Cryopreservation of Plant Cell Cultures

  • U. Seitz
  • I. Reuff
  • E. Reinhard
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Plant cell cultures are potential sources for the production of specific secondary compounds. These cultures are commonly maintained by continuous subculture, which includes the disadvantages of high costs, requirement of shaking capacity, risk of contamination and, above all, the possible loss of biosynthetic capacity in long-term culture (Zenk 1978). Consequently, a culture with particular yield characteristics may not be readily available if serial passage is the only means of preservation. Among the attempts to eliminate these problems freeze preservation seems to be the most successful method in the case of cell suspensions. Until now little work has been carried out with callus cultures. The progress in this area has been reviewed recently by Withers (1980, 1982) and Henshaw et al. (1980).


Panax Ginseng Fluorescein Diacetate Freezing Resistance Biosynthetic Capacity Cryogenic Storage 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • U. Seitz
  • I. Reuff
  • E. Reinhard
    • 1
  1. 1.Pharmazeutisches Institut (Pharmazeutische Biologie)Universität TübingenTübingenGermany

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