Skip to main content

Induction of Enzymes of Phytoalexin Synthesis in Soybean Cells by Fungal Elicitor

  • Conference paper
Primary and Secondary Metabolism of Plant Cell Cultures

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Higher plants have aquired effective defence mechanisms during evolution, which secure their survival in the presence of a large variety of infective microorganisms. Resistance mechanisms of plants are expressed at different levels in host-parasite interactions including preformed physical and chemical defence barriers as well as defences triggered by the invader [16]. One type of active response of plants to attempted infection is the production of low molecular weight antimicrobial compounds called phytoalexins. Considerable evidence supports the view that the phytoalexin response is one mechanism by which plants resist diseases [6, 12, 14, 21].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Albersheim P, Valent BS (1978) Host-pathogen interactions in plants. Plants, when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics. J Cell Biol 78:627–643

    Article  PubMed  CAS  Google Scholar 

  2. Ayers AR, Ebel J, Finelli F, Berger N, Albersheim P (1976) Host-pathogen interactions. IX. Quantitative assays of elicitor activity and characterization of the elicitor present in the extracellular medium of cultures of Phytophthora megasperma var sofae. Plant Physiol 57:751–759

    Article  PubMed  CAS  Google Scholar 

  3. Ayers AR, Ebel J, Valent B, Albersheim P (1976) Host-pathogen interactions. X. Fractionation and biological activity of an elicitor isolated from the mycelial walls of Phytophthora megasperma var sofae. Plant Physiol 57:760–765

    Article  PubMed  CAS  Google Scholar 

  4. Ayers AR, Valent B, Ebel J, Albersheim P (1976) Host-pathogen interactions. XI. Composition and structure of wall-released elicitor fractions. Plant Physiol 57:766–774

    Article  PubMed  CAS  Google Scholar 

  5. Burden RS, Bailey JA (1975) Structure of the phytoalexin from soybean. Phytochemistry 14: 1389–1390

    Article  CAS  Google Scholar 

  6. Darvill AG, Albersheim P (1984) Phytoalexins and their elicitors — A defense against microbial infection in plants. Annu Rev Plant Physiol 35:243–275

    Article  CAS  Google Scholar 

  7. Dewick PM (1982) Isoflavonoids. In: Harborne JB, Mabry TJ (eds) The flavonoids: Advances in research. Chapman and Hall, London, pp 535–640

    Google Scholar 

  8. Dieter P, Marmé D (1980) Ca2+ transport in mitochondrial and microsomal fractions from higher plants. Planta 150:1–8

    Article  CAS  Google Scholar 

  9. -Ebel J (1984) Induction of phytoalexin synthesis in plants following microbial infection or treatment with elicitors. In: Ory RL, Rittig FR (eds) Bioregulators, chemistry and uses, ACS Symp Ser, vol 257. Am Chem Soc, Washington DC, pp 257–271

    Chapter  Google Scholar 

  10. Ebel J, Ayers AR, Albersheim P (1976) Host-pathogen interactions. XII. Response of suspension-cultured soybean cells to the elicitor isolated from Phytophthora megasperma var sofae, a fungal pathogen of soybeans. Plant Physiol 57:775–779

    Article  PubMed  CAS  Google Scholar 

  11. Ebel J, Schmidt WE, Loyal R (1984) Phytoalexin synthesis in soybean cells: Elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs and correlation with phytoalexin accumulation. Arch Biochem Biophys 232:240–248

    Article  PubMed  CAS  Google Scholar 

  12. Grisebach H, Ebel J (1978) Phytoalexins, chemical defense substances of higher plants? Angew Chem 17:635–657 (Int Ed Engl)

    Google Scholar 

  13. Hahn MG, Grisebach H (1983) Cyclic AMP is not involved as a second messenger in the response of soybean to infection by Phytophthora megasperma f sp glycinea. Z Naturforsch 38c: 578–582

    CAS  Google Scholar 

  14. Hahn MG, Bonhoff A, Grisebach H (1985) Quantitative localization of the phytoalexin glyce-ollin I in relation to fungal hyphae in soybean roots infected with Photophthora megasperma f sp glycinea. Plant Physiol 77:591–601

    Article  PubMed  CAS  Google Scholar 

  15. Hille A, Purwin C, Ebel J (1982) Induction of enzymes of phytoalexin synthesis in cultured soybean cells by an elicitor from Phytophthora megasperma f sp glycinea. Plant Cell Rep 1: 123–127

    Article  CAS  Google Scholar 

  16. Horsfall JC, Cowling EB (eds) (1980) Plant disease, vol V. Academic Press, London New York

    Google Scholar 

  17. Kreuzaler F, Ragg H, Fautz E, Kuhn DN, Hahlbrock K (1983) UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc Natl Acad Sci USA 80:2591–2593

    Article  PubMed  CAS  Google Scholar 

  18. Leube J, Grisebach H (1983) Further studies on induction of enzymes of phytoalexin synthesis in soybean and cultured soybean cells. Z Naturforsch 38c:730–735

    CAS  Google Scholar 

  19. Lyne RL, Mulheim LJ (1978) Minor pterocarpinoids of soybean. Tetrahedron Lett 3127–3128

    Google Scholar 

  20. Lyne RL, Mulheim LJ, Leworthy DP (1976) New pterocarpinoid phytoalexins of soybean. J Chem Soc Chem Commun:497–498

    Google Scholar 

  21. Mansfield JW (1982) The role of phytoalexins in disease resistance. In: Bailey JA, Mansfield JW (eds) Phytoalexins. Blackie, Glasgow, pp 253–288

    Google Scholar 

  22. Moesta P, Grisebach H (1981) Investigation of the mechanism of phytoalexin accumulation in soybean induced by glucan or mercuric chloride. Arch Biochem Biophys 211:39–41

    Article  PubMed  CAS  Google Scholar 

  23. Moesta P, Grisebach H (1981) Investigation of the mechanism of glyceollin accumulation in soybean infected by Phytophthora megasperma f sp glycinea. Arch Biochem Biophys 212: 462–467

    Article  PubMed  CAS  Google Scholar 

  24. Schmelzer E, Börner H, Grisebach H, Ebel J, Hahlbrock K (1984) Phytoalexin synthesis in soybean (Glycine max). Similar time courses of mRNA induction in hypocotyls infected with a fungal pathogen and in cell cultures treated with fungal elicitor. FEBS Lett 172:59–63

    Article  CAS  Google Scholar 

  25. Sharp JK, McNeil M, Albersheim P (1984) The primary structures of one elicitor-active and seven elicitor-inactive hexa-β-D-glucopyranosyl-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f sp glycinea. J Biol Chem 259:11321–11336

    PubMed  CAS  Google Scholar 

  26. Zähringer U, Ebel J, Mulheim LJ, Lyne RL, Grisebach H (1979) Induction of phytoalexin synthesis in soybean. Dimethylallylpyrophosphate:trihydroxypterocarpan dimethylallyl transferase from elicitor-induced cotyledons. FEBS Lett 101:90–92

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Ebel, J., Stäb, M.R., Schmidt, W.E. (1985). Induction of Enzymes of Phytoalexin Synthesis in Soybean Cells by Fungal Elicitor. In: Neumann, KH., Barz, W., Reinhard, E. (eds) Primary and Secondary Metabolism of Plant Cell Cultures. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70717-9_24

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70717-9_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70719-3

  • Online ISBN: 978-3-642-70717-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics