Coupling of Glycolytic Oscillations and Convective Patterns
When a spatially extended light beam passes through a thin layer of yeast extract placed in a petri dish, a wavelength specific for the absorption of reduced nicotinamide adenine dinucleotide (NADH) is selected, and when glycolysis in the cytoplasmic medium proceeds in an oscillatory manner, spatial patterns are observed on a length scale comparable to the layer depth (1 to 2 mm). They form and disappear periodically in intervals of several minutes ill. Further insight into the coupling of the oscillatory degradation of glycolytic substrates such as glycogen and transport processes in a liquid layer is gained with a two-dimensional UV sensitive spectrophotometer, which uses computer-based video techniques for fast recording of spatially resolved light intensities . The transmission experiments are combined with a dark-field type optical method for detecting spatial distributions of refractive index gradients.
KeywordsConvection Peri NADH Adenine Dinucleotide
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