Abstract
With the advent of recombinant DNA techniques it soon became clear that eukaryotic genes usually are organized in exons and introns. The polymorphic variants of serum proteins and of other immunogenetic systems which we hitherto study are, of course, ascribable to nucleotide variation in the corresponding exons. The introns are not expressed, but are cut out in messenger RNA production. Hybridization between messenger RNA and single-stranded DNA demonstrates that very substantial parts of DNA are not translated (see Chambon 1981). For a majority of genes the base pairs of the introns is twice or more the base number of the exons (see for example Efstratiadis et al 1980). In addition, the base pair composition of the introns commonly vary more than those of the exons (see Milner-White 1984). DNA hybridization techniques thus give access to a new and abundantly rich source of polymorphisms: Study the variation of allelic restriction fragments of numerous introns! As an example Krontiris and coworkers (1984) studied the allelic restriction fragments of the oncogene Harvey-ras.
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Grubb, R. (1986). Recent Research on Genetic Regulation and Function of Some Serum Proteins. In: Brinkmann, B., Henningsen, K. (eds) 11th Congress of the Society for Forensic Haemogenetics (Gesellschaft für forensische Blutgruppenkunde e.V.). Advances in Forensic Haemogenetics, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71150-3_9
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