Study on Membrane Fluidity of Liver Peroxisomes

  • H. Hayashi
  • K. Nakata
  • F. Hashimoto
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The membrane fluidity of liver peroxisomes from normal and clofibrate-treated rats was investigated by using a fluorescence probe, 1-anilinonaphthalene-8-sulfonate (ANS). The excitation maximum of the probe was changed from 347 nm to 380 nm and the emission maximum from 493 nm to 463 nm in the presence of peroxisomes, and fluorescence intensity was enhanced more than 50 times. These results suggest that ANS was bound to the peroxisomal membrane. Fluorescence depolarization (P-value) was determined with such ANS- labeled peroxisomes. The P-values of peroxisomes from both normal and clofibrate-treated gradually decreased with increasing temperature, but that of clofibrate-treated peroxisomes was always smaller than that of normal peroxisomes. P-values were observed at 37° C for 60 min. The average P-value of normal peroxisomes was 0.270, while that of clofibrate-treated peroxisomes was 0.248. These results indicate that the membrane fluidity of liver peroxisomes is increased by the treatment with clofibrate. treatment of rats with another inducer of liver peroxisome proliferaton, di (2- ethylhexyl)phthalate, had an effect similar to that of clofibrate. However, alloxan treated (diabetic) rats showed no change of peroxisomal membrane fluidity.

Keywords

Cholesterol Porosity Peroxi Sucrose Mercury 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    De Duve, C.f and Baudhuin, P., (1966) Physiol. Rev., 46, 323–357.PubMedGoogle Scholar
  2. 2.
    Baudhuin, P.,Beaufay, H., and De Duve, C., (1965) J. Cell Biol. 26, 219 243.PubMedCrossRefGoogle Scholar
  3. 3.
    Reddy, J.K., Chiga, A., and Svoboda, D., (1971) Biochem. Biophys. Res. Commun 43, 318–324.PubMedCrossRefGoogle Scholar
  4. 4.
    Hayashi, H., Suga, T., and Niinobe, S., (1975) J. Biochem. 77, 1199–1204.PubMedGoogle Scholar
  5. 5.
    Novikoff, A.B., Novikoff, P.M., Davis, C., and Quintana, N., (1973) J. Histochem. Cytochem. 21, 737–755.PubMedCrossRefGoogle Scholar
  6. 6.
    Svoboda, D., Grady, H., and Azarnoff, D., (1967) J. Cell Biol. 35, 127–151.PubMedCrossRefGoogle Scholar
  7. 7.
    Jones, G.L., and Masters, C.J., (1978) Arch. Biochem. Biophys. 187, 431–440.PubMedCrossRefGoogle Scholar
  8. 8.
    Miura, S., Mori, M., Takiguchi, M., Tatibana, M., Furuta, S., Miyazawa, S., and Hashimoto, T., (1984) J. Biol. Chem. 259, 6397–6402.PubMedGoogle Scholar
  9. 9.
    Lazarow, P.B .. (1978) J. Biol. Chem. 253, 1522–1528.PubMedGoogle Scholar
  10. 10.
    Leighton, F., Poole, B. Beaufay, H., Baudhin, P., Coffey, J.W., Fowler, S.„ and De Duve, C., (1968) J. Cell Biol. 37, 482–513.PubMedCrossRefGoogle Scholar
  11. 11.
    Hayashi, H., Suga, T., and Niinobe, S., (1971) Biochim. Biophys. Acta 252, 58–68.PubMedCrossRefGoogle Scholar
  12. 12.
    Hayashi, H., Hino, S., Yamasaki, F., Watanabe, T., and Suga, T., (1981) Biochem. Pharmacol. 30, 1817–1822.PubMedCrossRefGoogle Scholar
  13. 13.
    Havashi, H., and Suga, T., (1978) J. Biochem. 84, 513–520.Google Scholar
  14. 14.
    Bradford, M.M., (1976) Anal. Biochem. 72, 248–254.PubMedCrossRefGoogle Scholar
  15. 15.
    Lowry, O.H., Rosebrough, N.T., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
  16. 16.
    Inbar, H., Inbar, M., (1974) J. Mol. Biol. 85, 603–615.PubMedCrossRefGoogle Scholar
  17. 17.
    Fuchs, P., Parola, A., Robbins, P.W., and Blout, E.R., (1985) Proc. Natl. Acad. Sci. 72, 3351–3354.CrossRefGoogle Scholar
  18. 18.
    Ardrich, M.P., and Vanderkooi, J.M., (1976) Biochemistry 15, 1257–1261.CrossRefGoogle Scholar
  19. 19.
    Osurai, T., and Hashimoto, T., (1979) J. Biochem. 85, 131–139.Google Scholar
  20. 20.
    Horie, S., Ishii, H., and Suga, T. (1981) J. Biochem. 90, 1691–1696.PubMedGoogle Scholar
  21. 21.
    Crane, D.I. and Masters, C.J. (1986) Biochim. Biophys. Acta 876, 256–263.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Hayashi
    • 1
  • K. Nakata
    • 1
  • F. Hashimoto
    • 1
  1. 1.Department of Physiological ChemistryJosai UniversitySakado, SaitamaJapan

Personalised recommendations