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Study of Ecto-5′-Nucleotidase, Ecto-ATPase, and Adenosine Deaminase Activities in Relation to Lymphoid Cell Differentiation

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Cellular Biology of Ectoenzymes

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Normal lymphocytes have very low levels of de novo purine biosynthesis and are highly dependent on the purine salvage pathway. The discovery that several immunodeficiencies are linked to deficiencies in some of the enzymes of this metabolic pathway (Giblett et al. 1972) have focused attention on the previously unknown relationship between nucleotide metabolism and lymphocyte differentiation and function.

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References

  • Astaldi AA, Leupers CJM, Schellekens PTA (1981) Adenosine induces immunological mat-uration of thymocytes. Immunobiology. 159: 93

    Article  Google Scholar 

  • Barton RW, Goldschneider I (1978) 5′nucleotidase activity on subpopulations of rat lymphocytes. J Immunol 121: 2329–2334

    PubMed  CAS  Google Scholar 

  • Barton RW, Martiniuk F, Hirschorn R, Goldschneider I (1979) The distribution of adenosine deaminase among lymphocyte populations in the rat. J Immunol 122: 216–220

    PubMed  CAS  Google Scholar 

  • Bastian JF, Ruedi JM, McPherson GA, Colembesky HE, O’Connor RD, Thompson LF (1984) Lymphocyte ecto 5′nucleotidase in infancy: increasing activity in peripheral blood B cells precedes their ability to synthesize IgG in vitro. J Immunol 132: 1767–1772

    PubMed  CAS  Google Scholar 

  • Bonnafous JC, Dornand J, Mani JC (1979a) Adenosine induced cAMP increase in pig lymphocytes is not related to adenylate cyclase stimulation. Biochim Biophys Acta 587: 180–191

    Article  PubMed  CAS  Google Scholar 

  • Bonnafous JG, Dornand J, Mani JC (1979b) Hormone like action of adenosine in mouse thymocytes and splenocytes. Evidence for the existence of membrane adenosine coupled to adenylate cyclase. FEBS Lett 107: 95–99

    Article  PubMed  CAS  Google Scholar 

  • Bonnafous JC, Dornand J, Favero J, Mani JC (1982) Lymphocyte membrane adenosine receptors coupled to adenylate cyclase. Properties and occurence in various lymphocyte subclasses. J Recept Res 2: 347–366

    CAS  Google Scholar 

  • Bonnafous JC, Dornand J, Favero J, Mani JC (1983) Role of adenosine in lymphocyte mat-uration: possible involvement of adenosine receptors coupled to adenylate cyclase. In: Parker JW, O’Brien RL (eds) Intercellular communication in leucocyte function. Wiley, New York, pp 437–440

    Google Scholar 

  • Boss GB, Thompson LF, Spieglberg HL, Pichler WJ, Seegmiller JE (1980) Age dependency of lymphocyte 5 Nucleotidase. J Immunol 125: 679–682

    PubMed  CAS  Google Scholar 

  • Carraway C, Carraway KL (1976) ConcanavalinA perturbation of membrane enzymes of mammary gland. J Supramol Struct 4: 121–126

    Article  PubMed  CAS  Google Scholar 

  • Chen SS, Tung JJ, Gillis S, Good RA, Hadden JW (1983) Changes in surface antigens of im-mature thymocytes under the influence of T cell growth factor and thymic factors. Proc Natl Acad Sci USA 80: 5980–5984

    CAS  Google Scholar 

  • Chiba P, Kraupp M, Rumpold H, Muller MM (1984) Uptake of AMP into K562 and HL-60 leukemic cell lines. In: DeBruyn CHM, Simmonds HA, Muller MM (eds) Advances in experimental medicine and biology, vol 165B. Plenum, New York, pp 273–278

    Google Scholar 

  • Cohen A, Dosh HM, Gelfand EW (1981) Induction of ecto 5 Nucleotidase activity in human thymocytes. Clin Immunol Immunopathol 18: 287–290

    Article  PubMed  CAS  Google Scholar 

  • De Pierre JW, Karnovsky ML (1974) Ectoenzymes of the guinea pig polynuclear leukocyte I. Evidence for an ecto adenosine monophosphatase, adenosine triphosphatase, and p- nitrophenylphosphatase. J Biol Chem 249: 7111–7120

    Google Scholar 

  • Dornand J, Mani JC, Mousseron-Canet M, Pau B (1974) Propriétés d’une ATPase Ca+ ou Mg2+ dépendante des membranes plasmiques de lymphocytes. Effet de la concanavalineA surles ATPases membranaires. Biochimiè(Paris) 56: 851–857

    Google Scholar 

  • Dornand J, Reminiac C, Mani JC (1977) Activité 5′AMPase des membranes plasmiques de lymphocytes. Effet de la concanavalineA. Biochimie (Paris) 59: 425–432

    CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Mani JC (1978a) Purification and properties of 5′nucleotidase from lymphocyte plasma membranes. Eur J Biochem 87: 459–465

