Advertisement

Genetic Variation in Major Urinary Proteins in Wild Mice

  • W. A. Held
  • B. M. Sampsell
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 127)

Abstract

The major urinary proteins are a group of closely-related polypeptides of low molecular weight (17,800) and acidic isoelectric points. As their name indicates, collectively they represent the predominant protein fraction of mouse urine, and in male mice may be excreted at the rate of 20 mg per day (Szoka and Paigen 1978). These urinary proteins are synthesized in the liver where MUP mRNA accounts for 5% of the total messenger RNA (Hastie et al. 1979). Inbred male mice excrete about 20 times more MUP than females and have about five-fold higher MUP mRNA levels in the liver. MUP mRNA has also been detected in the submaxillary, lachrymal, and mammary glands (Shaw et al. 1983). The function of MUPs and whether they appear in other body secretions besides urine is not known. The genes coding for the MUPs are members of a multigene family with approximately 30–35 copies (Hastie et al. 1979; Bishop et al. 1982). The gene cluster has been mapped to chromosome 4 and is linked to the brown locus (Bennett et al. 1982; Krauter et al. 1982; Bishop et al. 1982). Recent work (Clark et al. 1984) indicates that many of the MUP genes on chromosome 4 are organized pairwise in a divergent fashion with each pair of consisting of about 45 kb of DNA.

Keywords

Inbred Strain Multigene Family P199 Sequence Body Secretion Mouse Urine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bennett KL, Lalley PA, Barth RK, Hastie ND (1982) Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids, Proc Natl Acad Sci 79:1220PubMedCrossRefGoogle Scholar
  2. Bishop JO, Clark AJ, Clissold PM, Hainey S, Franke U (1982) Two main groups of mouse major urinary protein genes, both largely located on chromosome 4.. EMBO J 1:615–620PubMedGoogle Scholar
  3. Clark AJ, Hickman J, Bishop J (1984) A 45-kb DNA domain with two divergently oriented genes is the unit of organization of the murine major urinary protein genes. EMBO J 3:2055–2064PubMedGoogle Scholar
  4. Clissold PM, Bishop JO (1981) Molecular cloning of cDNA sequences transcribed from mouse liver endoplasmic reticulum poly (A) mRNA. Gene 15:225–235PubMedCrossRefGoogle Scholar
  5. Finlayson JS, Potter M, Runner RC (1963) Electrophoretic variation and sex dimorphism of the major urinary protein complex in inbred mice: a new genetic marker. J Natl Cancer Inst 31:91–97PubMedGoogle Scholar
  6. Groen A, Lagerwerf AJ (1979) Genetically determined electrophoretic variants of the major urinary protein (Mup) complex in mouse urine. Anim Blood Grps Biochem Genet 10:107–114CrossRefGoogle Scholar
  7. Hastie ND, Held WA, Toole JJ (1979) Multiple genes coding for the androgen-regulated major urinary proteins of the mouse. Cell 17:449–457PubMedCrossRefGoogle Scholar
  8. Hudson DM, Finlayson JS, Potter M (1967) Linkage of one component of the major urinary protein complex of mice to the brown coat color locus. Genet Res 10:195–198PubMedCrossRefGoogle Scholar
  9. Knopf JL, Gallagher JF, Held WA (1983) Differential, multi-hormonal regulation of the mouse major urinary protein gene family in the liver. Mol Cell Biol 3:2232–2240PubMedGoogle Scholar
  10. Krauter K, Leinwand L, Dfeustachio P, Ruddle F, Darnell JE, Jr (1982) Structural genes of the mouse major urinary protein are on chromosome 4. J Cell Biol 94:414–417PubMedCrossRefGoogle Scholar
  11. Kuhn NJ, Woodworth M, Gross KW, Held WA (1984) Subfamilies of the mouse major urinary protein (MUP) multi-gene family: sequence analysis of cDNA clones and differential regulation in the liver. Nucleic Acids Res 12:6073–6090PubMedCrossRefGoogle Scholar
  12. Sampseil B, Held WA (1985) Variation in the major urinary protein multigene family in wild-derived mice. Genetics 109:54 9–568Google Scholar
  13. Shaw PH, Held WA, Hastie ND (1983) The gene family for major urinary proteins: expression in several secretory tissues of the mouse. Cell 32:755–761PubMedCrossRefGoogle Scholar
  14. Szoka PR, Paigen K (1978) Regulation of mouse major urinary protein by the Mup-a gene. Genetics 90:597–612PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • W. A. Held
  • B. M. Sampsell

There are no affiliations available

Personalised recommendations