Alleles of β-Glucuronidase Found in Wild Mice
β-Glucuronidase (Gus) has been extensively studied in the mouse as a model of mammalian gene regulation. The structural gene maps to chromosome 5 and variation for the regulation of Gus gene expression cosegregates with the structural locus. The regulatory phenotypes include variation in systemic levels of gene expression (Pfister et al., 1985), variation in testosterone induction of kidney β-glucuronidase (Palmer et al., 1983; Swank et al., 1973) and variation in Gus expression within individual organs. The various structural and regulatory phenotypes which cosegregate at the GUS locus are designated as haplotypes [Gus] and the different regulatory phenotypes have been designated as separate elements of the haplotype complex (Table 1) (Paigen, 1979).
KeywordsRecombinant Inbred Congenic Strain Heat Inactivation Enzyme Structure Testosterone Treatment
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- Elliott RW, Hohman C, Pazik J, Gallagher PM, Palmer R, Ganschow RE (1985) DNA polymorphisms correlate with glucuronidase haplotypes. J Biol Chem (submitted)Google Scholar
- Medda, S., von Deimling O, Swank RT (1985) Identity of esterase-22 and egasyn, the protein which complexes with microsomal β-glucuronidase. (submitted)Google Scholar
- Pfister K, Chapman V, Watson G, Paigen K (1985) Genetic variation for enzyme structure and systemic regulation in two new haplotypes of the β-glucuronidase gene of Mus musculus castancus. J Biol Chem (in press)Google Scholar
- Swank RT, Paigen K, Davey R, Chapman V, Labarca C, Watson G, Ganschow R, Brandt EJ, Novak EK (1978) Genetic regulation of mammalian glucuronidase. Recent Progress in Hormone Res 34:401–436Google Scholar