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Molecular Cloning of a Gene Sequence Induced by NGF in PC12 Cells

  • A. Levi
  • J. D. Eldridge
  • B. M. Paterson
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Using a differential hybridization technique to screen a cDNA library constructed with mRNA from PC 12 (Green and Tischler 1976) cells treated with nerve growth factor (NGF) (Levi Montalcini 1966), we have characterized a cDNA clone, VGF8a, corresponding to a mRNA induced to high levels as an early response to NGF. Preliminary experiments suggest that the expression of such a mRNA is tissue specific and is restricted to nerve cells.

Keywords

PC12 Cell Nerve Growth Factor cDNA Library cDNA Clone Molecular Cloning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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  4. Green LA, Tischler AS (1976) Natl Acad Sci USA 73: 2424CrossRefGoogle Scholar
  5. Levi Montalcini R (1966) Harvey Lect 60: 21Google Scholar
  6. McGuire JC, Greene LA (1978) Cell 15: 357PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • A. Levi
  • J. D. Eldridge
  • B. M. Paterson
    • 1
  1. 1.Laboratory of Biochemistry, National Cancer InstituteNational Institutes of HealthBethesdaUSA

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