Protection of tracheal explants infected with Pseudomonas aeruginosa by subinhibitory concentrations of aminoglycosides

  • N. R. Baker
  • Teresa Geers
Conference paper


Mortality rates for acute pneumonias caused by Pseudomonas aeruginosa may exceed 50% [11] and antibiotic therapy remains a difficult problem. Therapy frequently includes administration of an aminoglycoside alone or in combination with a beta-lactam antibiotic. Animal studies have shown that gentamicin or tobramycin were superior to carbenicillin or ticarcillin in controlling experimental Pseudomonas pneumonia [12]. Observations have also been made in cystic fibrosis (CF) patients with chronic P. aeruginosa lung infections which indicated that the lung functions of these patients improved following therapy with aminoglycoside antibiotics although the organisms were not cleared [9]. It seemed possible, therefore, that aminoglycoside antibiotics could prevent lung damage by a mechanism that did not involve inhibition of bacterial growth.


Cystic Fibrosis Aminoglycoside Antibiotic Tracheal Epithelium Serial Twofold Dilution Fractional Inhibitory Concentration 
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  1. 1.
    Baker NR (1982) Role of exotoxin A and proteases of Pseudomonas aeruginosa in repiratory tract infections. Can J Microbiol 28: 248–255PubMedCrossRefGoogle Scholar
  2. 2.
    Beachey EH, Eisenstein BI, Ofek I (1981) Sublethal antibiotics and bacterial adhesion. In: Adhesion and micro-organism pathogenicity (Ciba Foundation Symposium 80 ), Tunbridge Wells, London pp 288–300Google Scholar
  3. 3.
    Collier AM, Baseman JB (1973) Organ culture technique with Mycoplasmas. Ann NY Acad Sci 255: 277–289CrossRefGoogle Scholar
  4. 4.
    Dalhoff A, Doring G (1984) Effect of ciprofloxacin on the expression of exoenzymes and exotoxin of P. aeruginosa. Abstr 24th Intersci Conf Antimicro Agents Chemother abstr no 981, pg 263Google Scholar
  5. 5.
    Dalhoff A, Doring G, Goldstein W (1984) Comparative antibacterial activity of ciprofloxacin, ß-lactams and aminoglycosides against P. aeruginosa. In: Lawson D (ed) Cystic Fibrosis: Horizons Proc 9th Internat Cystic Fibrosis Congress, p 289 John Wiley & Sons, Chichester, EnglandGoogle Scholar
  6. 6.
    Glew RH, Pavuk RA (1984) Early synergistic interactions between amikacin and six ß-lactam antibiotics against multiply resistant members of the family Enterobacteriaceae. Antimicrob Agents Chemother 26: 78–381Google Scholar
  7. 7.
    Govan JRW, Fyfe JAM (1978) Mucoid Pseudomonas aeruginosa and cystic fibrosis: Resistance of the mucoid form to carbenicillin, flugloxacillin, and tobramycin and the isolation of the mucoid variants in vitro. J Antimicrob Chemother 4: 233–240PubMedCrossRefGoogle Scholar
  8. 8.
    Gray L, Kreger A (1979) Microscopic characterization of rabbit lung damage produced by Pseudomonas aeruginosa proteases. Infect Immun 23: 150–159PubMedGoogle Scholar
  9. 9.
    Hoiby N (1984) The management of Pseudomonas chest infection-the way forward. In: Lawson D (ed) Cystic Fibrosis: Horizons Proc 9th Internat Cystic Fibrosis Congress, pp 87–95 John Wiley & Sons, Chichester, EnglandGoogle Scholar
  10. 10.
    Lorian V (1980) Effects of subminimum inhibitory concentrations of antibiotics on bacteria, pp 342–408. In: Lorian V (ed) Antibiotics in laboratory medicine. The Williams & Wilkins Co, BaltimoreGoogle Scholar
  11. 11.
    Pennington JE, Reynolds HY, Carbone PP (1973) Pseudomonas pneumonia: a retrospective study of 36 cases. Am J Med 55: 155–160PubMedCrossRefGoogle Scholar
  12. 12.
    Pennington JE, Stone RM (1979) Comparison of antibiotic regimens for treatment of experimental pneumonia due to Pseudomonas. J Infect Dis 140: 881–889PubMedCrossRefGoogle Scholar
  13. 13.
    Peterson LR, Gerding DN, Moody JA, Fasching CE (1984) Comparison of azlocillin, ceftizoxime, cefoxitin, and amikacin alone and in combination against Pseudomonas aeruginosa in a neutropenic-site rabbit model. Antimicrob Agents Chemother 25: 545–552PubMedGoogle Scholar
  14. 14.
    Stephens DS, Krebs JW, McGee ZA (1984) Loss of pili and decreased attachment to human cells by Neisseria meningitidis and Neisseria gonorrhoeae exposed to subinhibitory concentrations of antibiotics. Infect Immun 46: 507–513PubMedGoogle Scholar
  15. 15.
    Warren RL, Baker NR, Johnson J, Stapleton MH (1985) Aminoglycosides selectively inhibit the accumulation of extracellular proteases of Pseudomonas aeruginosa. Antimicrob Agents Chemoth (in press April 1985)Google Scholar
  16. 16.
    Woods DE, Cryz SJ, Friedman RL, Iglewski BH (1982) Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats. Infect Immun 36: 1223–1228PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • N. R. Baker
    • 1
  • Teresa Geers
    • 1
  1. 1.Department of MicrobiologyThe Ohio State UniversityColumbusUSA

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