Skip to main content
SpringerLink
Log in

Plant Cell and Tissue Culture of Cinchona Species

  • Conference paper
Primary and Secondary Metabolism of Plant Cell Cultures

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

The genus Cinchona, belonging to the family Rubiaceae, is still esteemed for its anti-malaria activity. Several species, e.g. C. pubescens (syn. C succirubra) and C. ledgeriana,, have been cultured in plantations during the past 130 years for the production of Cinchona bark, the raw material for the alkaloids quinine and quinidine. Cinchona plantations are mainly found in Middle-America, Central Africa, India, Phillipines, and Indonesia. After 7–12 years of growth the bark of the trees is harvested. The alkaloid content is then about 10%–15% (Smit 1984).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anderson LA, Keene AT, Phillipson JD (1982) Alkaloid production by leaf organ, root organ and cell suspension cultures of Cinchona ledgeriana. Planta Med 46:25–27

    Article  PubMed  CAS  Google Scholar 

  • Chatterjee SK (1974) Vegetative propagation of high quinine yielding Cinchona. Indian J Hortic 31:174–177

    Google Scholar 

  • Fossard RA de, Myint A, Lee ECM (1974) A broad spectrum tissue culture experiment with tobacco (Nicotiana tabacum) pith tissue culture. Physiol Plant 30:125–130

    Article  Google Scholar 

  • Harkes PAA, Kreijbolder L, Libbenga KR, Wijnsma R, Nsengiyaremge T, Verpoorte R (1984) Influence of various media constituents on the growth of Cinchona ledgeriana tissue cultures and the production of alkaloids and anthraquinones therein. (Submitted)

    Google Scholar 

  • Hunter CS (1979) In vitro culture of Cinchona ledgeriana L. J Hortic Sci 54:111–114

    Google Scholar 

  • Hunter CS, McCalley DV, Barraclough AJ (1982) Alkaloids produced by cultures of Cinchona ledgeriana L. In: Fujiwara A (ed) Proc 5th Int Congr Plant Tissue Cell Cult, Plant Tissue Culture. Tokyo, pp 317–318

    Google Scholar 

  • Keene AT, Anderson LA, Phillipson JD (1983) Investigation of Cinchona leaf alakloids by High-performance liquid chromatography. J Chromatogr 260:123–128

    Article  CAS  Google Scholar 

  • Klein Horsman-Relijk J (1960) Onderzoek naar de biosynthese van enkele alkaloiden in Cinchona succirubra. Thesis, Amsterdam

    Google Scholar 

  • Koblitz H, Koblitz D, Schmauder HP, Gröger D (1983a) Studies on tissue cultures of the genus Cinchona L. Plant Cell Rep 2:95–97

    Article  Google Scholar 

  • Koblitz H, Koblitz D, Schmauder HP, Gröger D (1983b) Studies on tissue cultures of the genus Cinchona L. Alkaloid production in cell suspension cultures. Plant Cell Rep 2:122–125

    Article  CAS  Google Scholar 

  • Krikorian AD, Singh M, Quinn CE (1981) Aseptic micropropagation of Cinchona: prospects and problems. In: Rao AN (ed) Proceedings of the COSTED Symposium on tissue culture of economically important plants. Singapore, pp 167–174

    Google Scholar 

  • McCalley DV (1983) Analysis of Cinchona alkaloids by high-performance liquid chromatography. J Chromatogr 260:184–188

    Article  CAS  Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, Baerheim Svendsen A (1982a) Tissue culture of Cinchona pubescens: Effects of media modifications on the powth. Planta Med 44:237–240

    Article  PubMed  CAS  Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, Graaf YP de, Kreek M van der, Baerheim Svendsen A (1982b) The effects of plant growth regulators and culture conditions on the growth and the alkaloid content of callus cultures of Cinchona pubescens. Planta Med 46:15–18

    Article  PubMed  CAS  Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, Water A de, Gessel M van, Oeveren BCJA van, Baerheim Svendsen A (1982c) Identification of the alkaloids and anthraquinones in Cinchona ledgeriana callus cultures. Planta Med 46:19–24

    Article  PubMed  CAS  Google Scholar 

  • Mulder-Krieger Th, Verpoorte R, Kreek M van der, Baerheim Svendsen A (1984) Identification of alkaloids and anthraquinones in Cinchona pubescens callus cultures; the effect of plant growth regulators and light on the alkaloid content. Planta Med 50–1:17–23

    Article  Google Scholar 

  • Neumann D, Krauss G, Hieke M, Gröger D (1983) Indole alkaloid formation and storage in cell suspension cultures of Catharanthus roseus. Planta Med 48:20–23

