The Effect of Monensin on Receptor-Mediated Endocytosis of Asialofetuin and Secretion of Very Low Density Lipoproteins (VLDL) by Cultured Rat Hepatocytes

  • C. A. Drevon
  • A. C. Rustan
  • J. Ø. Nossen
  • T. Berg
Conference paper


Monensin is a carboxylic ionophore which is able to abolish ionic gradients across membranes (Pressman, 1976). There are indications that monensin inhibits fluid-phase endocytosis (Wilcox et al., 1982), entry of virus particles into fibroblasts and receptor-mediated endocytosis of LDL (Basu et al., 1981) and asialoglycoproteins (Berg et al., 1983). Monensin may cause swelling of the Golgi cisternae and decrease secretion of procollagen, fibronectin (Uchida et al., 1979) and immunoglo-bulins (Tartakoff and Vasalli, 1977). Certain experiments on endocytosis of epidermal growth factor, β-galactosidase, β2-macroglobulin (Pastan and Willing-ham, 1981), insulin (Posner et al., 1982) and asialofetuin (Debanne et al., 1982) suggest that endosomes are directed to the Golgi complex. Conceivably, endo- and exocytosis might have a common pathway through this membrane system.


Golgi Complex Proton Gradient Golgi Cisterna Golgi Function Carboxylic Ionophore 
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  1. 1.
    Basu SK, Goldstein JL, Anderson RGW, Brown MS (1981) Cell 24: 493–502PubMedCrossRefGoogle Scholar
  2. 2.
    Berg T, Blomhoff R, Naess L, Tolleshaug H, Drevon CA (1983) Exp Cell Res 148: 319–330PubMedCrossRefGoogle Scholar
  3. 3.
    Davis RA, Engelhorn SC, Pangburn SH, Weinstein DB (1979) J Biol Chem 254: 2010–2016PubMedGoogle Scholar
  4. 4.
    Debanne MT, Evans WH, Flint N, Regoeczi GE (1982) Nature (London) 298: 398–400CrossRefGoogle Scholar
  5. 5.
    Drevon CA, Engelhorn SC, Steinberg D (1980) J Lipid Res 21: 1065–1071PubMedGoogle Scholar
  6. 6.
    Drevon CA, Tolleshaug H, Carlander B, Berg T (1983) Int J Biochem 15: 827–833PubMedCrossRefGoogle Scholar
  7. 7.
    Glickman J, Croen K, Kelly S, Al-Awqati Q (1983) J Cell Biol 97: 1303–1308PubMedCrossRefGoogle Scholar
  8. 8.
    Harford J, Wolkoff AW, Ashwell G, Klausner RD (1983) J Cell Biol 96: 1824–1828PubMedCrossRefGoogle Scholar
  9. 9.
    Helenius A, Mellman I, Wall D, Hubbard A (1983) Trends Biochem Sci 8: 245–249CrossRefGoogle Scholar
  10. 10.
    Lodish HF, Kong N, Snider M, Strous GJAM (1983) Nature (London) 304: 11–20CrossRefGoogle Scholar
  11. 11.
    Nossen JØ, Rustan AC, Barnard T, Drevon CA (1984) Biochim Biophys Acta 803: 11–20PubMedCrossRefGoogle Scholar
  12. 12.
    Pastan IH, Willingham MC (1981) Science 214: 504–509PubMedCrossRefGoogle Scholar
  13. 13.
    Pedersen PL (1982) in Annals of the New York Academy of Sciences (Carafoli E, Scarpa A, ed) New York Academy of Science New York Vol 402: 1–20Google Scholar
  14. 14.
    Posner BI, Patel BA, Khan MN, Bergeron JJM (1982) J Biol Chem 257: 5789–5799PubMedGoogle Scholar
  15. 15.
    Pressmann BC (1976) Annu Rev Biochem 45: 501–530CrossRefGoogle Scholar
  16. 16.
    Rothman JE (1981) Science 213: 1212–1219PubMedCrossRefGoogle Scholar
  17. 17.
    Rustan AC, Nossen JØ, Berg T, Drevon CA (1985) J Biochem 227: 529–536Google Scholar
  18. 18.
    Tartakoff AM, Vassalli P (1977) J Exp Med 146: 1332–1345PubMedCrossRefGoogle Scholar
  19. 19.
    Uchida N, Smilowitz H, Tanzer ML (1979) Proc Natl Acad Sci (USA) 76: 1868–1872CrossRefGoogle Scholar
  20. 20.
    Wilcox DK, Kitson RP, Widnell CC (1982) J Cell Biol 92: 859–864PubMedCrossRefGoogle Scholar
  21. 21.
    Zhang F, Schneider DL (1983) Biochem Biophys Res Commun 114: 620–625PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • C. A. Drevon
    • 1
  • A. C. Rustan
    • 2
  • J. Ø. Nossen
    • 2
  • T. Berg
    • 3
  1. 1.National Institute of Forensic ToxicologyOsloNorway
  2. 2.Department of PharmacologyInstitute of PharmacyNorway
  3. 3.Institute for Nutrition ResearchUniversity of OsloNorway

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