    Article  PubMed  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Mani JC (1978b) Effects of ConA and other lectins on pure 5′nucleto- tidase isolated from lymphocyte plasma membranes. Biochem Biophys Res Commun 82: 685–692

    Article  PubMed  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Gavach C, Mani JC (1979) 5′Nucleotidase-facilitated transport by mouse lymphocytes. Biochimie (Paris) 61: 973–978

    CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Mani JC (1980a) 5′Nucleotidase of two populations of mouse thymocytes separated by PNA agglutination. FEBS Lett 118: 225–228

    Article  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Mani JC (1980b) 5′Nucleotidase-adenylate cyclase relationships in mouse thymocytes. A réévaluation of the effects of concanavalinA on cyclic AMP levels. FEBS Lett 110: 30–34

    Article  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Favero J, Mani JC (1982a) Ecto-5 Nucleotidase and adenosine de-aminase activities of lymphoid cells. Biochem Med 28: 144–156

    Article  PubMed  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Favero J, Mani JC (1982b) Purine production in mature and immature human lymphocytes. FEBS Lett 142: 88–92

    Article  PubMed  CAS  Google Scholar 

  • Dornand J, Bonnafous JC, Favero J, Gartner A, Dauthuille I, Poncelet P, Caxayon P, Pau B, Mani JC (1984a) An immunofluorescent technic for the determination of cell surface 5Nucleotidase. In: Rosenfeld C, Serrou B, Vaillet P (eds) Fluorescent technic and membrane markers in cancer and immunology: membrane dynamics, cellular characterization and cell sorting pp 349–354

    Google Scholar 

  • Dornand J, Bonnafous JC, Favero J, Gartner A, Mani JC (1984b) The proposed 5′nucleotidase inhibitor in human cells is an artefact. Biochim Biophys Acta 804: 398–403

    Article  PubMed  CAS  Google Scholar 

  • Edwards NL, Magilavy DB, Cassidy JT, Fox IH (1978) Lymphocyte ecto-5′Nucleotidase deficiency in agammaglobulinemia. Science (Wash DC) 201: 628–630

    Article  CAS  Google Scholar 

  • Ganeshagunu K, Lee N, Llewellin P, Prentice HG, Hoffrand AV, Catovsky D, Habeshaw JA, Robinson J, Greaves MF (1981) Adenosine deaminase concentrations in leukemia and lymphoma: relation to cell phenotypes. Leuk Res 5: 215–222

    Article  Google Scholar 

  • Giblett ER, Anderson JE, Cohen F, Pollara B, Meurvissen HJ (1972) Adenosine deaminase deficiency in two patients with severely impaired cellular immunity. Lancet 2: 1067–1069

    Article  PubMed  CAS  Google Scholar 

  • Gregory SH, Kern M (1979) The heterogeneous distribution of 5′nucleotidase among rabbit lymphocytes. J Immunol 123: 1978–1982

    Google Scholar 

  • Gutensohn W, Gurtler LG, Siegert W, Eichler E, Ernst S (1980) Ectoenzymes on the surface of cells from human lymphoblastoid lines: 5′-nucleotidase and phosphatase. Blut 41: 411–420

    Article  PubMed  CAS  Google Scholar 

  • Gutensohn W, Thiel E (1981) High levels of 5′-nucleotidase activity in blastic chronic mye-logenous leukemia with common ALL-antigen. Leuk Res 5: 505–510

    Article  PubMed  CAS  Google Scholar 

  • Gutensohn W, Wunsch B, Rhodt H (1983) Independent expression of the surface markers 5′nucleotidase and CALLA on leukemic cells. Blut 46: 119–207

    Article  Google Scholar 

  • Johnson SM, Noerth MS, Asherson GL, Allsop J, Watts RWE, Webster ADB (1977) Lymphocyte purine 5′nucleotidase deficiency in primary hypogammaglobulinemia. Lancet i: 168–170

    Article  Google Scholar 

  • Koya M, Kanoth T, Sawada H, Uchina H, Ueda K (1981) Adenosine deaminase and ecto 5′ nucleotidase activities in various leukemias with special reference to blast crisis of chronic myeloid leukemia. Blood 58: 1107–1111

    PubMed  CAS  Google Scholar 

  • Pazmino N, McEwan R, Ihle J (1977) Distribution of terminal deoxynucleotidyl transferase in bovine serum albumin gradient fractionated thymocytes and bone marrow cells of normal and leukemic mice. J Immunol 119: 494–499

    PubMed  CAS  Google Scholar 

  • Petitou M, Tuy F, Rosenfeld C, Mishal Z, Paintrand M, Jasmin C, Mathe G, Inbar M (1978) Decreased microviscosity of membrane lipid in leukemic cells: two possible mechanism. Proc Natl Acad Sci USA 75: 2306–2310

    Article  PubMed  CAS  Google Scholar 

  • Phuc LH, Papiernik M, Berrih S, Duval D (1981) Thymic involution in pregnant mice. Clin Exp Immunol 44: 247–252

