Abstract
Poly(ADP-ribose) metabolism is a chromatin associated event. The function of poly-(ADP-ribose) may be mediated by activation or inactivation of enzymatic activities by the covalent binding of the polymer and/or by noncovalent interactions of this highly negatively charged polymer with other chromatin components. We have studied the size distribution and branching frequency of polymers in order to evaluate the potential importance on noncovalent interactions with other components of chromatin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- HPLC:
-
High performance liquid chromatography
- (PR)2 AMP:
-
2′-[1″-ribosyl2″(1′″-ribosyl)]adenosine 5′,5″,5′″ Tris-(phosphate)
- PRAMP:
-
2′-(1″-ribosyl)adenosine 5′,5″ bis(phosphate)
- AMP:
-
adenosine 5′monophosphate
- EDTA:
-
Ethylenediaminotetraacetic acid
- temed:
-
N,N,N′,N′ tetramethylethylenediamine
References
Alvarez-Gonzalez R, Juarez-Salinas H, Jacobson EL, Jacobson MK (1983) Evaluation of immobilized boronates for studies of adenine and pyridine nucleotide metabolism. Anal Biochem 135:69–77
Sugimura T, Miwa M (1982) Structure and properties of poly(ADP-ribose). In: Hayaishi O, Ueda K (eds) ADP-ribosylation reactions. Academic Press, London New York, p 43
Juarez-Salinas H, Duran-Torres G, Jacobson MK (1984) Alteration of poly(ADP-ribose) by hyperthermia. Biochem Biophys Res Commun 122:1381–1388
Jacobson MK, Payne DM, Alvarez-Gonzalez R, Juarez-Salinas H, Sims JL, Jacobson EL (1984) Determination of in vivo levels of polymeric and monomeric ADP-ribose by fluorescence methods. Methods Enzymol 106:483–494
Butler PJG (1983) The folding of chromatin. CRC Crit Rev Biochem 15:57–91
Sau P, Bradbury EM, Baldwin JP (1979) Higher ordered structures of chromatin in solution. Eur J Biochem 97:593–599
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Alvarez-Gonzales, R., Jacobson, M.K. (1985). Size Distribution of Branched Polymers of ADP-Ribose Generated in Vitro and in Vivo. In: Althaus, F.R., Hilz, H., Shall, S. (eds) ADP-Ribosylation of Proteins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70589-2_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-70589-2_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-70591-5
Online ISBN: 978-3-642-70589-2
eBook Packages: Springer Book Archive