Coupling of Glycolytic Oscillations and Convective Patterns
When a spatially extended light beam passes through a thin layer of yeast extract placed in a petri dish, a wavelength specific for the absorption of reduced nicotinamide adenine dinucleotide (NADH) is selected, and when glycolysis in the cytoplasmic medium proceeds in an oscillatory manner, spatial patterns are observed on a length scale comparable to the layer depth (1 to 2 mm). They form and disappear periodically in intervals of several minutes ill. Further insight into the coupling of the oscillatory degradation of glycolytic substrates such as glycogen and transport processes in a liquid layer is gained with a two-dimensional UV sensitive spectrophotometer, which uses computer-based video techniques for fast recording of spatially resolved light intensities . The transmission experiments are combined with a dark-field type optical method for detecting spatial distributions of refractive index gradients.
KeywordsMethyl Orange Transmitted Light Convection Cell Marangoni Convection Transmission Pattern
Unable to display preview. Download preview PDF.
- 1.A. Boiteux and B. Hess, Ber. Bunsenges. Phys. Chem. 84, 392 (1980)Google Scholar
- 2.S.C. Müller, Th. Plesser and B. Hess, Anal. Biochem. (in press)Google Scholar
- 4.S.C. Müller, Th. Plesser and B. Hess, Naturwissenschaften (in press)Google Scholar
- 6.Convective Transport and Instability Phenomena (J. Zierep and H. Örtel, eds.) G. Braun, Karlsruhe (1982)Google Scholar
- 11.S.C. Müller and Th. Plesser, in: Modelling of Patterns in Space and Time (W. Jäger and J. Murray, eds.) Springer, Berlin (1984).Google Scholar