Skip to main content
SpringerLink
Log in

Immobilized-gradient Isoelectric Focusing: Detection of “Silent” Biochemical Genetic Variants

  • Conference paper
Book cover The Wild Mouse in Immunology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 127))

  • 126 Accesses

Abstract

Electrophoretic methods of various kinds are used to reveal genetically-encoded variations in enzymes and other proteins. The variants that are detected provide invaluable genetic markers applicable in a variety of kinds of biological and biomedical research. It is recognized, however, that many genetically-determined differences in amino acid sequence go undetected in standard electrophoretic analyses. These “silent” changes involve substitutions of one neutral amino acid for another neutral amino acid (alanine, glycine, valine, isoleucine, leucine, serine, threonine, asparagine, glutamine).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Allen RC, Saravis CA, Maurer HR (1984) Gel electrophoresis and isoelectric focusing of proteins: selected techniques. de Gruyter, New York, 255 pp

    Google Scholar 

  • Lewontin RC (1974) The genetic basis of evolutionary change. Columbia University Press, 346 pp

    Google Scholar 

  • Modiano G, Battistuzzi G, Motulsky AG (1981) nonrandom patterns of codon usage and of nucleotide substitutions in human alpha- and beta-globin genes: An evolutionary strategy reducing the rate of mutations with drastic effects? Proc Natl Acad Sci 78:1110–1114

    Article  PubMed  CAS  Google Scholar 

  • Popp RA, Bailiff EG, Skow LC, Whitney III JB (1982) The primary structure of genetic variants of mouse hemoglobin. Biochem Genet 20: 199–208

    Article  PubMed  CAS  Google Scholar 

  • Potter M (1981) Wild mice. Mouse News Letter 64:62–64.

    Google Scholar 

  • Righetti PG (1983) Isoelectric focusing: Theory, methodology, and applications. Elsevier Biomedical, New York, 386 pp

    Google Scholar 

  • Staats J (1980) Standardized nomenclature for inbred strains of mice: Seventh listing. Cancer Res 40:2083–2128

    PubMed  CAS  Google Scholar 

  • Whitney III JB, Copland GT, Skow LC, Russell ES (1979) Resolution of the products of the duplicated alpha-chain loci by isoelectric focusing. Proc Natl Acad Sci 76:867–871

    Article  PubMed  CAS  Google Scholar 

  • Whitney III JB, Cobb RR, Popp RA, O’Rourke TW (1985) Detection of neutral amino acid substitutions in proteins. Proc Natl Acad Sci 82 (in press)

    Google Scholar 

Download references

Authors
  1. J. B. Whitney III
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of Genetics, National Institutes of Health, National Cancer Institute, 20205, Bethesda, MD, USA

    Michael Potter M.D.

  2. The Jackson Laboratory, 04609, Bar Harbor, ME, USA

    Joseph H. Nadeau Ph.D.

  3. School of Medicine, Department of Pathology, University of Pennsylvania, 36th Street and Hamilton Walk, 19104, Philadelphia, PA, USA

    Michael P. Cancro Ph.D.

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Springer-Verlag Berlin · Heidelberg

About this paper

Cite this paper

Whitney, J.B. (1986). Immobilized-gradient Isoelectric Focusing: Detection of “Silent” Biochemical Genetic Variants. In: Potter, M., Nadeau, J.H., Cancro, M.P. (eds) The Wild Mouse in Immunology. Current Topics in Microbiology and Immunology, vol 127. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71304-0_16

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71304-0_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71306-4

  • Online ISBN: 978-3-642-71304-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation