Histologie Study of Tritiated Thymidine Incorporation by Trophoblastic Villi in the First Trimester

  • L. Koulischer
  • J. Hustin
  • Y. Gillerot


With the recently introduced chorionic villi sampling techniques, the study of fetal chromosomes during the first trimester of pregnancy has become possible. After the biopsy has been performed, the length of time necessary to obtain suitable mitoses for karyotype analysis varies from a few hours with the direct technique (Simoni et al. 1983) to 10–21 days (mean, 16 days) with tissue cultures of macerated villi (Heaton et al. 1984). From the histologic point of view, a single villus of the first trimester shows a mesenchymal core, which is surrounded by a inner layer of cytotrophoblast and an outer layer of syncytiotrophoblast and ends with a dense cytotrophoblastic cell column (Boyd and Hamilton 1970). Dividing cells are observed in the mesenchymal core and the cytotrophoblast; to the contrary, the syncytiotrophoblast shows no mitoses. Shortly after biopsy, DNA synthesis, as demonstrated by tritiated thymidine incorporation, occurs in the cytotrophoblast and the villous core (Gerbie et al. 1968); this activity persists several days in vitro.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Boyd JD, Hamilton WJ (1970) The human placenta, vol I. Heffer, CambridgeGoogle Scholar
  2. 2.
    Gerbie AB, Hathaway HH, Brewer JI (1968) Autoradiographic, analysis of normal trophoblastic proliferation. Am J Obstet Gynecol 100: 640–645PubMedGoogle Scholar
  3. 3.
    Gustavii B (1984) Chorionic villi sampling under direct vision. Clin Genet 26: 297–300PubMedCrossRefGoogle Scholar
  4. 4.
    Heaton DR, Czepulkowski BH, Horwell DH, Coleman DV (1984) Chromosome analysis of first trimester chorionic villus biopsies prepared by a maceration technique. Prenat Diagn 4: 279–288PubMedCrossRefGoogle Scholar
  5. 5.
    Hustin J, Foidart JM, Lambotte R (1984) Cellular proliferation in villi of normal and pathological pregnancies. Gynecol Obstet Invest 17: 1–9PubMedCrossRefGoogle Scholar
  6. 6.
    Niazi M, Coleman DV, Coeffler FE (1981) Trophoblast sampling in early pregnancy. Culture of rapidly dividing cells from immature placental villi. Br J Obstet Gynecol 88: 1081–1085CrossRefGoogle Scholar
  7. 7.
    Simoni G, Brambati B, Danesino C, Rossella F, Terzoli GL, Ferrari M, Fraccaro M (1983) Efficient direct chromosome analysis and enzyme determination from chorionic villi samples in the first trimester of pregnancy. Hum Genet 63: 349–357PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • L. Koulischer
    • 1
  • J. Hustin
    • 1
    • 2
  • Y. Gillerot
    • 1
  1. 1.Institute of Pathologic MorphologyGerpinnes (Loverval)Belgium
  2. 2.Department of Gynecology-ObstetricsUniversity of LiègeBelgium

Personalised recommendations