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Development of T Lymphocytes Within the Thymus and Within Thymic Nurse Cells

  • K. Shortman
  • R. Scollay
  • P. Andrews
  • R. Boyd
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 126)

Abstract

Any detailed study of T-cell development within the thymus requires, as a first step, the ability of distinguish the subpopulations of lymphoid cells which may be steps along a developmental pathway. About 95% of adult murine thymic lymphocytes can be assigned to one of four discrete subpopulations as summarized in Table 1. These major subpopulations, and many of their surface markers, have been recognized for some time, and the recent application of multiparameter flow cytometric analysis (Scollay and Shortman 1983) has merely served to emphasize how clear cut this four-way division can be. This subdivision is obtained as follows.

Keywords

Nurse Cell Medullary Cell Mouse Thymus Murine Thymocyte Cortical Thymocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andrews P, Boyd R (1985) The murine thymic nurse cell: an isolated thymic microenvironment. Eur J Immunol 15: 36–42PubMedCrossRefGoogle Scholar
  2. Andrews P, Shortman K, Scollay R, Potworowski EF, Kruisbeek AM, Goldstein G, Trainin N, Bach J-F (1985) Thymic hormones do not induce proliferative ability or cytolytic function in PNA+ cortical thymocytes. Cell Immunol 91: 455–466PubMedCrossRefGoogle Scholar
  3. Borum K (1973) Cell kinetics in mouse thymus studied by simultaneous use of 3H-thymidine and colchicine. Cell Tissue Kinet 6: 545–552PubMedGoogle Scholar
  4. Ceredig R, Glasebrook AL, MacDonald HR (1982) Phenotypic and functional properties of murine thymocytes. I. Precursors of cytolytic T lymphocytes and interleukin-2 producing cells are con¬tained within a subpopulation of “mature” thymocytes as analysed by monoclonal antibodies and flow microfluorometry. J Exp Med 155: 358–379PubMedCrossRefGoogle Scholar
  5. Ceredig R, Dialynas DP, Fitch FW, MacDonald HR (1983a) Precursors of T-cell growth factor pro¬ducing cells in the thymus: ontogeny, frequency and quantitative recovery in a subpopulation of phenotypically mature thymocytes defined by monoclonal antibody GK-1.5. J Exp Med 158: 1654–1671PubMedCrossRefGoogle Scholar
  6. Ceredig R, Sekaly RP, MacDonald HR (1983b) Differentiation in vitro of Lyt-2+ thymocytes from embryonic Lyt-2b precursors. Nature 303: 248 - 250PubMedCrossRefGoogle Scholar
  7. Chen W-F, Scollay R, Shortman K (1982) The functional capacity of thymus subpopulations: limit- dilution analysis of all precursors of cytotoxic lymphocytes and of all T cells capable of prolifera¬tion in subpopulations separated by the use of peanut agglutinin. J Immunol 129: 18–24Google Scholar
  8. Chen W-F, Scollay R, Clark-Lewis I, Shortman K (1983a) The size of functional T-lymphocyte pools within thymic medullary and cortical cell subsets. Thymus 5: 179–195PubMedGoogle Scholar
  9. Chen W-F, Scollay R, Shortman K (1983b) The Ly phenotype of functional medullary thymocytes. Thymus 5: 197–207PubMedGoogle Scholar
  10. Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1-lt;265; expression on L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev 74: 29–56PubMedCrossRefGoogle Scholar
  11. Elliot EV (1973) A persistent lymphoid population in the thymus. Nature New Biol 242: 150–152Google Scholar
  12. Fathman CG, Small M, Herzenberg LA, Weissman IL (1975) Thymus cell maturation. II. Differ¬entiation of three “mature” subclasses in vivo. Cell Immunol 15: 109–128PubMedCrossRefGoogle Scholar
  13. Fink PJ, Weissman IL, Kaplan HS, Kyewski BA (1984) The immunocompetence of murine stromal cell-associated thymocytes. J Immunol 132: 2266–2272PubMedGoogle Scholar
  14. Fowlkes B-J, Edison L, Mathieson B, Chused TM (1985) In: U. C. L. A. Symposium, Regulation of the immune system, (to be published) Irle C, Piguet P-F, Vassalli P (1978) In vitro maturation of immature thymocytes into immunocompetent T cells in the absence of direct thymic influence. J Exp Med 148:32–45Google Scholar
  15. Joel DD, Chanana AD, Cottier H, Cronkite EP, Laissue J A (1977) Fate of thymocytes: studies with 125I-iododeoxyuridine and 3H-thymidine in mice. Cell Tissue Kinet 10: 57–69PubMedGoogle Scholar
  16. Kisielow P, von Boehmer H, Haas W (1982) Functional and phenotypic properties of subpopulations of murine thymocytes. I. The bulk of peanut agglutinin-positive Lyt-1, 2, 3 thymocytes lacks pre¬cursors of cytotoxic T lymphocytes responsive to interleukin 2 ( T cell growth factor ). Eur J Immunol 12: 463–467PubMedCrossRefGoogle Scholar
