Experimental Genetics of the Mammalian Embryo
In recent years, several approaches suitable for the analysis of gene activity during mammalian embryogenesis have been realized in the mouse and I should like to briefly summarize these new results and discuss their relevance and applicability to experimental embryology. First, enucleation by removing either both pronuclei from the fertilized egg or the female pronucleus from the unfertilized egg provides an experimental means of analyzing the protein pattern derived from maternal mRNA stored during oogenesis. Secondly, microsurgical removal of one of the two pronuclei from the fertilized mouse egg makes it possible to study independently the maternal or paternal genome for its protein synthesis capacity during development. Thirdly, in nuclear transplantations the egg genome is replaced by a somatic cell nucleus in order to reveal the developmental potential of nuclei originating from different cell types and lineages at various stages of differentiation. Fourthly, in gene transplantations cloned recombinant DNA molecules are introduced into the developing organism via injection into the pronucleus of the fertilized egg to examine their presence, expression and regulation during mouse development.
KeywordsElectrophoresis Polypeptide Polyacrylamide Dodecyl Thymidine
Unable to display preview. Download preview PDF.
- Chapman VM, West JD, Adler DA (1977) In: Sherman MI (ed) Concepts in mammalian embryogenesis. MIT, Cambridge, pp 94–135Google Scholar
- Illmensee K, Hoppe PC (1981b) In: Sauer HW (ed) Progress in developmental biology. Fischer, Stuttgart, pp 67–74Google Scholar
- Illmensee K, Bürki K, Hoppe PC, Ullrich A (1981) In: Brown DD, Fox CF (eds) Developmental biology using purified genes. ICN-UCLA symposia on molecular and cellular biology, vol 23. Academic, New York, pp 607–619Google Scholar
- Piko L, Clegg KB (1981) IX. Congress int. soc. dev. biol, Basel (abstracts) p 236Google Scholar
- Snell GD, Stevens LC (1968) In: Green EL (ed) Biology of the laboratory mouse. Dover, New York pp 205–245Google Scholar
- Stevens LC (1975) In: Markert CL, Papaconstantinou J (eds) The developmental biology of reproduction. 33rd symp soc develop Biol. Academic, New York, pp 93–106Google Scholar