Abstract
Immunoassays provide rapid and sensitive methods for the quantitative analysis of compounds in complex mixtures. The ability to analyse crude tissue extracts can be attributed to the high specificity of antibody-antigen binding. A high degree of binding specificity does not guarantee a valid assay, however, and substances with weak affinities for the antibody will interfere with antigen binding if present at sufficiently high concentrations. Interference may also arise from nonspecific inhibition, in which case the interfering compound need not show structural similarity to the antigen of interest. Since it is not possible to predict the nature or occurrence of interfering substances, it is important that immunoassays be validated for accuracy.
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© 1986 Springer-Verlag Berlin Heidelberg
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Pengelly, W.L. (1986). Validation of Immunoassays. In: Bopp, M. (eds) Plant Growth Substances 1985. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71018-6_5
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DOI: https://doi.org/10.1007/978-3-642-71018-6_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-71020-9
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