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Glial-Neuronal Communication in Development and Regeneration pp 3–25Cite as

From Neuroepithelium to Mature Astrocytes

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Part of the book series: NATO ASI Series ((ASIH,volume 2))

Abstract

In this chapter I will describe the differentiation of astrocytes of mouse neopallium in culture based mainly on our own observations. Cells were obtained from mouse embryos of different developmental stages, from the neural tube of early embryos to the brain of paranatal animals. The neopallium was dissected, then the cells were disaggregated and planted in culture in dilutions adjusted so as to be conducive to the formation of cell colonies rather than a monolayer of cells (22, 28). Such cultures select cells that (a) adhere to the substratum (in this case, the plastic of the tissue culture petri dishes, and (b) proliferate to form colonies. Approximately 25 per cent of colonies in our cultures start from single cells, thus forming true clones, others form from two or a few cells. Spatial relationships and interactions between cells in the colony determine its gross morphology and this is so constant that it identifies the various types of colony and, in turn, the cells composing them (23, 26).

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N OWO

    S. Fedoroff

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  1. S. Fedoroff
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  1. Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Str. 3, 3400, Göttingen, Germany

    Hans H. Althaus

  2. Max-Planck-Institut für biophysikalische Chemie, Am Faßberg, 3400, Göttingen-Nikolausberg, Germany

    Wilfried Seifert

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© 1987 Springer-Verlag Berlin Heidelberg

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Fedoroff, S. (1987). From Neuroepithelium to Mature Astrocytes. In: Althaus, H.H., Seifert, W. (eds) Glial-Neuronal Communication in Development and Regeneration. NATO ASI Series, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71381-1_1

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  • DOI: https://doi.org/10.1007/978-3-642-71381-1_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71383-5

  • Online ISBN: 978-3-642-71381-1

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