Molecular Cloning of Genes Encoding Gram-Positive Virulence Factors

  • M. S. Gilmore
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 118)


Among both scientific and commercial reasons for cloning genes related to bacterial virulence, the most compelling is the contribution that analysis of cloned virulence determinants can make to advancing an understanding of infectious disease. Establishment of an infectious disease is the result of an imbalance in the steady-state “host-parasite” relationship. As the host defense mechanisms are many and interrelated, the ability of a bacterium to invade and cause disease is the result of the complex interaction of many bacterial traits. To understand bacterial pathogenicity, then, it is essential to isolate and characterize the components of this multifactorial process, identify regulatory features, and assess their respective contribution to the infectious disease. Recombinant DNA technology provides the necessary tool for isolating the genes encoding virulence factors.


Molecular Cloning Hemolytic Activity Shuttle Vector Diphtheria Toxin Bacteriocin Activity 
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© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • M. S. Gilmore
    • 1
    • 2
  1. 1.Department of Microbiology and ImmunologyUniversity of Oklahoma, Health Sciences CenterOklahomaUSA
  2. 2.The Dental Research Institute, School of DentistryThe University of MichiganAnn ArborUSA

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