Abstract
Electrophoretic methods of various kinds are used to reveal genetically-encoded variations in enzymes and other proteins. The variants that are detected provide invaluable genetic markers applicable in a variety of kinds of biological and biomedical research. It is recognized, however, that many genetically-determined differences in amino acid sequence go undetected in standard electrophoretic analyses. These “silent” changes involve substitutions of one neutral amino acid for another neutral amino acid (alanine, glycine, valine, isoleucine, leucine, serine, threonine, asparagine, glutamine).
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© 1986 Springer-Verlag Berlin · Heidelberg
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Whitney, J.B. (1986). Immobilized-gradient Isoelectric Focusing: Detection of “Silent” Biochemical Genetic Variants. In: Potter, M., Nadeau, J.H., Cancro, M.P. (eds) The Wild Mouse in Immunology. Current Topics in Microbiology and Immunology, vol 127. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71304-0_16
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DOI: https://doi.org/10.1007/978-3-642-71304-0_16
Publisher Name: Springer, Berlin, Heidelberg
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