Abstract
We have developed a procedure for the isolation of sperm DNA from semen samples collected after sexual activity. The use of a differential lysis procedure allows the recovery of sperm free of female cells. DNA was purified from these samples, digested with particular restriction endonucleases, fractionated by agarose gel electrophoresis, and hybridized with specific recombinant DNA probes that recognize two highly polymorphic DNA sequences. DNA was purified from nine semen samples, recovered from the female partners of volunteer couples, and the sizes of the polymorphic DNA fragments were measured. These sizes were compared with those obtained from DNA purified from the blood of the male and female sexual partners. In all cases, the pattern of DNA polymorphisms obtained from the semen samples was identical to that of DNA purified from blood of the male sexual partner. This technique is applicable to the identification of an assailant in rape cases.
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© 1986 Springer-Verlag Berlin Heidelberg
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Baird, M., Giusti, A., Balazs, I., Glassberg, J. (1986). Application of DNA Polymorphisms to the Forensic Examination of Semen. In: Brinkmann, B., Henningsen, K. (eds) 11th Congress of the Society for Forensic Haemogenetics (Gesellschaft für forensische Blutgruppenkunde e.V.). Advances in Forensic Haemogenetics, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71150-3_77
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DOI: https://doi.org/10.1007/978-3-642-71150-3_77
Publisher Name: Springer, Berlin, Heidelberg
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