Insecticides as Inhibitors of Respiration

  • J. I. Fukami
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Under aerobic conditions insects meet their energy requirements by respiration. This is essentially the oxidation of biological fuel molecules. Energy is liberated via the respiratory chain and stored as energy-rich phosphate during the process of oxidative phosphorylation. The many chemical steps involved in this process of respiration and in the subsequent conservation of the derived energy in the form of ATP are catalyzed by a number of enzymes. Here we find differences between insects and mammals.


Respiratory Chain Methyl Parathion Flight Muscle Heptachlor Epoxide Succinate Oxidation 
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  1. Anderson AD, March RB, Metealf RL (1954) Inhibition of the succinoxidase system of susceptible and resistant houseflies by DDT and related compounds. Ann Entomol Soc Am 47: 567–595Google Scholar
  2. Barsa MC, Ludwig D (1959) Effects of DDT on the respiratory enzymes of the mealworm, Tenebrio molitor ( L.), and of the housefly, Musca domestica (L.). Ann Entomol Soc Am 52: 179–185Google Scholar
  3. Chance B, Sacktor B (1958) Respiratory metabolism of insect flight muscle. II. Kinetics of respiratory enzyme in flight muscle sarcosomes. Arch Biochem Biophys 76: 509–531PubMedCrossRefGoogle Scholar
  4. Chance B, Williams GR (1956) Adv Enzymol 17: 65–134Google Scholar
  5. Conover T, Ernster L (1960) Bypass of the amytal-sensitive site of the respiratory chain in mitochondria by means of vitamin K3. Acta Chem Scand 14: 1840–1848CrossRefGoogle Scholar
  6. Estabrock RW, Sacktor B (1958) α-glycerophosphate oxidase of flight muscle mitochondria. J Biol Chem 233:1014–1019Google Scholar
  7. Fukami J (1954) Effect of rotenone on the succinoxidase system in the muscle of the cockroach. Jpn J Appl Zool 19: 29–37Google Scholar
  8. Fukami J (1956) Effect of some insecticides on the respiration of insect organs, with special referenceto the effects of rotenone. Botyu-Kagaku 21: 122–128Google Scholar
  9. Fukami J (1961) Effect of rotenone on the respiratory enzyme system of insect muscle. Bull Natl Sci (Wellington) C 13: 33–45Google Scholar
  10. Fukami J (1976) Insecticides as inhibitors of respiration. In: Wilkinson CF (ed) Insecticide biochemistry and physiology. Plenum Press, London New York, pp. 363–396Google Scholar
  11. Fukami J (1984) Rotenone and rotenoids. Submitted to comprehensive biochemistry, physiology and pharmacology. Pergamon Press, Oxford New YorkGoogle Scholar
  12. Fukami J, Tomizawa C (1958) Effect of rotenone and its derivatives on the respiration of brain in guinea pig. Botyu-Kagaku 23: 205–208Google Scholar
  13. Fukami J, Nakatsugawa T, Narahashi T (1959) The relation between chemical structure and toxicity in rotenone derivatives. Jpn J Appl Entomol Zool 3: 259–265CrossRefGoogle Scholar
  14. Fukami J, Shishido T, Fukunaga K, Casida JE (1969) Oxidative metabolism of rotenone in mammals, fish and insects and its relation to selective toxicity. J Agric Food Chem 17: 1217–1226CrossRefGoogle Scholar
  15. Hollunger G (1955) Guanidines and oxidative phosphorylation. Acta Pharmacol Toxicol Suppl No 1 11: 84CrossRefGoogle Scholar
  16. Ilivicky J, Chefurka W, Casida JE (1967) Oxidative phosphorylation and sensitivity to uncouplers of housefly mitochondria: Influence of isolation medium. J Econ Entomol 60: 1404–1409PubMedGoogle Scholar
  17. Jeng M, Hals C, Crane FL, Takahashi S, Tamura S, Folkers K (1968) Inhibition of mitochondrial electron transport by piericidin a and related compounds. Biochemistry 7: 1311–1317PubMedCrossRefGoogle Scholar
