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Functions of the Outer Membrane Proteins of Neisseria gonorrhoeae

  • M. S. Blake
Part of the Bayer-Symposium book series (BAYER-SYMP, volume 8)

Summary

The functional aspects of purified major outer membrane proteins of Neisseria gonorrhoeae are described. These proteins are isolated in a novel way using calcium, zwitterionic detergent, and ethanol. By chemical analysis and amino terminal sequence, most of the proteins fall into three major families. Evidence will be given that would suggest that the protein II associated with opacity functions is in a receptor-ligand relationship with a portion of the lipopolysaccharide carbohydrate moiety. Also, evidence will be presented for the possible roles which the transfer of protein I from the gonococcal outer membrane to other membranes may have in the infectious process.

Keywords

Electron Spin Resonance Outer Membrane Electron Spin Resonance Spectrum Outer Membrane Protein Neisseria Gonorrhoeae 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Nikaido H (1979) Nonspecific transport through the outer membrane. In: Inouye M (ed) Bacterial outer membranes. Wiley, New York, pp 361–407Google Scholar
  2. 2.
    Blake MS, Gotschlich EC, Swanson JL (1981) The effect of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun 33: 212–222PubMedGoogle Scholar
  3. 3.
    Newhall WJ, Sawyer WD, Haak RA (1980) Cross-linking analysis of the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun 28: 785–791PubMedGoogle Scholar
  4. 4.
    McDade RL Jr, Johnston KH (1980) Characterization of serologically dominant outer membrane proteins of Neisseria gonorrhoeae. J Bacteriol 141: 1183–1191PubMedGoogle Scholar
  5. 5.
    Swanson J (1981) Surface-exposed protein antigens of the gonococcal outer membrane. Infect Immun 34: 804–816PubMedGoogle Scholar
  6. 6.
    Blake MS, Gotschlich EC (1984) Purification and partial characterization of the opacity-associated proteins of Neisseria gonorrhoeae. J Exp Med 159: 452–462PubMedCrossRefGoogle Scholar
  7. 7.
    James JF, Swanson J (1978) Studies on gonococcus infection: XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun 19: 332–340PubMedGoogle Scholar
  8. 8.
    Swanson J (1978) Studies on gonococcus infection: XIV. Cell wall protein differences among color/opacity colony variants of N. gonorrhoeae. Infect Immun 21: 292–302PubMedGoogle Scholar
  9. 9.
    Swanson J, Sparks E, Young D, King G (1975) Studies on gonococcus infection: X. Pili and leukocyte association factor as mediators of interactions between gonococci and eukaryotic cells in vitro. Infect Immun 11: 1352–1361PubMedGoogle Scholar
  10. 10.
    King GJ, Swanson J (1978) Studies on gonococcus infection: XV. Identification of surface proteins of Neisseria gonorrhoeae correlated with leukocyte association. Infect Immun 21: 575–584PubMedGoogle Scholar
  11. 11.
    Wong TP, Johnston KH (1981) One-dimensional peptide mapping of the major outer membrane protein of Neisseria gonorrhoeae. Infect Immun 34: 739–745PubMedGoogle Scholar
  12. 12.
    Montal M, Darszon A, Trisel HW (1977) Transmembrane channel formation in rhodopsin-containing bilayer membranes. Nature 267: 221–225PubMedCrossRefGoogle Scholar
  13. 13.
    Greco F (1981) Thesis. Rockefeller University, New YorkGoogle Scholar
  14. 14.
    Young JD-E, Blake M, Mauro A, Cohn ZA (1983) Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Proc Natl Acad Sci USA 80: 3831–3835PubMedCrossRefGoogle Scholar
  15. 15.
    Lynch EC, Blake MS, Gotschlich EC, Mauro A (1983) Studies of porins: Spontaneously transferred from whole cells and reconstituted from purified proteins of Neisseria gonorrhoeae and Neisseria meningitidis. Biophys J 45: 104–107CrossRefGoogle Scholar
  16. 16.
    Bouma SR, Drislane FW, Huestis WH (1977) Selective extraction of membrane-bound proteins by phospholipid vesicles. J Biol Chem 252: 6759–6763PubMedGoogle Scholar
  17. 17.
    Cook SL, Bouma SR, Huestis WH (1980) Cell to vesicle transfer of intrinsic membrane proteins: effect of membrane fluidity. Biochemistry 19: 4601–4607PubMedCrossRefGoogle Scholar
  18. 18.
    Enoch HG, Fleming PJ, Strittmatter P (1979) The binding of cytochrome b5 to phospholipid vesicles and biological membranes. J Biol Chem 254: 6483–6488PubMedGoogle Scholar
  19. 19.
    Huestis WH (1977) A sodium-specific membrane permeability defect induced by phospholipid vesicle treatment of erythrocytes. J Biol Chem 252: 6764–6768PubMedGoogle Scholar
  20. 20.
    Roseman MA, Holloway PW, Calabro MA, Thompson TE (1977) Exchange of cytochrome b5 between phospholipid vesicles. J Biol Chem 252: 4842–4849PubMedGoogle Scholar
  21. 21.
    Newton AC, Cook SL, Huestis WH (1983) Transfer of band 3, the erythrocyte anion transporter, between phospholipid vesicles. Biochemistry 22: 6110–6117PubMedCrossRefGoogle Scholar
  22. 22.
    Gallin EK, Gallin JI (1977) Interaction of chemotactic factors with human macrophages induction of transmembrane potential changes. J Cell Biol 75: 277–289PubMedCrossRefGoogle Scholar
  23. 23.
    Korchak HM, Weissmann G (1978) Changes in membrane potential of human granulocytes antecede the metabolic responses to surface stimulation. Proc Natl Acad Sci USA 75: 3818–3822PubMedCrossRefGoogle Scholar
  24. 24.
    Seligman BE, Gallin JI (1980) Use of lipophilic probes of membrane potential to assess human neutrophil activation. J Clin Invest 66: 493–503CrossRefGoogle Scholar
  25. 25.
    Whitin JC, Chapman CE, Simons ER, Chovaniec ME, Cohen HJ (1980) Correlation between membrane potential changes and superoxide production in human granulocytes stimulated by phorbal myristate acetate. J Biol Chem 255: 1874–1878PubMedGoogle Scholar
  26. 26.
    Young JD-E, Unkeless JC, Kaback HR, Cohn ZA (1983) Macrophage membrane potential changes associated with Fc receptor ligand binding. Proc Natl Acad Sci USA 80: 1357–1361PubMedCrossRefGoogle Scholar
  27. 27.
    Schleicher M, Noegel A, Watterson DM, Gotschlich EC (1983) Calmodulin, a eukaryotic regulator protein, binds in vitro to outer membrane proteins from neisseria species. Fed Proc 42: 861Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • M. S. Blake

There are no affiliations available

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