Molecular Cloning of a Gene Sequence Induced by NGF in PC12 Cells

  • A. Levi
  • J. D. Eldridge
  • B. M. Paterson
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Using a differential hybridization technique to screen a cDNA library constructed with mRNA from PC 12 (Green and Tischler 1976) cells treated with nerve growth factor (NGF) (Levi Montalcini 1966), we have characterized a cDNA clone, VGF8a, corresponding to a mRNA induced to high levels as an early response to NGF. Preliminary experiments suggest that the expression of such a mRNA is tissue specific and is restricted to nerve cells.


PC12 Cell Nerve Growth Factor cDNA Library cDNA Clone Molecular Cloning 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • A. Levi
  • J. D. Eldridge
  • B. M. Paterson
    • 1
  1. 1.Laboratory of Biochemistry, National Cancer InstituteNational Institutes of HealthBethesdaUSA

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