Igk Polymorphism in M. musculus domesticus Populations from Maryland and Delaware

  • L. A. D’Hoostelaere
  • M. Potter
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 127)


The genetic locus that codes for Igk light chains in mice is located on chromosome 6 approximately 32 centimorgans from the centromere (Valbuena et al., 1978; Hengartner et al., 1978; Swan et al., 1979; Gibson et al., 1983; Gibson et al., 1984; Gibson and MacLean 1979; D’Hoostelaere et al., 1985). The number of germline sequences for variable kappa (VK) in inbred mice has been estimated to be from 90 to 320 (Cory et al., 1981; Zeelon et al., 1981; Briles and Carroll 1981; Adams et al., 1981; Potter et al., 1982; Gibson 1984; Nishi et al., 1985). Cory et al., (1981) made their estimates on the basis of 7 canoni cal probes (11 originating from 8 myeloma cell lines). Each canonical DNA probe hybridized with a unique set of VK restriction endonuclease fragments (REFs). The sum of these sets of related VK genes are thought to correspond to a large part but not all of the VK groups identified by partial and complete amino acid sequencing (Potter et al., 1982). Some of the sets are clusters of nearest neighbor genes, e.g., as with VK21 (Heinrich et al., 1984). Clustering must be demonstrated for each canonical set since human VK groups have been found to be interspersed (Jaenichem et al., 1984; Peck et al., 1985).