    Article  PubMed  CAS  Google Scholar 

  • Noerhadi E (1981) Plant tissue culture research in Indonesia. In: Rao AN (ed) Proceedings of the COSTED Symposium on tissue culture of economically important plants. Singapore, pp 290–292

    Google Scholar 

  • Robins RJ,Webb AJ, Rhodes MJC, Payne J, Morgan MRA (1984) Radioimmunoassay for the quantitative determination of quinine in cultured plant tissues. Planta Med 50:235–238

    Article  PubMed  CAS  Google Scholar 

  • Schuite U, El-Shagi H, Zenk MH (1984) Optimization of 19 rubiaceae species in cell culture for the production of anthraquinones. Plant Cell Rep 3:51–54

    Article  Google Scholar 

  • Smit EHD (1984) Verleden, heden en perspectieven van Cinchona spp. Pharm Weekbl 119:159–162

    Google Scholar 

  • Staba EJ, Chung AC (1981) Quinine and quinidine production by Cinchona leaf, root, and unorganized cultures. Phytochemistry 20:2495–2498

    Article  CAS  Google Scholar 

  • Verpoorte R, Baerheim Svendsen A (1984) J Chromatogr Library 23B:Chromatography of alkaloids — GC and HPLC

    Google Scholar 

  • Verpoorte R, Mulder-Krieger Th, Troost JJ, Baerheim Svendsen A (1980) Thin-layer chromatographic separation of Cinchona alkaloids. J Chromatogr 184:79–96

    Article  CAS  Google Scholar 

  • Verpoorte R, Mulder-Krieger Th, Verzijl MJ, Verzijl JM, Baerheim Svendsen A (1983) High-performance liquid chromatography of Cinchona alkaloids. I. Normal-phase HPLC. J Chromatogr 261:172–175

    Article  CAS  Google Scholar 

  • Verpoorte R, Mulder-Krieger Th, Wijnsma R, Verzijl JM, Baerheim Svendsen A (1984) HPLC analysis of alkaloids in extracts of callus cultures of Cinchona spp. Z Naturforsch 39c:680–682

    CAS  Google Scholar 

  • Warhurst DC (1981) Cinchona alkaloids and malaria. The Lancet: 1346

    Google Scholar 

  • Weiler E (1982) Application of immunoassay techniques in pharmacognosy. Pharm Weekbl 117: 477–479

    CAS  Google Scholar 

  • Whitten GH, Dougall DK (1981) Quinine and quinidine accumulation by root, callus and suspension cultures of Cinchona ledgeriana. In vitro 17:220

    Google Scholar 

  • Wynsma R, Verpoorte R, Mulder-Krieger Th, Baerheim Svendsen A (1984) Anthraquinones in callus cultures of Cinchona ledgeriana. Phytochemistry 23:2307–2311

    Article  Google Scholar 

  • Zèches M, Richard B, Thepenier P, Le Men-Olivier L, Le Men J (1980) Alcaloides des feuilles du Cinchona ledgeriana. Phytochemistry 19:2451–2454

    Article  Google Scholar 

  • Zenk MH, El-Shagi H, Schulte U (1975) Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Med Suppl:78–101

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center of Bio-Pharmaceutical Sciences, Division of Pharmacognosy, Biotechnology Delft Leiden (BDL), State University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    R. Verpoorte, R. Wijnsma, Th. Mulder-Krieger & A. Baerheim Svendsen

  2. Department of Plant Molecular Biology, Biotechnology Delft Leiden (BDL), State University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    P. A. A. Harkes

Authors
  1. R. Verpoorte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Wijnsma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Th. Mulder-Krieger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. A. A. Harkes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Baerheim Svendsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Pflanzenernährung, Abteilung Gewebekultur, Justus-Liebig-Universität, Südanlage 6, 6300, Gießen, Germany

    Karl-Hermann Neumann

  2. Lehrstuhl für Biochemie der Pflanzen, Westfälische Wilhelms-Universität, 4400, Münster, Germany

    Wolfgang Barz

  3. Institut für Pharmazie, Universität Tübingen, Auf der Morgenstelle, 7400, Tübingen, Germany

    Ernst Reinhard

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Verpoorte, R., Wijnsma, R., Mulder-Krieger, T., Harkes, P.A.A., Baerheim Svendsen, A. (1985). Plant Cell and Tissue Culture of Cinchona Species. In: Neumann, KH., Barz, W., Reinhard, E. (eds) Primary and Secondary Metabolism of Plant Cell Cultures. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70717-9_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70717-9_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70719-3

  • Online ISBN: 978-3-642-70717-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation