Synopsis
After removing the alcohol from fragments of preserved specimens of the sponge Suberites domuncula, it is possible to free spicules from the material by digesting it with pepsin or trypsin. Sections cut from such alcohol- preserved specimens which have been embedded in wax are also susceptible to digestion by these enzymes after the tissue has been taken down to water. The hydrolysis leaves intact some slender fibrous connections which unite some of the megascleres at the surface of the sponge.
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© 1987 Springer-Verlag Berlin Heidelberg
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Fry, P.D., Gray, D. (1987). An Enzymatic Technique for the Separation of Spicules from Alcohol-Preserved Sponge Tissue. In: Vacelet, J., Boury-Esnault, N. (eds) Taxonomy of Porifera. NATO ASI Series, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70892-3_5
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DOI: https://doi.org/10.1007/978-3-642-70892-3_5
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