Inbred Mouse Dark Agouti Complete Amino Acid Sequencing Trap Night Tobacco Farm 
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  1. Adams JM, Kemp DJ, Bernard O, Gough N, Webb E, Tyler B, Gerondakis S, Cory S (1981) Organization and expression of murine immunoglobulin genes. Immunol Reviews 59:5–32CrossRefGoogle Scholar
  2. Briles DE, Carroll RJ (1981) A simple method for estimating the probable number of different antibodies by examining the repeat frequencies of sequences or isoelectric focusing patterns. Mol Immunol 18:29–38PubMedCrossRefGoogle Scholar
  3. Coleclough C, Perry RP, Karjalainen K, Weigert M (1981) Aberrant rearrangements contribute significantly to the allelic exclusion of immunoglobulin gene expression. Nature 290:372–378PubMedCrossRefGoogle Scholar
  4. Cory S, Tyler BM, Adams JM (1981) Sets of immunoglobulin VK genes homologous to ten cloned VK sequences: implications for the number of germline VK genes. J Mol Appl Genet 1:103–116PubMedGoogle Scholar
  5. D’Hoostelaere LA, Jouvin-Marche E, Huppi K (1985) Localization of CTβ and CK on murine chromosome six. Immunogenetics 22:277–283PubMedCrossRefGoogle Scholar
  6. Fischer-Lindahl K (Submitted) Genetic variants of histocompatibility antigens from wild mice. (this edition)Google Scholar
  7. Gibson DM (1984) Evidence for 65 electrophoretically distinct groups of light chains in BALB/c and NZB myelomas. Mol Immunol 21:421–432PubMedCrossRefGoogle Scholar
  8. Gibson DM, MacLean SJ (1979) Ef-2: A new Ly-3-linked light-chain marker expressed in normal mouse serum immunogloublin. J Exp Med 149: 1477–1486PubMedCrossRefGoogle Scholar
  9. Gibson DM, MacLean SJ, Anctil D, Mathieson BJ (1984) Recombination between kappa chain genetic markers in the mouse. Immunogenetics 20:493–501PubMedCrossRefGoogle Scholar
  10. Gibson DM, MacLean SJ, Cherry M (1983) Recombination between kappa chain genetic markers and the Lyt-3 locus. Immunogenetics 18: 111–116PubMedCrossRefGoogle Scholar
  11. Heinrich G, Traunecher A, Tonegawa S (1984) Somatic mutation creates diversity in the major group of mouse immunoglobulin K light chains. J Exp Med 159:417–435PubMedCrossRefGoogle Scholar
  12. Hengartner H, Meo T, Mullen E (1978) Assignment of genes for immunoglobulin K and heavy chains to chromosome 6 and 12 in mouse. Proc Natl Acad Sci USA 75:4494–4498PubMedCrossRefGoogle Scholar
  13. Huppi K, Jouvin-Marche E, Scott C, Potter M, Weigert M (1985) Genetic polymorphism at the K chain locus in mice: comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics 21:445–457PubMedCrossRefGoogle Scholar
  14. Jaenichen H, Peck M, Lindenmaier W, Wildgruber N, Zachau H (1984) Composite human VK genes and a model of their evolution. Nucl Acid Res 12:5249–5263CrossRefGoogle Scholar
  15. Nishi M, Kataoka T, Honjo T (1985) Preferential rearrangement of immunoglobulin K chain joining region JK1 and JK2 segments in mouse spleen DNA. Proc Natl Acad Sci USA 82:6399–6403PubMedCrossRefGoogle Scholar
  16. Peck M, Smola H, Pohlenz H, Straubinger B, Gerl R, Zachau H (1985) A large section of the gene locus encoding human immunoglobulin variable regions of the kappa type is duplicated. J Mol Biol 183: 291–299CrossRefGoogle Scholar
  17. Perry RR (unpublished) A non-productively rearranged VK11 by DNA sequencing. p6684K- is a 4 kbp Hind III fragment. Perry R, ICR, Phi1adelphiaGoogle Scholar
  18. Potter M, Newell JB, Rudikoff S, Haber E (1982) Classification of mouse VK groups based on the partial amino acid sequence to the first invariant tryptophan: impact of 14 new sequences from IgG myeloma proteins. Mol Immunol 19:1619–1630PubMedCrossRefGoogle Scholar
  19. Rowe FP, Taylor EJ, Chudley AHJ (1963) The numbers and movements of house-mice (Mus musculus L.) in the vicinity of four corn-ricks. J Anim Ecol 32:87–97CrossRefGoogle Scholar
  20. Rudikoff S, Jouvin-Marche E (submitted) Evolution of CK and VK genes in wild mice. (this edition)Google Scholar
  21. Seising E, Storb U (1981) Somatic mutation of immunoglobulin lightchain variable region genes. Cell 25:47–58CrossRefGoogle Scholar
  22. Shapiro M (unpublished) A germline VK10 by DNA sequencing. pC3386 is a 0.9 kbp Eco RI-Hind III fragment. Shapiro M, ICR, Phi1adelphiaGoogle Scholar
  23. Shiroishi T, Sagai T, Moriwaki K (1982) A new wild-derived H-2 haplotype enhancing K-IA recombination. Nature 300:370–372PubMedCrossRefGoogle Scholar
  24. Stafford J, Queen C (1983) Cell-type specific expression of a transfected immunoglobulin gene. Nature 306:77–79PubMedCrossRefGoogle Scholar
  25. Steinmetz M (submitted) Polymorphism and recombination hot spots in murine MHC (this edition)Google Scholar
  26. Swan D, D’Eustachio P, Leinwand L, Seidman J, Keithley D, Ruddle FH (1979) Chromosomal assignment of the mouse K light chain genes. Proc Natl Acad Sci USA 76:2735–2739PubMedCrossRefGoogle Scholar
  27. Valbuena O, Marcu KB, Croce CM, Huebner K, Weigert M, Perry RP (1978) Chromosomal location of immunoglobulin genes. Proc Natl Acad Sci USA 75:2883–2887PubMedCrossRefGoogle Scholar
  28. Zeelon EP, Bothwell ALM, Kantor F, Schechter I (1981) An experimental approach to enumerate the genes coding for immunoglobulin variable region. Nucl Acid Res 9:3809–3820CrossRefGoogle Scholar

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© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • L. A. D’Hoostelaere
  • M. Potter

There are no affiliations available

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