Effect of Ochratoxin A on Enzyme Activities and Macromolecules Synthesis in MDCK Cells

  • E. E. Creppy
  • A. Kane
  • E. Giessen-Crouse
  • A. Roth
  • R. Röschenthaler
  • G. Dirheimer
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

Protein, RNA, and DNA synthesis, and enzyme (γGT, LDH, LAP, Al-P, NAG) activities were assayed in Madin Darby Canine Kidney cells (MDCK) treated by ochratoxin A, a mycotoxin known to be nephrotoxic in animal and man. Both cellular macromolecules syntheses and enzymatic activities are inhibited by OTA in a dose-dependent manner after 24 h incubation. The protection by 100 µM additional phenylalanine is optimal when MDCK cells were pretreated 4 h before the poisoning by OTA. When OTA 25 µM and phenylalanine 100 µM were added simultaneously the prevention of toxic effects is evident, but not complete in spite of the respective intracellular concentrations of OTA 2.22 µM and phenylalanine 2.4 µM which are normally not in favor of a strong inhibition by OTA, except if one admits that MDCK cells are really very sensitive to OTA.

Key words

Ochratoxin A Madin darby canine kidney cells Enzyme activity Macromolecule synthesis Phenylalanine 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • E. E. Creppy
    • 1
    • 2
  • A. Kane
    • 2
  • E. Giessen-Crouse
    • 3
  • A. Roth
    • 4
  • R. Röschenthaler
    • 4
  • G. Dirheimer
    • 1
    • 2
  1. 1.Institut de Biologie Moléculaire et Cellulaire du CNRSUniversité Louis PasteurStrasbourgFrance
  2. 2.Faculté de PharmacieUniversité Louis PasteurStrasbourgFrance
  3. 3.Institut de PharmacologieFaculté de MédecineStrasbourgFrance
  4. 4.Institut für MikrobiologieUniversität MünsterMünsterGermany

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