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A Rapid Method for Quantitation of Toxic Heart Dilatation in Rats

  • A. Härtl
  • J. Güttner
  • R. Stracke
  • H. Hoffmann
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

Several methods are available for evaluating the cardiotoxic potency of anthracycline antitumor antibiotics (Doroshow et al. 1979). Although the Zbinden rat model is frequently used (Zbinden et al. 1978), it should be completed by morphological evaluation. However, electron microscopy is too expensive for drug screening. Thus, in this study the quantitation of the heart dilatation, as being a simple and rapidly detectable parameter for assessment of heart toxicity, is proposed. In rats the cardiotoxic effect of repeated i.v. doses of an anthracycline derivative of the rhodomycinone type was studied by the gravimetric determination of the post mortem filling volume of the heart (Fig. 1). In addition, the ECG changes (QRS complex) during the treatment period as well as ultrastructural signs of myocardiac damage (volume fractions of mitochondria, of normal and damaged myofibrils and of perimitochondrial edema) were detected. The results show a close correlation of the alteration of both the functional and the morphometric parameters. Moreover, the findings suggest that the post mortem intracardiac blood volume seems to be highly sensitive and a simple parameter for anthracycline induced heart dilatation in rodents.

Key words

Cardiotoxicity Postmortem intracardiac blood volume Rat Anthracycline 

References

  1. Doroshow JH, Locker GY, Myers GE (1978) Experimental animal models of adriamycin cardiotoxicity. Cancer Treat Rep 63: 855–860Google Scholar
  2. Ferrans VJ (1982) Morphologic assessment of cardiac lesions caused by anthracyclines. Dev Oncol 10: 331–347Google Scholar
  3. Hoffmann H, Härtl A, Amlacher R, Güttner J, Fritsch RS (1983) Kardiotoxische und myelosuppressive Aktivitäten einiger Antitumor-Antibiotika vom Anthrazyklintyp bei Ratten. Arch. Geschwulstforsch. 53: 429–439PubMedGoogle Scholar
  4. Zbinden G, Bachmann E, Holderegger C (1978) Model systems for cardiotoxic effects of anthracyclines. Antibiotics Chemother 23: 255–270Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • A. Härtl
    • 1
  • J. Güttner
    • 1
  • R. Stracke
    • 1
  • H. Hoffmann
    • 1
  1. 1.Central Institute of Microbiology and Experimental TherapyAcademy of Sciences of the GDRJenaGDR

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