Simple Method for Haptoglobin Subtyping

  • D. Patzelt
  • H. Schröder
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 1)

Abstract

Hardly any forensic use is being made of the informative Hp subtype polymorphism because of the need for Hp purification which so far has been considered time-consuming. The introduction to forensic serology of highly sensitive iso-electric focusing encouraged some rethinking of the problem of Hp preparation. Proceeding from existing experience, we have succeeded in developing a practicable method which meets the demands on paternity assessment.

Keywords

Cellulose Formaldehyde Starch Iodine Amide 

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References

  1. 1.
    Patzelt, D., and H. Schröder: Haptoglobin subtypes in Berlin, GDR. A simple procedure for haptoglobin purification and subtyping. Z. Rechtsmedizin (1985) 94: 207–212CrossRefGoogle Scholar
  2. 2.
    Schössler, W., H. Zorn, and D. Patzelt: Ein einfaches Verfahren zur Präparation von Haptoglobin. Acta Biol. Med. Germ. (1979) 38: 669–671Google Scholar
  3. 3.
    Shibata, K., J. Constans, M. Viau, and H. Matsumoto: Polymorphism of the haptoglobin peptides by iso-electric focusing electrophoresis and iso-electric point determination. Hum. Genet. (1982) 61: 210–214PubMedCrossRefGoogle Scholar
  4. 4.
    Smithies, O., G.E. Connell, and G.E. Dixon: Inheritance of haptoglobin subtypes. Amer. J. Hum. Gent. (1962) 14: 14–21Google Scholar
  5. 5.
    Steck, G., D. Leuthard, and R.R. Bürk: Detection of basic proteins and low molecular weight peptides in polyacrylamide gels by formaldehyde fixation. Anal. Biochem. (1980) 107: 21–24PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • D. Patzelt
    • 1
  • H. Schröder
    • 1
  1. 1.Institute of Forensic MedicineHumboldt-UniversitätGDR

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