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Cell Death Caused by the Anti-Cancer Drug Methotrexate: Does ADP-Ribosyl Transferase Have a Role to Play?

  • Kevin M. Prise
  • Jozsef C. Gaal
  • Colin K. Pearson
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Thymidylate residues required for cellular DNA synthesis are obtained exogenously via the salvage pathway and/or are synthesised de novo via dUMP in a reaction catalysed by thymidylate synthase (dUMP → dTMP). The thymidine monophosphate can then be phosphorylated by kinases to dTDP and dTTP before being incorporated into DNA.

Keywords

Transferase Activity Sodium Bisulphite Thymidine Monophosphate Sucrose Gradient Sedimentation Uracil Incorporation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Kevin M. Prise
  • Jozsef C. Gaal
  • Colin K. Pearson
    • 1
  1. 1.Department of Biochemistry, Marischal CollegeUniversity of AberdeenAberdeenScotland, Great Britain

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