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Ion Measurements in Physiology and Medicine pp 158–165Cite as

Thiocyanate Interference at Chloride-Selective Microelectrodes in Crayfish Stretch Receptor Neurons: Evidence for a Non-passive Thiocyanate Distribution

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Abstract

Since the introduction of ion-selective microelectrodes (SME) by Walker [19], a wealth of evidence has been accumulated indicating that the transmembrane Cl− gradient is not in equilibrium with the membrane potential E m . Depending upon the preparation, both higher (e.g., sheep heart Purkinje fibers [18]; guinea pig vas deferens [1]) and lower intracellular Cl− levels (e.g., crayfish stretch receptor [7,9a]) than those expected from a passive distribution have been measured. A low intracellular Cl− activity a icl , maintained by Cl− extrusion, appears to be of particular significance in those neurons where the driving force for inhibitory postsynaptic potentials (i. p. s. p.) is provided by the Cl− gradient.

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Authors
  1. R. A. Deisz
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  2. H. D. Lux
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Editor information

Editors and Affiliations

  1. Institut för Physiologie und Kardiologie, Waldstr. 6, D-8520, Erlangen, Germany

    Manfred Kessler , David Keith Harrison  & Jens Höper ,  & 

  2. Dept. of Chemistry, University of North Carolina, Chapel Hill, NC, 27514, USA

    R. P. Buck

  3. Dept. of Physiology, School of Medicine, The Center for Health Science, University of California, Los Angeles, California, 90024, USA

    G. Eisenman

  4. Biosensors Inc., PO Box 640, Chester, New Jersey, 07930, USA

    J. Hill

  5. Faculty of Medicine, American Univ. of Beirut, Beirut, Lebanon

    R. N. Khuri

  6. Direktor des Max-Planck-Instituts für Systemphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, Germany

    D. W. Lübbers

  7. Medical Center, Dept. of Physiology and Biophysics, New York University, 550 First Avenue, New York, NY, 10016, USA

    C. Nicholson

  8. Laboratorium für Organische Chemie, Universitätsstr. 16, 6000, Frankfurt M. 70, Germany

    W. Simon

  9. The Middlesex Hospital, Mortimer St., London W N, 8AA, England

    T. Treasure

  10. Institute of Medical Physiology, Dept. A, University of Copenhagen, DK-2200, Copenhagen N, Denmark

    T. Zeuthen

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© 1985 Springer-Verlag Berlin Heidelberg

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Deisz, R.A., Lux, H.D. (1985). Thiocyanate Interference at Chloride-Selective Microelectrodes in Crayfish Stretch Receptor Neurons: Evidence for a Non-passive Thiocyanate Distribution. In: Kessler, M., et al. Ion Measurements in Physiology and Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70518-2_25

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  • DOI: https://doi.org/10.1007/978-3-642-70518-2_25

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-15468-6

  • Online ISBN: 978-3-642-70518-2

  • eBook Packages: Springer Book Archive

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