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Effect of DNA Intercalators on Poly(ADP-Ribose) Glycohydrolase Activity

  • Manoochehr Tavassoli
  • Sydney Shall
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Poly(ADP-ribose) glycohydrolase is the major enzyme responsible for the degradation of poly(ADP-ribose) [1, 2]. This enzyme hydrolyses the ribosyl-ribose bond between the two ADP-ribose units and produces ADP-ribose. We have purified this enzyme to near homogeneity and characterised its properties [2].

Keywords

Ethidium Bromide Phosphodiesterase Activity Cancer Research Campaign Snake Venom Phosphodiesterase Histone Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Ethacridine

2-thoxy-6,9 acridine diamine

m-AMSA

4′-(9-acridinylamino) methanesulphon-m-anisidide

Tilorone R10,556 DA

2,7-Bis(4-piperidinobutyryl)-9H-fluoren-9-one

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References

  1. 1.
    Miyakawa N, Ueda K, Hayaishi O (1972) Association of poly(ADP-ribose) glycohydrolase with liver chromatin. Biochem Biophys Acta Res Commmun 49:239–245CrossRefGoogle Scholar
  2. 2.
    Tavassoli M, Tavassoli MH, Shall S (1983) Isolation and purification of poly(ADP-ribose) glycohydrolase from pig thymus. Eur J Biochem 135:449–455PubMedCrossRefGoogle Scholar
  3. 3.
    Gale EF, Cundliffe E, Reynolds PE, Richmond MH, Waring MJ (1981) The classical intercalating drugs. In: The molecular basis of antibiotic action, 2nd edn. Wiley, London New York, pp 274–306Google Scholar
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    Durkacz BW, Omidiji O, Gray DA, Shall S (1980) (ADP-ribose)n participates in excision repair. Nature (London) 283:593–596CrossRefGoogle Scholar
  5. 5.
    Farzaneh F, Zalin R, Brill D, Shall S (1982) DNA strand breaks and ADP-ribosyl transferase activation during cell differentiation. Nature (London) 300:362–366CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Manoochehr Tavassoli
    • 1
  • Sydney Shall
    • 1
  1. 1.Cell and Molecular Biology LaboratoryUniversity of SussexBrighton, East SussexGreat Britain

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