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Kinetic Mechanism of Poly(ADP-Ribose) Polymerase

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ADP-Ribosylation of Proteins

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Poly(ADP-ribose) polymerase is a chromatin-associated enzyme which, in the presence of fragmented DNA, assembles branched homopolymers from the ADP-ribose moiety of NAD (reviewed in [1]). Fragmented DNA is an essential activator of the polymerase and is not modified by the reaction. This report presents data related to the kinetic mechanism of DNA activation of the poly(ADP-ribose) polymerase.

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Abbreviations

poly(dT):

poly(deoxythymidylic acid)

EDTA:

ethylenediamine tetraacetic acid

References

  1. Pekala PH, Moss J (1983) Poly(ADP-ribosylation) of protein. Cur Top Cell Regul 22:1–49

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  2. Benjamin RC, Gill DM (1980) Poly(ADP-ribose) synthesis in vitro programmed by damaged DNA: A comparison of DNA molecules containing different types of strand breaks. J Biol Chem 255:10502–10508

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  3. Niedergang C, Okazaki H, Mandel P (1979) Properties of purified calf thymus poly(ADP-ribose) polymerase. Eur J Biochem 102:43–57

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  4. Cleland WW (1963) Statistical analysis of enzyme kinetic data. Methods Enzymol 63:103–138

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Texas College of Osteopathic Medicine, North Texas State University, Denton, TX, 76203, USA

    Robert C. Benjamin, Paul F. Cook & Myron K. Jacobson

Authors
  1. Robert C. Benjamin
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  2. Paul F. Cook
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  3. Myron K. Jacobson
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Editor information

Editors and Affiliations

  1. Institut für Pharmakologie und Biochemie, Veterinärmedizinische Fakultät der Universität, CH-8057, Zürich, Switzerland

    Felix R. Althaus

  2. Physiologisch-chemisches Institut, Universität Hamburg, 2000, Hamburg 20-40, Germany

    Helmuth Hilz

  3. The University of Sussex, Biology Building, Brighton, Sussex, BN1 9QG, England

    Sydney Shall

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© 1985 Springer-Verlag Berlin Heidelberg

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Benjamin, R.C., Cook, P.F., Jacobson, M.K. (1985). Kinetic Mechanism of Poly(ADP-Ribose) Polymerase. In: Althaus, F.R., Hilz, H., Shall, S. (eds) ADP-Ribosylation of Proteins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70589-2_12

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  • DOI: https://doi.org/10.1007/978-3-642-70589-2_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70591-5

  • Online ISBN: 978-3-642-70589-2

  • eBook Packages: Springer Book Archive

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