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Lactate:Glycolytic End Product and Oxidative Substrate During Sustained Exercise in Mammals — The “Lactate Shuttle”

  • G. A. Brooks
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Results of kinetic tracer studies on several mammalian species during rest and prolonged sustained exercise indicate that lactate is a metabolic intermediate which is very active in supplying carbon for a number of important physiological processes. Lactate is a product of glycolysis and glycogenolysis, and it is a precursor for glucose and glycogen resynthesis. Additionally, lactate produced at some sites during exercise is also a substrate for oxidative energy transduction at other sites. This “shuttling of lactate” through the interstitium and vasculature can be quantitatively as important as the release of glucose from the liver for supplying oxidizable substrate. Because much of the glycolytic and gluconeogenic flux passes through the lactate pool, the metabolism of lactate emerges as a critically important component in the overall integration and regulation of intermediary metabolism. This dynamic role of lactate in mammals, as described here, is different from the stagnant role of lactate portrayed from measurements of lactate concentration in the blood and muscle of mammals and other species. Rather than a dead-end metabolite which accumulates as the resuit of muscle anoxia during exercise and waits until the recovery (“O2 debt”) period to be returned to glucose and glycogen, lactate is a dynamic metabolite which turns over rapidly and which can participate in a number of processes during rest, exercise, and recovery from exercise.