    PubMed  CAS  Google Scholar 

  • Quagliata F, Conklyn M, Sibler R (1974) Studies on the lymphocyte 5′nucleotidase in chronic lymphocyte leukemia, infectious mononucleosis, normal subpopulations, and phytohemag- glutinin stimulated cells. Cancer Res 34: 3197–3202

    PubMed  CAS  Google Scholar 

  • Reaman GH, Blatt J, Poplack DG (1981) Lymphoblast purine pathway enzymes in B cell acute lymphoblastic leukemia. Blood 58: 330–332

    PubMed  CAS  Google Scholar 

  • Reisner Y, Linker Israeli M, Sharon N (1976) Separation of mouse thymocytes into the two subpopulations by the use of the peanut agglutinin. Cell Immunol 25: 129–134

    Article  PubMed  CAS  Google Scholar 

  • Roelants GE, London J, Major-Withey KS, Serrano B (1979) Peanut agglutinin II- Characterization of the Thy-I, Tla and Ig phenotypes of peanut agglutinin positive cells in adult, embryonic and nude mice using double immunofluorescence. Eur J Immunol 9: 139–145

    Article  PubMed  CAS  Google Scholar 

  • Rowe M, De Gast CG, Platts-Mills TAE, Asherson GL, Webster ADB, Johnson SM (1979) 5′ nucleotidase of B and T lymphocytes isolated from human peripheral blood. Clin Exp Immunol 36: 97–101

    PubMed  CAS  Google Scholar 

  • Rowe M, De Gast CG, Platts-Mills TAE, Asherson GL, Webster ADB, Johnson SM (1980) Lym-phocyte 5′nucleotidase deficiency in primary hypogammaglobulinemia and cord blood. Clin Exp Immunol 39: 337–343

    PubMed  CAS  Google Scholar 

  • Smith GP, Shah T, Webster ADB, Peters TJ (1981) Studies on the kinetic properties and subcellular localization of adenosine diphosphatase activity in human peripheral blood lymphocytes. Clin Exp Immunol 46: 321–326

    PubMed  CAS  Google Scholar 

  • Sun AS, Holland JF, Ohnuma T, Slankard-Chahinian (1982) 5′nucleotidase activity in permanent human lymphoid cell lines. Biochim Biophys Acta 714: 530–535

    Article  PubMed  CAS  Google Scholar 

  • Sun AS, Holland JF, Lin K, Ohnuma T (1983) Implications of a 5′nucleotidase inhibitor in human leukemic cells for cellular aging and cancer. Biochim Biophys Acata 762: 577–584

    Article  CAS  Google Scholar 

  • Thompson LF, Boss GR, Spiegelberg HL, Jansen IV, O’Connor RD, Waldmann TA, Hamburger RN, Seegmiller JE (1979) Ecto-5′nucleotidase activity in T and B lymphocytes from normal subjects and patients with congenital X-linked agammaglobulinemia. J Immunol 123: 2475–2478

    PubMed  CAS  Google Scholar 

  • Uusitalo RJ, Karnovsky MJ (1977) Surface localization of 5′nucleotidase on the mouse lympho-cytes. J Histochem Cytochem 25: 87–96

    Article  PubMed  CAS  Google Scholar 

  • Webster ADB, Shah T, Peters TJ (1984) Lymphocyte ecto-5′nucleotidase in immunodeficiency and leukemia. In: De Bruyn CHMM, Simmonds HA, Muller MM (eds) Advances in experimental medicine and biology, vol 165 A. Plenum, New York, pp 67–72

    Google Scholar 

  • Wortmann RL, Mitchell BS, Edwards NL, Fox IM (1979) Biochemical basis for differential deoxyadenosine toxicity to T and B lymphoblasts: Role for 5′nucleotidase. Proc Natl Acad Sci USA 76: 2434–2437

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratoire de Biochimie des Membranes, E.R.CNRS 228, E.N.S.C.M., 8, Rue de l’Ecole Normale, 34075, Montpellier Cedex, France

    J. C. Mani

Authors
  1. J. Dornand
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  2. J. C. Bonnafous
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  3. A. Gartner
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  4. J. Favero
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  5. J. C. Mani
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Editor information

Editors and Affiliations

  1. Abt. Neuromorphologie, Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18a, 8033, Martinsried, Germany

    Georg W. Kreutzberg  & Martin Reddington  & 

  2. AK Neurochemie, Zoologisches Institut der Universität, Siesmayerstraße 70, 6000, Frankfurt/M., Germany

    Herbert Zimmermann

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© 1986 Springer-Verlag Berlin Heidelberg

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Dornand, J., Bonnafous, J.C., Gartner, A., Favero, J., Mani, J.C. (1986). Study of Ecto-5′-Nucleotidase, Ecto-ATPase, and Adenosine Deaminase Activities in Relation to Lymphoid Cell Differentiation. In: Kreutzberg, G.W., Reddington, M., Zimmermann, H. (eds) Cellular Biology of Ectoenzymes. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70664-6_7

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  • DOI: https://doi.org/10.1007/978-3-642-70664-6_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70666-0

  • Online ISBN: 978-3-642-70664-6

  • eBook Packages: Springer Book Archive

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