  17. Kyewski RA, Kaplan HS (1982) Lymphoepithelial interactions in the mouse thymus: phenotypic and kinetic studies on thymic nurse cells. J Immunol 128: 2287–2294PubMedGoogle Scholar
  18. McPhee D, Pye S, Shortman K (1979) The differentiation of Tlymphocytes. V. Evidence for intrathymic death of most thymocytes. Thymus 1: 151–161PubMedGoogle Scholar
  19. Metcalf D (1966) The nature and regulation of lymphopoiesis in normal and neoplastic thymus. In: Wolstenholme GWE, Porter R (eds) The thymus: experimental and clinical studies, CIBA Foun¬dation Symposium, Churchill, London, p. 242Google Scholar
  20. Naparstek Y, Holoshitz J, Eisenstein S, Reshef T, Rappaport S, Chemke J, Ben-Nun A, Cohen IR (1982) Effector T lymphocyte line cells migrate to the thymus and persist there. Nature 300: 262–264PubMedCrossRefGoogle Scholar
  21. Owen JJT, Raff MC (1980) Studies on the differentiation of thymus-derived lymphocytes. J Exp Med 132: 1216–1232CrossRefGoogle Scholar
  22. Raff MC (1971) Evidence for a subpopulation of mature lymphocytes within the mouse thymus. Nature New Biol 229: 182–183PubMedGoogle Scholar
  23. Reichert RA, Gallatin WM, Butcher EC, Weissman IL (1984) A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes. Cell 38: 89–99PubMedCrossRefGoogle Scholar
  24. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF (1980) Discrete stages of human intrathymic differentiation. Analysis of normal thymocytes and leukemic lymphoblasts of T cell lineage. Proc Natl Acad Sci USA 77: 1588–1592Google Scholar
  25. Scollay R (1982) Thymus cell migration: cells migrating from the thymus to peripheral lymphoid organs have a “mature” phenotype. J Immunol 128: 1566–1570PubMedGoogle Scholar
  26. Scollay R (1984) The long-lived medullary thymocyte re-visited: precise quantitation of a very small subset. J Immunol 132:1085–1089 Scollay R, Shortman K (1983) Thymocyte subpopulations: an experimental review, including flow cytometric cross-correlations between the major murine thymocyte markers. Thymus 5: 245–295Google Scholar
  27. Scollay R, Shortman K (1985) Identification of early stages of T lymphocyte development in the thy¬mus cortex and medulla. J Immunol 134: 3632–3642PubMedGoogle Scholar
  28. Scollay R, Kocken M, Butcher E, Weissman I (1978) Lyt markers and thymus cell migrants. Nature 276: 79–80PubMedCrossRefGoogle Scholar
  29. Scollay R, Butcher E, Weissman I (1980a) Thymus migration: quantitative studies on the rate of migration of cells from the thymus to the periphery in mice. Eur J Immunol 10: 210–218PubMedCrossRefGoogle Scholar
  30. Scollay R, Jacobs S, Jerabek L, Butcher E, Weissman IL (1980b) T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens. J Immunol 124: 2845–2853PubMedGoogle Scholar
  31. Scollay R, Bartlett P, Shortman K (1984a) T cell development in the adult murine thymus: Changes in the expression of the surface antigens Ly 2, L3T4 and B2A2 during development from early precursor cells to emigrants. Immunol Rev 82: 79PubMedCrossRefGoogle Scholar
  32. Scollay R, Wilson A, Shortman K (1984b) Thymus cell migration: analysis of thymus emigrants with markers that distinguish medullary thymocytes from peripheral T cells. J Immunol 132: 1089–1094PubMedGoogle Scholar
  33. Scollay R, Chen W-F, Shortman K (1984c) The functional capabilities of cells leaving the thymus. J Immunol 132: 25–30PubMedGoogle Scholar
  34. Shortman K, Jackson H (1974) The differentiation of T-lymphocytes. I. Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells. Cell Immunol 12: 230–246PubMedCrossRefGoogle Scholar
  35. Shortman K, Mandel T, Andrews P, Scollay R (1985) Are any functionally mature cells of medullary phenotype located in the thymus cortex? Cell Immunol 93: 350–363PubMedCrossRefGoogle Scholar
  36. Vakharia DD (1983) Demonstration of keratin filaments in thymic nurse cells (TNC) and alloreactivity of TNC-T cell population. Thymus 5: 43–52PubMedGoogle Scholar
  37. Vakharia DD, Mitchison NA (1984) Helper T cell activity demonstrated by thymic nurse cell T cells ( TNC-T ). Immunol 51: 269–273Google Scholar
  38. Weissman IL (1973) Thymus cell maturation. Studies on the origin of cortisone resistant thymic lymphocytes. J Exp Med 137: 504–510PubMedCrossRefGoogle Scholar
  39. Wekerle H, Ketelsen UP (1980) Thymic nurse cells - la bearing epithelium involved in T-lymphocyte differentiation. Nature 283: 402–404PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • K. Shortman
    • 1
  • R. Scollay
    • 1
  • P. Andrews
    • 1
  • R. Boyd
    • 2
  1. 1.Royal Melbourne HospitalWalter and Eliza Hall Institute of Medical Research, Post OfficeAustralia
  2. 2.Department of Pathology and ImmunologyMonash University Medical SchoolMelbourneAustralia

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