Successful In Vitro Fertilization of Frozen-Thawed Rabbit and Human Oocytes

  • K. Diedrich
  • S. Al-Hasani
  • H. van der Ven
  • D. Krebs


The success rate of in vitro fertilization (IVF) and embryo transfer is still low and needs improvement [1]. Many steps of this difficult treatment could be improved for example ovarian stimulation [2, 3], oocyte retrieval [4, 5], sperm preparation [6, 7], IVF and embryo transfer techniques [8]. Bearing this in mind, the pregnancy rate still does not exceed 20%, although the embryo transfer rate in some clinics is more than 80%.


Embryo Transfer Zona Pellucida Human Oocyte Vitro Fertilization Unfertilized Oocyte 
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  1. 1.
    Seppälä M (to be published) The world collaboration report on in vitro fertilisation and embryo replacement. Current state of the art in January 1984. Ann NY Acad SciGoogle Scholar
  2. 2.
    Diedrich K, Al-Hasani S, van der Ven H, Lehmann F, Krebs D (1983) Ovarielle Stimulation in einem in vitro Fertilisationsprogramm. Geburtshilfe Frauenheilkd 43:486PubMedCrossRefGoogle Scholar
  3. 3.
    Jones GS (1983) The use of human menopausal gonadotropin for ovulation stimulation in patients for in vitro fertilization. Infertility 6:11Google Scholar
  4. 4.
    Lehmann F, Diedrich K, van der Ven H, Al-Hasani S, Hamerich U, Krebs D (1984) Die Durchführung der laparoskopischen Follikelpunktion und Eizellgewinnung zur In-vitro-Fertilisation. Geburtshilfe Frauenheilkd 44:388PubMedCrossRefGoogle Scholar
  5. 5.
    Wikland M, Nilsson L, Hansson R, Hamberger L, Janson PO (1983) Collection of human oocytes by the use of sonography. Fertil. Steril. 39:603–608PubMedGoogle Scholar
  6. 6.
    Trounson AO, Morh LR, Wood C, Leeton JF (1982) Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fertil 64:285PubMedCrossRefGoogle Scholar
  7. 7.
    Diedrich K, Krebs D (1983) Extracorporal Befruchtung und Embryotransfer in der gynäkologischen Praxis. In: Jüdes U (ed) In vitro-Fertilisation und Embryotransfer (Retortenbaby). Wissenschaftliche Verlagsgemeinschaft, StuttgartGoogle Scholar
  8. 8.
    Diedrich K (1983) Embryotransfer. In: Krebs D (ed) In vitro Fertilisation und Embryotransfer. Urban and Schwarzenberg, MünchenGoogle Scholar
  9. 9.
    Al-Hasani S, Trotnow S, Barthel M (1982) Kryokonservierung von Kaninchenembryonen des Acht-Zell-Stadiums im automatisierten “Offenen System”. Geburtshilfe Frauenheilkd 41:847–908Google Scholar
  10. 10.
    Downing BD, Mohr LR, Trounson AO, Feeman LE, Wood C (1985) Birth after transfer of cryopreserved embryos. Med J Aust 142:409–410PubMedGoogle Scholar
  11. 11.
    Al-Hasani S, Trotnow S, Köhnlein M (1983) Kryokonservierung von Mäuseembryonen nach dem Zwei-Stufen-Verfahren im automatisierten “Offenen System”Google Scholar
  12. 12.
    Trounson A, Mohr L (1983) Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 305:707–709PubMedCrossRefGoogle Scholar
  13. 13.
    Zeilmaker GH, Alborda AT, van Gient I, Rijkmans CMPM, Drogendijk AC (1984) Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 42:293–296PubMedGoogle Scholar
  14. 14.
    Siebenrübl E, Trotnow S, Kniewald T, Hünlich T (1986) Humanmedizinische Aspekte der Embryokonservierung. In: Schill, Bollmann (eds) Spermakonservierung, Insemination, In-vitro-Fertilisation. Urban and Schwarzenberg, München pp 230–237Google Scholar
  15. 15.
    Diedrich K, Wildt L, Diedrich C, van der Ven H, Al-Hasani S, Lehmann F, Krebs D (1985) Ovarielle Stimulation für die extrakorporale Befruchtung. Fertilität 1:26–31Google Scholar
  16. 16.
    Edwards RG, Steptoe PC (1983) Current state of in vitro fertilization and implantation of human embryos. Lancet III:1265CrossRefGoogle Scholar
  17. 17.
    Edwards RG (1981) Test tube babies 1981. Nature 293:253–256PubMedCrossRefGoogle Scholar
  18. 18.
    Odenbach PE (1986) Die berufsrechtlichen Regelungen für In-vitro-Fertilisation, Embryotransfer und für die Forschung an Embryonen. Deutsches Ärzteblatt 12:781–784Google Scholar
  19. 19.
    Al-Hasani S, Tolksdorf A, Diedrich K, van der Ven H, Krebs D (to be published) Successful in vitro fertilisation of frozen-thawed rabbit oocytes. Human ReprodGoogle Scholar
  20. 20.
    Leibo SP (1980) Water permeability and its activation energy of fertilized and unfertilized mouse ova. J Membr Biol 53:179–188PubMedCrossRefGoogle Scholar
  21. 21.
    Von Feichtinger W, Kafka I, Kogosowski A, Kemeter P (1986) Erste Ergebnisse eines Kryokonservierungsprogrammes menschlicher Eizellen und Embryonen. Zentralbl Gynäkol 108:305–315PubMedGoogle Scholar
  22. 22.
    Cohn S, Simons RF, Fehilly CB, Edwards RG, Hewitt J, Rowland GF, Steptoe PG, Webster M (1985) Letter: Birth after replacement of hatching blastocyst cryopreserved at expanded blastocyst stage. Lancet 1:647CrossRefGoogle Scholar
  23. 23.
    Schneider U (1986) Cryobiological principials of embryos freezing. J In Vitro Fertil Embryo Transfer (1)Google Scholar
  24. 24.
    Mohr L, Trounson A, Freeman L (1985) Deep freezing and transfer of human embryos. J Fertil Embryo Transfer 1:1CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • K. Diedrich
    • 1
  • S. Al-Hasani
    • 1
  • H. van der Ven
    • 1
  • D. Krebs
    • 1
  1. 1.University Women’s Clinic of BonnFederal Republic of Germany

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