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Carcinogenicity Testing in Nude Mouse Cell Cultures

  • A. M. Laaksonen
  • R. A. Mäntyjärvi
  • O. O. P. Hänninen
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

Secondary cultures of newborn NMRI nu/nu (nude) mouse skin fibroblasts were used as targets for transformation by the combined administration of SV40 and 3-methylcholanthrene (MC). The long (72 h) post-treatment with MC increased virus transformation as much as 4.3-fold. In contrast, anthracene, a non-carcinogenic coumpound, had no effect on viral transformation frequency. Despite considerable variation within a group, the cell lines transformed by the combination treatment, as a group, were more tumourigenic than cell lines transformed by SV40 alone.

Key words

Nude mouse SV40 3-Methylcholanthrene Cocarcinogenesis Transformed cell lines 

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References

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • A. M. Laaksonen
    • 1
  • R. A. Mäntyjärvi
    • 1
  • O. O. P. Hänninen
    • 1
  1. 1.Department of Clinical Microbiology and Department of PhysiologyUniversity of KuopioKuopioFinland

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