Problems and Prospects of Site-Directed Transposon Mutagenesis in Azospirillum

  • Mahavir Singh
  • Walter Klingmüller
Conference paper

Summary

Different vectors and transposons were tested to obtain site-directed nif- mutants of A. brasilense. Tn5 proved more suitable than Tn1725 in this respect. A novel method of site- directed nif-gene deletion formation in combination with resistance-gene-cartridge mutagenesis is also reported. Keywords: nif-genes, transposon mutagenesis, cloning, cartridge mutagenesis, nif- mutants.

Keywords

Agar Tetracycline Acetylene Kanamycin Rifampicin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Mahavir Singh
    • 1
  • Walter Klingmüller
    • 1
  1. 1.Lehrstuhl für GenetikUniversität BayreuthBayreuthGermany

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