Isolation of Plasma Membrane from Ryegrass (Lolium multiflorum) Endosperm Protoplasts
Biochemical studies on the structure an function of plant plasma membranes have been hampered because satisfactory methods of their isolation have not been available. A major reason is that the high shear forces required to disrupt plant cell walls cause considerable membrane fragmentation and damage to cell organelles. Isolation of plasma membranes from the resulting homogenate was rendered particularly difficult by the lack of unequivocal markers for their identification (Leonard and Hodges 1980) and because they comprise only a small proportion of the total cellular membranes (Morré and Buckhout 1979).
KeywordsSucrose Fractionation Sarcoma Sponge Histidine
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