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Exposure of protein antigens and reduction of siderophore production after growth of Gram-negative bacteria in Subinhibitory concentrations of cephalosporins

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The Influence of Antibiotics on the Host-Parasite Relationship II

Abstract

The β-lactams are among the most commonly used antibiotics in clinical practice. It is known that the molecular targets of β-lactam antibiotics are penicillin-binding proteins (PBPs) located in the cytoplasmic membrane of both Gram-negative and Gram-positive bacteria [24,32]. The PBPs are enzymes involved in the synthesis of peptidoglycan which plays an important role in confering rigidity and osmotic stability on the bacterial envelope [32]. Disruption of the synthesis of peptidoglycan by β-lactam antibiotics causes morphological changes and eventually lysis of the bacterial cells. Recently we found that growth of K. pneumoniae in the presence of subinhibitory concentrations of cephalosporins (not affecting growth rate) caused reduction in the production of siderophores and also in capsule formation [12]. The reduction in capsule resulted in rapid agglutination of the antibiotic treated cells of encapsulated K. pneumoniae by antisera raised against a non-encapsulated strain of K. pneumoniae[12]. This was found to be due to the exposure of outer membrane proteins antigens and possibly also lipopolysaccharide on the surface of encapsulated K. pneumoniae after growth in the presence of cephalosporins (submitted for publication).

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References

  1. Anwar H, Brown MRW, Cozens RM and Lambert PA (1983) Isolation and characterization of the outer and cytoplasmic membranes of Pseudomonas cepacia. J Gen Microbiol 129: 449–507

    Google Scholar 

  2. Anwar H, Brown MRW and Lambert PA (1983) Effect of nutrient depletion on sensitivity of Pseudomonas capacia to phagocytosis and serum bactericidal activity at different temperatures. J Gen Microbiol 129: 2021–2027

    PubMed  CAS  Google Scholar 

  3. Bjorn MJ, Sokol PA and Iglewski BH (1979) Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. J Bacteriol 138: 143–200

    Google Scholar 

  4. Braun V, Brazel-Faisst C and Schneider R (1984) Growth stimulation of Escherichia coli in serum by iron (III) aerobactin. Recycling of aerobactin. FEMS Microbios Letts 21: 99–103

    Article  CAS  Google Scholar 

  5. Brown MRW and Williams P (1985) Influence of substrate limitation and growth phase on sensitivity to antimicrobial agents. J Antimicrob Chemother 15 (Suppl A): 7–14

    PubMed  CAS  Google Scholar 

  6. Brown MRW and Williams P (1985) Influence of environment on envelope properties affecting survival of bacteria in infections. Ann Rev Microbiol 39: 527–56

    Article  CAS  Google Scholar 

  7. Crosa JH (1984) The relationship of plasmid-mediated iron transport and bacterial virulence. Ann Rev Microbiol 38: 69–89

    Article  CAS  Google Scholar 

  8. Gemell CG, Spear T and Peterson PK (1983) Morphological changes in Bacteroides fragilis and Klebsiella pneumoniae attributable to growth in the presence of various antibiotics. Eur J Clin Microbiol 2: 217–218

    Article  Google Scholar 

  9. Gibson F and Magrath DI (1969) The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobactar aerogenes. Biochim Biophys Acta 192: 175–184

    PubMed  CAS  Google Scholar 

  10. Griffiths E (1983) Availability of iron and survival of bacteria in infection. In: Easmon, CS, Jeljaszewicz J, Brown MRW and Lambert PA. Medical Microbiology, Vol. 3, Academic Press, p. 153–178

    Google Scholar 

  11. Jann K and Jann B (1983) K antigens of E. coli. In: P Kailos et al. Progress in Allergy, Vol. 33, Karger, Basel, p. 53–79

    Google Scholar 

  12. Kadurugamuwa JL, Anwar H, Brown MRW and Zak O (1985) Effect of subinhibitory concentrations of cephalosporins on surface properties and siderophore production in iron depleted Klebsiella pneumoniae. Antimicrob Agents and Chemother 27: 220–223

    CAS  Google Scholar 

  13. Klebba PA, Mcintosh MA and Neilands JB (1982) Kinetics of biosynthesis of iron regulated membrane proteins in Escherichia coli. J Bacteriol 149: 880–888

    PubMed  CAS  Google Scholar 

  14. Leong J and Neilands JB (1976) Mechanisms of siderophore iron transport in enteric bacteria. J Bacteriol 126: 823–830

    PubMed  CAS  Google Scholar 

  15. Lorian V and Atkinson B (1984) Bactericidal effect of polymorphonuclear neutrophils on antibiotic-induced filaments of Gram-negative bacilli. J Infect Dis 149: 719–727

    Article  PubMed  CAS  Google Scholar 

  16. Montgomerie JZ, Bindereif A, Neilands JB, Kalmanson GM and Guze CB (1984) Association of hydroxamate siderophore (aerobactin) with Escherichia coli: isolated from patients with bacteremia. Infect and Immun 46: 835–838

    CAS  Google Scholar 

  17. Neilands JB (1984) Siderophores of bacteria and fungi. Microbiol Sci 1: 9–14

    PubMed  CAS  Google Scholar 

  18. Ombaka EA, Cozens RM and Brown MRW (1983) Influence of nutrient limitation of growth on stability and production of virulence factors of mucoid and non-mucoid strains of Pseudomonas aeruginosa. Rev Infect Dis 5: S880–888

