat cauda epididymal fluid is a mucus. J Androl 6: 315–320
Vanha-Perttula T, Mather JP, Bardin CW, Moss SB and Bellve AR (1985) Localization of the Angiotensin converting enzyme-activity in testis and epididymis. Biol Reprod 33: 870–877
Velazquez A. Rosado A and Rosado A (1975) Participation of vitamin A in the maturation of rabbit spermatozoa. Int J Fert 20: 151–155
Vendrely C and Durliat M (1968) Etude autoradiographique de Tinflucnce de Thormone male sur l’incorporation de leucine dans le tractus genital de la souris blanche. Archive d’Anat Histol Embryol 51: 735–740
Vernon RB. Mueller CH, Herr JC, Feuchter FA and Eddy EM (1982) Epididymal secretion of a mouse sperm surface component recognized by a monoclonal antibody. Biol Reprod 26: 523–535
Vreeburg JTM (1975) Distribution of testosterone and 5α-dihydrotcstostcrone in rat epididymis and their concentrations in efferent duct fluid. J Endocr 67: 203–210
Voglmayr JK, Musto NA, Saksena SK. Brown-Woodman PDC. Marley PB and White IG (1977) Characteristics of semen collected from the cauda epididymidis of conscious rams. J Reprod Pert 49: 245–251
Voglmayr JK, Fairbanks G, Jackowitz MA and Colella JR (1980) Post-testicular developmental changes in the ram sperm cell surface and their relationship to luminal fluid proteins of the reproductive tract. Biol Reprod 22: 655–667
Voglmayr JK, Fairbank G. Vespa DB and Colella JR (1982) Studies on the mechanisms of surface modifications in ram spermatozoa during the final stages of differentiation. Biol Reprod 26: 483–500
Wenstrom JC and Hamilton DW (1980) Dolichol concentration and biosynthesis in rat testis and epididymis. Biol Reprod 23: 1054 - 1059
Wenstrom JC and Hamilton DW (1984) Synthesis and secretion of proteins in vitro by isolated epithelial strips from the proximal and distal vas deferens of the rat. Int J Androl 7: 215–235
White MG, Huang YS, Tres LL and Kierszenbaum AL (1982) Structural and functional aspects of cultured epididymal epithelial cells isolated from pubertal rats. J Reprod Pert 66: 475–484
Wilson RM and Griswold MD (1979) Secreted proteins from rat Sertoli cells. Exp Cell Res 123: 127–13
Witorsch RJ (1978) ImmunoHistochemical studies of prolactin binding in sex acccssory organs of the male rat. J Histo Cyto 26: 565–580
Wong PYD and Tsang AYF (1982) Studies on the binding of a 32K rat epididymal protein to rat epididymal spermatoza. Biol Reprod 27: 1239–1246
Wong PYD and Tsang AYF (1983) Radioimmunoreactivity and receptor binding activity of specific epididymal proteins of the rat and other species. Biochem Biophys Res Commun 114: 653–656
Wong PYD, Au CL and Ngai HK (1978) Electrolyte and water transport in rat epididymis. Its possible role in sperm maturation. Int J Androl Suppl 5: 608–628
Wong PYD. Au CL and Ngai HK (1979) Some characteristics of salt and water transport in the rat epididymis. In: The Spermatozoon. Eds DW Faweett and JM Bedford, Urban and Schwarzenberg. Baltimore, pp 57–63
Wong PYD, Tsang AYF, Lee WM and Li CM (1980) Secretion of the rat cauda epididymidis. Archs Androl 5: 327–336
Wong PYD, Isang AYF and Lee WM (1981) Origin of the luminal fluid proteins of the rat epididymis. Int J Androl 4: 331–341
Wong PYD, I’sang AYF and Lee WM (1982a) Secretion of macromoleculaes by the rat epididymis. Int J Androl Suppl 5: 34–47
Wong PYD, Tsang AYF and Wang C (1982b) Absence of a sperm coating protein after epididymovasostomy. J Androl 3: 205–208
Wnght WW, Musto NA, Mather JT and Bardin CW (1981) Sertoli cells secrete both testis-specific and serum proteins. Proc Natn Acad Sci US 78: 7565–7569
Yeung CH and Cooper TG (1982) Ultrastructure of the perfused rat epididymis: effect of luminal sodium ion concentration. Cell Tiss Res 226: 407–425
Yoo BY, Ji I and Ji TH (1982) Changes in protein composition of bovine spermatozoa during epididymal maturation. Can J Zool 60: 2970–2972
Zchcb R and Orr GA (1984) Characterization of a maturation-associated glycoprotein on the plasma membrane of rat caudal epididymal sperm. J Biol Chem 259: 839–848
Zimmerman KJ. Crabo BG, Moore R, Wcisberg S, Diebel FC and Graham EF (1979) Movements of sodium and potassium into epididymal boar spermatozoa. Biol Reprod 21: 173–179
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Cooper, T.G. (1986). Epididymal Secretion and Resorption of Proteins. In: The Epididymis, Sperm Maturation and Fertilisation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71471-9_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-71471-9_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-71473-3
Online ISBN: 978-3-642-71471-9
eBook Packages: Springer Book Archive