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Biological Activities of a Human Pluripotent Hemopoietic Colony-Stimulating Factor

  • E. Platzer
  • K. Welte
  • L. Lu
  • J. L. Gabrilove
  • Y.-P. Yung
  • C. F. Nathan
  • R. Mertelsmann
  • M. A. S. Moore
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 29)

Abstract

As described elsewhere in this volume by Welte et al., a human pluripotent hemopoietic colony-stimulating factor (CSF) was purified to apparent homogeneity from media conditioned by the human bladder Carcinoma cell line, 5637. The purified material supports colony formation in vitro from human multipotential (CFUGEMM), early erythroid (BFU-E), granulocyte and monocyte (CFU-G, M) progenitor cells4. A murine CSF, Interleukin 3 or multi-CSF, with similar activities on normal mouse bone marrow [1, 2], has recently been purified [3] and genetically cloned [4]. Interleukin 3 was originally detected by its ability to induce 20-alpha-C) H-steroid dehydrogenase (20aSDH) in cultured spieen cells of nu/nu mice [5]. Later in it was discovered to have biological activities on wide range of hemopoietic cells: progenitor cells of erythrocytes, megakaryocytes, granulocytes, monocytes and eosinophils, mast cells and their precursors, and possibly lymphocytes [2, 6–8]. Since come activities of Interleukin 3 and pluripotent CSF, or Pluripoietin, on hemopoietic progenitor cells appeared similiar, we screened for additional biological effects of Pluripoietin.

Keywords

Hemopoietic Cell Normal Human Bone Marrow Murine Mast Cell Hemopoietic Progenitor Cell Human Bladder Carcinoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • E. Platzer
    • 1
  • K. Welte
    • 2
  • L. Lu
    • 2
  • J. L. Gabrilove
    • 2
  • Y.-P. Yung
    • 2
  • C. F. Nathan
    • 3
  • R. Mertelsmann
    • 2
  • M. A. S. Moore
    • 2
  1. 1.Institut f. klin. ImmunologieErlangenGermany
  2. 2.Laboratory of Developmental Hematopoiesis, and Laboratory of Molecular HematologyMemorial Sloan-Kettering Cancer CenterUSA
  3. 3.Laboratory of Cellular Physiology and ImmunologyThe Rockefeller UniversityNew YorkUSA

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