Keywords

Blood Lactate Lactate Production Lactate Accumulation Blood Lactate Level Dynamic Steady State 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bang O (1936) The lactate content of the blood during and after muscular exercise in man. Scand Arch Physiol 74 (Suppl 10): 49–82Google Scholar
  2. Brooks GA (1985) Anaerobic threshold: Review of the concept and directions for future research. Med Sci Sports Exercise (in press)Google Scholar
  3. Brooks GA, Donovan CM (1983) Effect of endurance training on glucose kinetics during exercise. Am J Physiol 244 (Endocrinol Metab 7): E505–E512PubMedGoogle Scholar
  4. Brooks GA, Fahey TD (1984) Exercise physiology: Human biogenergetics and its applications.Google Scholar
  5. John Wiley, New York, p 79Google Scholar
  6. Brooks GA, Mazzeo RS, Schoeller DA, Budinger TF (1984) Lactate turnover in man during rest and greated exercise. Med Sci Sports Exercise 16: 121Google Scholar
  7. Chance B, Quinstorff B (1978) Study of tissue oxygen gradients by single and multiple indicators. Adv Exp Med Biol 94: 331–338Google Scholar
  8. Connett RJ, Gaueski TEJ, Honig CR (1984) Lactate accumulation in fully aerobic, working dog gracilis muscle. Am J Physiol 246 (Heart Cire Physiol 15): H120–H128PubMedGoogle Scholar
  9. Davies KJA, Packer L, Brooks GA (1981) Biochemical adaptation of mitochondria, muscle and whole animal respiration to endurance traning. Arch Biochem Biophys 209: 539–554PubMedCrossRefGoogle Scholar
  10. Depocas F, Minaire Y, Chatonnet J (1969) Rates of formation and oxidation of lactic acid in dogs at rest and during moderate exercise. Can J Physiol Pharmacol 47: 603–610PubMedCrossRefGoogle Scholar
  11. Donovan CM, Brooks GA (1983) Training affects lactate clearance, not lactate production. Am J Physiol 244 (Endocrinol Metab 7): E83–E92PubMedGoogle Scholar
  12. Drury DR, Wick AN (1956) Metabolism of lactic acid in the intact rabbit. Am J Physiol 184: 304–308PubMedGoogle Scholar
  13. Eldridge FL (1975) Relationship between turnover and blood concentration in exercising dogs. J Appl Physiol 39: 231–234PubMedGoogle Scholar
  14. Everse J, Kaplan NO (1973) Lactate dehydrogenases: structure and function. Adv Enzymol 37: 61–134PubMedGoogle Scholar
  15. Fitts RH, Booth FW, Winder WW, Holloszy JO (1975) Skeletal muscle respiratory capacity, endurance, and glycogen utilization. Am J Physiol 228: 1029–1033PubMedGoogle Scholar
  16. Freminet A, Le Clerc L (1980) Effect of fasting on glucose lactate and alanin turnover in rats and guinea pigs. Comp Biochem Physiol 65: 363–367Google Scholar
  17. Freminet A, Bursax E, Poyart CB (1972) Mesure de la vitesse de renouvellement du lactate chez lerat per perfusion de 14C-lactate. Pflügers Arch 334: 292–297CrossRefGoogle Scholar
  18. Gaesser GA, Brooks GA (1984) Metabolic bases of excess post-exercise oxygen consumption: a review. Med Sci Sports Exercise 16: 29–43Google Scholar
  19. Green HJ, Hughson RL, Orr GW, Ranney DA (1983) Anaerobic threshold, blood lactate, and muscle metabolites in progressive exercise. J Appl Physiol: Respir Environ Exercise Physiol 54: 1032–1038Google Scholar
  20. Hetenyi G, Perez G, Vranic M (1983) Turnover and precursor-product relationships of nonlipid metabolites. Physiol Rev 63: 606–667PubMedGoogle Scholar
  21. Issekutz B Jr (1970) Studies on hepatic glucose cycles in normal and methylprednisolone treated dogs. Metabolism 26: 157–170CrossRefGoogle Scholar
  22. Issekutz B Jr (1978) Role of beta-adrenergic receptors in mobilization of energy sources in exercising dogs. J Appl Physiol:Respir Environ Exercise Physiol 44: 869–876Google Scholar
  23. Issekutz B Jr, Paul P, Miller HI (1967) Metabolism in normal and pancreatomized dogs during steady-state exercise. Am J Physiol 213: 857–860PubMedGoogle Scholar
  24. Issekutz B Jr, Shaw WAS, Issekutz AC (1976) Lactate metabolism in resting and exercising dogs.217 J Appl Physiol 40: 312–319Google Scholar
  25. Jöbsis FF, Stainsby WN (1968) Oxidation of NADH during contractions of circulated mammalian skeletal muscle. Respir Physiol 4: 292–300PubMedCrossRefGoogle Scholar
  26. Karagiorogos A, Garcia JF, Brooks GA (1979) Growth hormone response to continuous and intermittent exercise. Med Sci Sport 11: 302–307Google Scholar
  27. Karlsson J, Hulten B, Sjodin B (1974) Substrate activation and product inhibition of LDH activity in human skeletal muscle. Acta Physiol Scand 92: 21–26PubMedCrossRefGoogle Scholar
  28. Katz JF, Okajima F, Chenoweth M, Dunn A (1981) The determination of lactate turnover in vivo with 3H and 14-labelled lactate. Biochem J 194: 513–524PubMedGoogle Scholar
  29. Kreisberg RA, Pennington LF, Boshell BR (1970) Lactate turnover and gluconeogenesis in normal and obese humans. Diabetes 19: 53–63PubMedGoogle Scholar
  30. Lehmann M, Wybitul K, Spori U, Keul J (1982) Catecholamines, cardiocirculatory and metabolic response during graduated and continuously increasing exercise. Int Arch Occup Environ Health 50: 261–271PubMedCrossRefGoogle Scholar
  31. Lehninger AL (1975) Biochemistry: The molecular basis of cell structure and function. Worth, New York, p 431Google Scholar
  32. Lusk G (1924) Analysis of the oxidation of mixtures of carbohydrate and fat. J Biol Chem 59: 41–42Google Scholar
  33. Mazzeo RS, Brooks GA, Budinger TF, Seholler DA (1982) Puise injection, 13C tracer studies of lactate metabolism in humans during rest ad two levels of exercise. Biomed Mass Spectrom 9: 310–314PubMedCrossRefGoogle Scholar
  34. Mole PA, Baldwin KM, Terjung RL, Holloszy JO (1973) Enzymatic pathways of pyruvate metabolism in skeletal muscle: adaptations to exercise. Am J Physiol 224: 50–54PubMedGoogle Scholar
  35. Newsholme EA, Leech AR (1983) Biochemistry for the Médical Sciences. John Wiley, ChichesterGoogle Scholar
  36. Nisselbaum JS, Packer DE, Bodansky O (1964) Comparison of the actions of human, brain, liver, and heart lactic dehydrogenase variants on nucleotide analogs on the substrate analogues in the absence and in the presence of oxalate and oxanmate. J Biol Chem 239: 2830–2834PubMedGoogle Scholar
  37. Pirnay F, Lamy M, Dujardin J, Deroanne R, Petit JM (1972) Analysis of femoral venous blood during maximum muscular exercise. J Appl Physiol 33: 289–292PubMedGoogle Scholar
  38. Reilly PKR, Chandrasena LG (1977) Sheep lactate entry-rate measurements: error due to sampling jugular blood. Am J Physiol (Endocrinol Metab Gasterointest Physiol 2 ): E138–E140Google Scholar
  39. Richter EA, Ploug T, Galbo H (1984) Increased muscle glucose uptake during contractions: no need for insulin. Med Sci Sports Exercise 16: 173Google Scholar
  40. Searle GL, Cavalieri RR (1972) Determination of lactate kinetics in the human analysis of data from single injection vs. continuous infusion methods. Proc Soc Exp Biol Med 139: 1002–1006PubMedGoogle Scholar
  41. Stanley WC, Gertz EW, Wisneski JA, Neese RA, Brooks GA (1984) Glucose and lactate turnover in man during rest and exercise studied with simultaneous infusion of 14C-glucose and 13C-lactate. Med Sci Sports Exercise 16: 136Google Scholar
  42. Winder WW, Hagberg JM, Hickson RC, Ehsani AA, McLane JA (1978) Time course of sympathoadrenev adaptation to endurance exercise training in man. J Appl Physiol 45: 370–374PubMedGoogle Scholar
  43. York J, Oscai LB, Penny DG (1974) Alterations in skeletal muscle lactic dehydrogenase isozymes following exercise traning. Biochem Biophys Res Commun 61: 1387–1393PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • G. A. Brooks
    • 1
  1. 1.Exercise Physiology Laboratory, Department of Physical EducationUniversity of CaliforniaBerkeleyUSA

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