    Article  PubMed  Google Scholar 

  19. Payne, SM (1980) Synthesis and utilisation of siderophores by Shigella flexnevi. J Bacteriol 143: 1420–1424

    PubMed  CAS  Google Scholar 

  20. Perry RD and San Clemente CL (1979) Siderophore synthesis in Klebsiella pneumoniae and Shigella sonnei during iron deficiency. J Bacteriol 140: 1129–1132

    PubMed  CAS  Google Scholar 

  21. Poxton IR and Sutherland IW (1976) Isolation of rough mutants of Klebsiella aerogenes and their synthesis of polysaccharides. J Gen Microbiol 96: 195–202

    PubMed  CAS  Google Scholar 

  22. Rogers HJ (1973) Iron binding catechols and virulence in Escherichia coli. Infect & Immun 7: 445–456

    CAS  Google Scholar 

  23. Smith H (1977) Microbial surfaces in relation to pathogenicity. Bacteriol Rev. 41: 475–500

    PubMed  CAS  Google Scholar 

  24. Spratt BG (1975) Distinct penicillin binding proteins involved in the division, elongation and shape of Escherichia coli. Proc Natl Acad Sci USA 72: 2999–3003

    Article  PubMed  CAS  Google Scholar 

  25. Stephens DS, Krebs JW and McGee ZA (1984) Loss of pili and decreased attachment to human cells by Neisseria meningitidis and Neisseria gonorrhoea exposed to subinhibitory concentrations of antibiotics. Infect and Immun 46: 507–573

    CAS  Google Scholar 

  26. Stuart SJ, Greenwood KT and Luke RKJ (1980) Hydroxamate mediated transport of iron controlled by Col. V Plasmids. J Bacteriol 143: 35–42

    PubMed  CAS  Google Scholar 

  27. Swanson J (1981) Surface-exposed protein antigens of gonococcal outer membrane. Infect and Immun 34: 804–816

    CAS  Google Scholar 

  28. Towbin H, Stachelin T and Gordon J (1979) Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets. Procedure & some applications. Proc Natl Acad Sci USA 76: 4350–4354

    Article  PubMed  CAS  Google Scholar 

  29. Turnowsky F, Brown MRW, Anwar H and Lambert PA (1983) Effect of iron limitation of growth rate on the binding of penicillin G to penicillin binding proteins of mucoid and non-mucoid strains of Pseudomonas aeruginosa. FEMS Microbiol. Letts 17: 243–245

    Article  CAS  Google Scholar 

  30. van Oss CJ (1978) Phagocytosis as a surface phenomenon. Ann Rev Microbiol 32: 19–39

    Article  Google Scholar 

  31. van Tiel Menkveld GJ, Mentjox-Vervuurt JM, Oudega B and De Graf FK (1982) Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13. J Bacteriol 150: 490–497

    PubMed  Google Scholar 

  32. Waxman DJ and Strominger JL (1983) Penicillin binding proteins and the mechanism of action of ß-lactam antibiotics. Ann Rev Biochem 52: 825–869

    Article  PubMed  CAS  Google Scholar 

  33. Williams P, Brown MRW and Lambert PA (1984) Effect of iron deprivation on the production of siderophores and outer membrane proteins of Klebsiella aerogenes.J Gen Microbiol 130: 2357–2365

    PubMed  CAS  Google Scholar 

  34. Williams P, Lambert PA, Brown MRW and Jones JR (1984) The role of the O & K antigens in determining the resistance of Klebsiella aerogenes to serum killing and phagocytosis. J Gen Microbiol 129: 2181–2191

    Google Scholar 

  35. Williams PH (1979) Novel iron uptake system specified by Col V plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect and Immun 26: 925–932

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Microbiology Research Group, Institute of Pharmaceutical Sciences, Aston University in Birmingham, Gosta Green, Birmingham, B9 7ET, UK

    J. L. Kadurugamuwa, H. Anwar & M. R. W. Brown

  2. Pharma Research, Infectious Diseases, Ciba-Geigy Limited, CH-4402, Basel, Switzerland

    O. Zak

Authors
  1. J. L. Kadurugamuwa
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  2. H. Anwar
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  3. M. R. W. Brown
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  4. O. Zak
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Editor information

Editors and Affiliations

  1. Kinderklinik der Universität München, Lindwurmstraße 4, 8000, München 2, Germany

    Dieter Adam

  2. Institut für Medizinische Mikrobiologie, Freie Universität Berlin, Hindenburgdamm 27, 1000, Berlin 45, Germany

    Helmut Hahn

  3. Abteilung für Theoretische und Klinische Medizin, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

  4. Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

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© 1985 Springer-Verlag Berlin Heidelberg

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Kadurugamuwa, J.L., Anwar, H., Brown, M.R.W., Zak, O. (1985). Exposure of protein antigens and reduction of siderophore production after growth of Gram-negative bacteria in Subinhibitory concentrations of cephalosporins. In: Adam, D., Hahn, H., Opferkuch, W. (eds) The Influence of Antibiotics on the Host-Parasite Relationship II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70748-3_4

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  • DOI: https://doi.org/10.1007/978-3-642-70748-3_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70750-6

  • Online ISBN: 978-3-642-70748-3

  • eBook Packages: Springer Book Archive

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