  18. Lardy H, Ferguson SM (1969) Oxidative phosphorylation: Role of inorganic phosphate and acceptor system in control of metabolic rates. J Biol Chem 195: 215–222Google Scholar
  19. Marquardt RR, Brosemer RW (1966) Insect extramitochondrial a-glycerophosphate dehydrogenase. I. Crystallization and physical properties of the enzyme from honeybee ( Apis ellifera) thoraces. Biochim Biophys Acta 128: 454–460Google Scholar
  20. Matsuda M, Fukami J (1972) Preliminary survey of effects of phenols on the oxidative phosphorylation in the american cockroach muscle mitochondria. Appl Entomol Zool 7: 27–36Google Scholar
  21. Matsumura F, Narahashi T (1971) ATPase inhibition and electrophysiological change caused by DDT and related neuroactive agents in lobster nerve. Biochem Pharmacol 20: 825–837.PubMedCrossRefGoogle Scholar
  22. Mitsui T, Fukami J, Fukunaga K, Takahashi N, Tamura S (1969) Studies on piericidin. I. Effect of piericidin a and b on the mitochondrial electron transport in insects. Butyu-Kagaku 34: 135–139.Google Scholar
  23. Mitsui T, Fukami J, Fukunaga K, Takahashi N, Tamura S (1970) Studies on piericidin: Antagonistic effect of vitamin K3 on the respiratory chain of insects and mammals in the presence of piericidin. J Agric Biol Chem 34: 1101–1109CrossRefGoogle Scholar
  24. Nakakita H (1976) The inhibitory site of phosphine. J Pestic Sci 1: 235–238CrossRefGoogle Scholar
  25. O’Brien RD, Cheng L, Kimmel EC (1965) Inhibition of the a-glycerophosphate shuttle in housefly flight muscle. J Insect Physiol 11: 1241–1248PubMedCrossRefGoogle Scholar
  26. Price NR (1980) Some aspects of the inhibition of cytochrome c oxidase by phosphine in susceptible and resistant strains of Rhyzopertha dominica. Insect Biochem 10: 147–150CrossRefGoogle Scholar
  27. Price NR, Mills KA, Humphries LA (1982) Phosphine toxicity and catalase activity in sesceptible and resistant strains of the lesser grain borer (Phyzopertga dominica). Comp Biochem Physiol 73 C:411–413Google Scholar
  28. Sacktor B (1965) Energetics and metabolism of muscular contraction in insect mitochondria. In: Rockstein M (ed) Physiology of insecta, vol II. Academic Press, London New York, pp. 484–580Google Scholar
  29. Sacktor B (1974) Biological oxidations and energetics in insect mitochondria. In: Rockstein M (ed) Physiology of insecta, vol IV. Academic Press, London New York, pp. 271–353Google Scholar
  30. Sacktor B, Childress CC (1967) Metabolism of proline in insect flight muscles and its significance in stimulating the oxidation of pyruvate. Arch Biochem Biophys 120: 583–588CrossRefGoogle Scholar
  31. Tischler N (1936) Studies on how derris kills insects. J Econ Entomol 28: 215–219Google Scholar
  32. Tsuda S, Urakawa N, Fukami J (1977) Inhibitory effect of papaverine on a respiration-dependent contracture of guinea pig Taenia coli in high K+ medium. III. Difference effect of papaverine and rotenone on DT-diaphorase. Jpn J Pharmacol 27: 855–863PubMedCrossRefGoogle Scholar
  33. Whitehouse HW (1964) Biochem Pharmacol 13: 319PubMedCrossRefGoogle Scholar
  34. Yamaguchi I, Matsumura F, Kadous AA (1979) Inhibition of synaptic ATPase by heptachlor epoxide in rat brain. Pestic Biochem Physiol 11: 285–293CrossRefGoogle Scholar
  35. Yamaguchi I, Matsumura F, Kadous AA (1980) Heptachlor epoxide: effect on calcium mediated transmitter release from brain synaptosomes in rat. Biochem Pharmacol 29: 1815–1823PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985 1985

Authors and Affiliations

  • J. I. Fukami
    • 1
  1. 1.Laboratory of Insect ToxicologyInstitute of Physical and Chemical ResearchWako-shi, SaitamaJapan

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