Problems and Prospects of Site-Directed Transposon Mutagenesis in Azospirillum

  • Mahavir Singh
  • Walter Klingmüller
Conference paper


Different vectors and transposons were tested to obtain site-directed nif- mutants of A. brasilense. Tn5 proved more suitable than Tn1725 in this respect. A novel method of site- directed nif-gene deletion formation in combination with resistance-gene-cartridge mutagenesis is also reported. Keywords: nif-genes, transposon mutagenesis, cloning, cartridge mutagenesis, nif- mutants.


Nitrogen Fixation Wild Type Strain Acetylene Reduction EcoRI Fragment Azospirillum Brasilense 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Döbereiner, J. (1984). In: Azospirillum II. Genetics, Physiology, Ecology (Ed. Klingmiiller, W.),pp. 9–23, Birkhauser, Basel.CrossRefGoogle Scholar
  2. 2.
    Hartmann, A., Singh, M. and Klingmiiller, W. (1983). Can. J. Microbiol. 29, 916–923.CrossRefGoogle Scholar
  3. 3.
    Quiviger, B., Franche, C., Lutfalla, G., Rice, D., Haselkorn, R. and Elmerich, C. (1982). Biochimie 64, 495–502.PubMedCrossRefGoogle Scholar
  4. 4.
    Jara, P., Quiviger, B. Laurent, B. and Elmerich, C. (1983). Can. J. Microbiol. 29, 968–972.CrossRefGoogle Scholar
  5. 5.
    Singh, M. and Klingmiiller, W. (1983). In: Azospirillum II. Genetics, Physiology, Ecology (Ed. Klingmiiller, W.), pp. 47– 55, Birkhauser, Basel.Google Scholar
  6. 6.
    Pedrosa, F.O. and Yates, M.G. (1984). FEMS Microbiol. Letters 23 95–101.CrossRefGoogle Scholar
  7. 7.
    Fahsold, R., Singh, M. and Klingmiiller, W. 1985 ( This volume).Google Scholar
  8. 8.
    Ruvkun, G.B. and Ausubel, F.M. (1980). Proc. Natl. Acad. Sci. USA 77, 191–195.PubMedCrossRefGoogle Scholar
  9. 9.
    Simon, R., Priefer, U. and Piihler, A. (1983). Bio/technology, 1, 784–791.CrossRefGoogle Scholar
  10. 10.
    Selvaraj, G. and Iyer, V,N. (1983). J. Bacteriol. 156, 1292–1300.PubMedGoogle Scholar
  11. 11.
    Chang, A.C.Y. and Cohen, S.N. (1978). J. Bacteriol. 134, 1141–1156.PubMedGoogle Scholar
  12. 12.
    Singh, M. and Klingmiiller, W. (1984X. In: Proceedings of the III. International Symposium on nitrogen fixation with nonlegumes, Helsinki (In press).Google Scholar
  13. 13.
    Albrecht, S.L. and Okon, Y. (1980). Methods in Enzymology 69, 740–749.CrossRefGoogle Scholar
  14. 14.
    Singh, M., Kleeberger, A. and Klingmüller, W. (1983). Mol. Gen. Genet. 190, 373–378.CrossRefGoogle Scholar
  15. 15.
    Singh, M. and Klingmiiller W. (1984). In: Trends in Molecular Genetics, U. Sinha and W. Klingmiiller (Eds.), Spectrum Publishing House, Patna, Delhi (In press).Google Scholar
  16. 16.
    Scolnik, P. and Haselkorn, R. (1984). Nature 307, 289–292.PubMedCrossRefGoogle Scholar
  17. 17.
    Perroud, B., Bhandari, S.K. and Elmerich, C. (1985). This volume.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Mahavir Singh
    • 1
  • Walter Klingmüller
    • 1
  1. 1.Lehrstuhl für GenetikUniversität BayreuthBayreuthGermany

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