Cell Death Caused by the Anti-Cancer Drug Methotrexate: Does ADP-Ribosyl Transferase Have a Role to Play?

  • Kevin M. Prise
  • Jozsef C. Gaal
  • Colin K. Pearson
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Thymidylate residues required for cellular DNA synthesis are obtained exogenously via the salvage pathway and/or are synthesised de novo via dUMP in a reaction catalysed by thymidylate synthase (dUMP → dTMP). The thymidine monophosphate can then be phosphorylated by kinases to dTDP and dTTP before being incorporated into DNA.


Transferase Activity Sodium Bisulphite Thymidine Monophosphate Sucrose Gradient Sedimentation Uracil Incorporation 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Kevin M. Prise
  • Jozsef C. Gaal
  • Colin K. Pearson
    • 1
  1. 1.Department of Biochemistry, Marischal CollegeUniversity of AberdeenAberdeenScotland, Great Britain

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