The Context of T Cell Receptor β Chain Genes Among Wild and Inbred Mouse Species

  • K. Huppi
  • L. A. D’Hoostelaere
  • E. Jouvin-Marche
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 127)


The organization of the mouse T cell receptor (TCR) genes has been extensively reviewed (Hood 1985). The structure of the TCR alpha (α), beta (β) and gamma (γ) genes is analogous to the immunoglobulin (Ig) gene organization in the mouse. This homology probably reflects similar requirements for diversity in binding antigen. The major source of diversity for Ig genes comes from (1) multiple germline variable (VK, Vλ and VH) regions, diversity (DH) regions and joining (Jj(, JX and Jh) elements; (2) combinatorial joining of V, D and J; (3) somatic mutation and (4) multiple alleles present in inbred and wild mice (Tonegawa 1983). Preliminary findings among the TCRα and β chains of the mouse inbred strains indicate fewer germline variable (VTα, VTβ) genes are available (30–40 each) (Becker 1985; Barth 1985; Behlke 1985a) than VK and VH genes (100–300 each) (Valbuena 1978; Cory 1981; Brodeur and Riblet 1984). Furthermore, studies indicate that the frequency of somatic mutation in TCRα and TCRβ chain genes although present is as much as 10% or less than that of Ig genes (Augustin and Sim 1984; Ikuta 1985).


Mouse Inbred Strain Wild Mouse Mouse Species Restriction Fragment Pattern Immunoglobulin Heavy Chain Variable Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arden B, Klotz JL, Siu G, Hood L (1985) Diversity and structure of genes of the α family of mouse T-cell antigen receptor. Nature 316: 783–787PubMedCrossRefGoogle Scholar
  2. Augustin A, Sim GK (1984) T-cell receptors generated via mutations are specific for various major histocompatibility antigens. Cell 39:5–12PubMedCrossRefGoogle Scholar
  3. Barth RK, Kim BS, Lon NC, Hunkapiller T, Sobieck N, Winoto A, Gershenfeld H, Hansburg D, Weissman I, Hood L (1985) The murine T-cell receptor uses a limited repertoire of expressed Vβ gene segments. Nature 316:517–522PubMedCrossRefGoogle Scholar
  4. Becker DM, Patten P, Chien Y-h, Yokota T, Eshhar Z, Giedlin M, Gascoigne NR, Goodnow L, Wolf R, Arai K, Davis MM (1985) Variability and repertoire size of T-cell receptor Va gene segments. Nature 317:430–434PubMedCrossRefGoogle Scholar
  5. Behlke MA, Spinella DE, Chou HS, Sha W, Hartl DL, Loh DY (1985) T-cell receptor β-chain expression: dependence on relatively few variable region genes. Science 229:566–570PubMedCrossRefGoogle Scholar
  6. Behlke, MA, Chou HS, Huppi K, Loh DY, Murine T-cell receptor V β deletion mutants. Proc Natl Acad Sci (USA) in pressGoogle Scholar
  7. Brodeur PH, Riblet R (1984) The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes. Eur J Immunol 14: 922–926.PubMedCrossRefGoogle Scholar
  8. Cazenave PA, Bonhomme F, Guenet J-L, Kindt TJ (1985) Correlation of CTβ phenotype with origins of laboratory mouse strains (this edition)Google Scholar
  9. Cory S, Tyler BM, Adams JM (1981) Sets of immunoglobulin V genes homologous to ten cloned V sequences: implications for the numbers of germline V genes. J Mol Appl Genet 1:103–116PubMedGoogle Scholar
  10. D’Hoostelaere LA, Jouvin-Marche E, Huppi K (1985) Localization of CTβ and CK on mouse chromosome 6. Immunogenetics 22:277–283PubMedCrossRefGoogle Scholar
  11. Epstein R, Roehm N, Marrack P, Kappler J, Davis MM, Hedrick S, Cohn M (1985) Genetic markers of the antigen-specific T cell receptor locus. J Exp Med 161:1219–1224PubMedCrossRefGoogle Scholar
  12. Hedrick SM, Nielsen EA, Kavaler J, Cohen DI, Davis MM (1984) Sequence relationships between putative T-cell receptor polypeptides and immunogloublins. Nature 308:153–158PubMedCrossRefGoogle Scholar
  13. Hood L, Kronenberg M, Hunkapiller T (1985) T cell antigen receptors and the immunoglobulin super gene family. Cell 40:225–229PubMedCrossRefGoogle Scholar
  14. Huppi K, Jouvin-Marche E, Scott C, Potter M, Weigert M (1985) Genetic polymorphism at the K chain locus in mice: comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics 21:445–457PubMedCrossRefGoogle Scholar
  15. Huppi K, Jouvin-Marche E, D’Hoostelaere LA, Mushinski JF, Rudikoff S (1985) T cell receptor α and β gene diversity among mouse species (Submitted)Google Scholar
  16. Ikuta K, Ogura T, Shimizu A, Honjo T (1985) Low frequency of somatic mutation in β chain variable region genes of human T-cell receptors. Proc Natl Acad Sci (USA) 82:7701–7705CrossRefGoogle Scholar
  17. Kotzin BL, Barr VL, Palmer E (1985) A large deletion within the T-cell receptor beta-chain gene complex in New Zealand white mice. Science 229:167–170PubMedCrossRefGoogle Scholar
  18. Sim GK, Augustin AA (1985) V3 gene polymorphism and a major polyclonal T cell receptor idiotype. Cell 42:89–92PubMedCrossRefGoogle Scholar
  19. Tonegawa S (1983) Somatic generation of antibody diversity. Nature 302:575–581PubMedCrossRefGoogle Scholar
  20. Valbuena O, Marcu KB, Weigert M, Perry RP (1978) Multiplicity of germline genes specifying a group of related mouse K chains with implications for the generation of immunoglobulin diversity. Nature 276:780–784PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • K. Huppi
  • L. A. D’Hoostelaere
  • E. Jouvin-Marche

There are no affiliations available

Personalised recommendations